Coexpression of Two Odorant-Binding Protein Homologs in<i>Drosophila</i>: Implications for Olfactory Coding

Hekmat-Scafe, Daria S.; Steinbrecht, Rudolf Alexander; Carlson, John R.

doi:10.1523/jneurosci.17-05-01616.1997

Cited by 92 publications

(80 citation statements)

References 35 publications

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“…1), and the cluster of six Obp genes at 83C-D contains Or83c. Interestingly, similar expression patterns have been observed for Obp83a, Obp83b, and Or83c: All are found in sensory hairs on the ventro-lateral aspect of the antenna (McKenna et al 1994;Pikielny et al 1994;Hekmat-Scafe et al 1997;Vosshall et al 2000). On the other hand, the expression pattern of Or56a differs from those of the Obp56 genes (Vosshall et al 2000;Galindo and Smith 2001).…”

Section: The Drosophila Obp Gene Family Is Composed Of 51 Members Momentioning

confidence: 59%

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Genome-Wide Analysis of the Odorant-Binding Protein Gene Family in Drosophila melanogaster

Hekmat-Scafe¹,

Scafe²,

McKinney³

et al. 2002

Genome Res.

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413

404

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Olfaction is of considerable importance to many insects in behaviors critical for survival and reproduction, including location of food sources, selection of mates, recognition of colony con-specifics, and determination of oviposition sites. An ubiquitous, but poorly understood, component of the insect's olfactory system is a group of odorant-binding proteins (OBPs) that are present at high concentrations in the aqueous lymph surrounding the dendrites of olfactory receptor neurons. OBPs are believed to shuttle odorants from the environment to the underlying odorant receptors, for which they could potentially serve as odorant presenters. Here we show that the Drosophila genome carries 51 potential OBP genes, a number comparable to that of its odorant-receptor genes. We find that the majority (73%) of these OBP-like genes occur in clusters of as many as nine genes, in contrast to what has been observed for the Drosophila odorant-receptor genes. Two of the presumptive OBP gene clusters each carries an odorant-receptor gene. We also report an intriguing subfamily of 12 putative OBPs that share a unique C-terminal structure with three conserved cysteines and a conserved proline. Members of this subfamily have not previously been described for any insect. We have performed phylogenetic analyses of the OBP-related proteins in Drosophila as well as other insects, and we discuss the duplication and divergence of the genes for this large family.

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Section: The Drosophila Obp Gene Family Is Composed Of 51 Members Momentioning

confidence: 59%

“…Additional data on the gene products will be required to determine which of these 51 Obp genes encode bona fide OBPs (Steinbrecht et al 1992(Steinbrecht et al , 1995Du and Prestwich 1995;Ozaki et al 1995;Hekmat-Scafe et al 1997;Kim et al 1998;Park et al 2000;Plettner et al 2000).…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Analysis of the Odorant-Binding Protein Gene Family in Drosophila melanogaster

Hekmat-Scafe¹,

Scafe²,

McKinney³

et al. 2002

Genome Res.

Self Cite

413

404

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“…Within the third antennal segment however, AntDH expression appears uniformly distributed (Fig. 2 and data not shown), in contrast with several odorant-binding proteins, each of which is restricted to a single morphological type of sensillum with a nonuniform distribution on the antennal surface (21,26,27). 2 …”

Section: Several Biodegradation Enzymes Are Expressed Preferentially mentioning

confidence: 92%

Preferential Expression of Biotransformation Enzymes in the Olfactory Organs of Drosophila melanogaster, the Antennae

Wang¹,

Hasan

Pikielny³

1999

Journal of Biological Chemistry

113

102

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Biotransformation enzymes have been found in the olfactory epithelium of vertebrates. We now show that in Drosophila melanogaster, a UDP-glycosyltransferase (UGT), as well as a short chain dehydrogenase/reductase and a cytochrome P450 are expressed specifically or preferentially in the olfactory organs, the antennae. The evolutionarily conserved expression of biotransformation enzymes in olfactory organs suggests that they play an important role in olfaction. In addition, we describe five Drosophila UGTs belonging to two families. All five UGTs contain a putative transmembrane domain at their C terminus as is the case for vertebrate UGTs where it is required for enzymatic activity. The primary sequence of the C terminus, including part of the transmembrane domain, differs between the two families but is highly conserved not only within each Drosophila family, but also between the members of one of the Drosophila families and vertebrate UGTs. The partial overlap of the conserved primary sequence with the transmembrane domain suggests that this part of the protein is involved in specific interactions occurring at the membrane surface. The presence of different C termini in the two Drosophila families suggests that they interact with different targets, one of which is conserved between Drosophila and vertebrates.

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“…In addition to families of chemosensory receptors, a large family of odorant binding proteins (OBPs) is also differentially expressed (Hekmat-Scafe et al, 1997Park et al, 2000;Galindo and Smith, 2001;Shanbhag et al, 2001;Vogt et al, 2002). OBPs are secreted into the sensillar perilymph and, although their precise function has not been conclusively established, a prevailing hypothesis is that they facilitate transport of hydrophobic odorants through the perilymph to receptors at the chemosensory membrane (Kaissling, 1996;Du and Prestwich, 1995;Robertson et al, 1999;Vogt et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Pinocchio, a novel protein expressed in the antenna, contributes to olfactory behavior inDrosophila melanogaster

2005

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Most organisms depend on chemoreception for survival and reproduction. In Drosophila melanogaster multigene families of chemosensory receptors and putative odorant binding proteins have been identified. Here, we introduce an additional distinct protein, encoded by the CG4710 gene, that contributes to olfactory behavior. Previously, we identified through P[lArB]-element mutagenesis a smell impaired (smi) mutant, smi21F, with odorant-specific defects in avoidance responses. Here, we show that the smi21F mutant also exhibits reduced attractant responses to some, but not all, of a select group of odorants. Furthermore, electroantennogram amplitudes are increased in smi21F flies. Characterization of flanking sequences of the P[lArB] insertion site, complementation mapping, phenotypic reversion through P-element excision, and expression analysis implicate a predicted gene, CG4710, as the candidate smi gene. CG4710 produces two transcripts that encode proteins that contain conserved cysteines and which are reduced in the smi21F mutant. Furthermore, in situ hybridization reveals CG4710 expression in the third antennal segment. We have named this gene of previously unknown function and its product ''Pinocchio (Pino)''.

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Coexpression of Two Odorant-Binding Protein Homologs inDrosophila: Implications for Olfactory Coding

Cited by 92 publications

References 35 publications

Genome-Wide Analysis of the Odorant-Binding Protein Gene Family in Drosophila melanogaster

Genome-Wide Analysis of the Odorant-Binding Protein Gene Family in Drosophila melanogaster

Preferential Expression of Biotransformation Enzymes in the Olfactory Organs of Drosophila melanogaster, the Antennae

Pinocchio, a novel protein expressed in the antenna, contributes to olfactory behavior inDrosophila melanogaster

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