Coexpression of hypoxia-inducible factor 1-alpha and vascular endothelial growth factor in Wilms' tumor

Karth, John; Ferrer, Fernando; Perlman, Elizabeth J.; Hanrahan, Collen; Simons, Jonathan W.; Gearhart, John P.; Rodríguez, Ronald

doi:10.1053/jpsu.2000.19241

Cited by 31 publications

(30 citation statements)

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“…In fact, based on the analysis of HIF-1 pathway, we have learned that, under hypoxic conditions, HIF-1α protein is synthesized and accumulated in cytoplasm, then translocates into nucleus, dimerizes with HIF-1beta, recruits the coactivators p300/CBP, and regulates expression of its transcriptional targets via binding to hypoxia-response elements. Staining of HIF-1α within the cytoplasm might represent diffusion of the protein from within the nucleus, or sequestration of an inactive pool [30] .…”

Section: Discussionmentioning

confidence: 99%

Expression of hypoxia-inducible factor-1α in liver tumors after transcatheter arterial embolization in an animal model

Liang

Zheng

Gao

et al. 2009

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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To examine the effect of transcatheter arterial embolization (TAE) of liver tumors on hypoxia-inducible factor-1alpha (HIF-1alpha) expression in the residual viable tumor, a total of 30 New Zealand White rabbits implanted with VX2 liver tumor were divided into 2 groups. TAE-treated group animals (n=15) were subjected to TAE with 150-250 microm polyvinyl alcohol particles. Control group animals (n=15) underwent sham embolization with distilled water. Six hours, 3 days or 7 days after TAE, the animals were sacrificed, and samples of tumor and adjacent normal liver tissue were harvested. Expression of HIF-1alpha protein was examined immunohistochemically. Real-time PCR was performed to examine the HIF-1alpha mRNA levels. Our results showed that HIF-1alpha protein was expressed in the VX2 tumors but not in the adjacent normal liver tissue. The HIF-1alpha-positive tumor cells were located predominantly at the periphery of necrotic tumor regions. The mean levels of HIF-1alpha protein were significantly higher in TAE-treated tumors than those in control tumors (P=0.002). Among the three sacrificing time points, the difference in increase in HIF-1alpha protein was significant between the two groups at the sacrificing time point of 6 h and 3 days after TAE (P=0.020, P=0.031, respectively), whereas no significant increase was noted 7 days after TAE (P=0.502). In contrast, although HIF-1alpha mRNA was expressed in TAE-treated and control VX2 tumors, there existed no significant difference in the HIF-1alpha mRNA level between the two groups (P=0.372). It is concluded that TAE of liver tumors increases the expression of HIF-1alpha at protein level in the residual viable tumor, which could be attributed to hypoxia generated by the procedure.

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Section: Discussionmentioning

confidence: 99%

Expression of hypoxia-inducible factor-1α in liver tumors after transcatheter arterial embolization in an animal model

Liang

Zheng

Gao

et al. 2009

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…WT1 is an essential regulator of nephrogenesis (40)(41)(42) and has been suggested to play an important regulatory role in angiogenesis (34,39,43). WT1 and VEGF are co-expressed in normal podocytes, but also in some Wilms' tumors (42)(43)(44). In addition to the kidney, WT1 is expressed in many other organs (20), including hematopoietic tissues such as the spleen, fetal liver, and bone marrow (17,45,46) where tightly regulated VEGF expression is required.…”

Section: Discussionmentioning

confidence: 99%

Regulation of vascular endothelial growth factor, VEGF, gene promoter by the tumor suppressor, WT1

Hanson

Gorman²,

Reese³

et al. 2007

Front Biosci

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Understanding angiogenesis and growth control is central for elucidating prostate tumorigenesis. However, the mechanisms of activation of the angiogenic gene, vascular endothelial growth factor (VEGF) are complex and its regulation in prostate cancer is not well understood. In previous studies, VEGF expression levels were correlated with altered levels of the zinc finger transcription factor, WTl. Since the VEGF promoter has several potential WTl binding sites and WT1 regulates many growth control genes, here we assessed whether WTl might also regulate VEGF transcription. Using transfection and DNA binding assays, functional WTl binding sites were localized within the proximal VEGF promoter. Transfection of the DDS-WTl (R394W) zinc finger mutant had no significant effect on VEGF-luciferase reporter activity, suggesting that an intact zinc finger DNA binding domain was required. Interestingly, WTl-mediated regulation of VEGF reporter constructs varied in different cell types. In androgen-responsive, LNCaP prostate cancer cells, hormone treatment enhanced WTl-mediated activation of the VEGF promoter constructs. Overall, these results suggest that WTl transcriptionally regulates VEGF through interaction of its zinc finger DNA binding domain with the proximal GC-rich VEGF promoter. These findings may shed light on the role of WTl in angiogenesis and prostate cancer progression.

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“…Research has shown that VEGF is expressed in many types of solid tumors (Ramakrishnan et al, 2000;Karth et al, 2000;Wong et al, 2003;Koukourakis et al, 2004), generally mediated by transcriptional activator known as hypoxia inducible factor 1 (HIF-1) in carcinoma (Forsythe et al, The third passage HUVEC were incubated with Gl-PP at a concentration of 10, 100 Ag ml À 1 for 48 h, 5% DMEM medium were used as control. Total protein was extracted.…”

Section: Discussionmentioning

confidence: 99%

Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell

2006

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Coexpression of hypoxia-inducible factor 1-alpha and vascular endothelial growth factor in Wilms' tumor

Cited by 31 publications

References 11 publications

Expression of hypoxia-inducible factor-1α in liver tumors after transcatheter arterial embolization in an animal model

Expression of hypoxia-inducible factor-1α in liver tumors after transcatheter arterial embolization in an animal model

Regulation of vascular endothelial growth factor, VEGF, gene promoter by the tumor suppressor, WT1

Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell

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