Cofermentation of Glucose, Xylose, and Arabinose by Genomic DNA-lntegrated Xylose/Arabinose Fermenting Strain of Zymomonas mobilis AX101

Mohagheghi, Ali; Evans, Kent; Chou, Yat‐Chen; Zhang, Min

doi:10.1007/978-1-4612-0119-9_72

Cited by 57 publications

(54 citation statements)

References 12 publications

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“…Therefore, catabolism of monosaccharides has been an important target in the development of an optimized microbial platform. Pathways for xylose and arabinose use have recently been engineered into Saccharomyces cerevisiae [8][9][10] and Zymomonas mobilis [11]. Although developments in sugar catabolism were made in the context of homo-ethanol production, the advances are applicable to the production of a variety of fuel molecules through multiple biosynthetic pathways (discussed below).…”

Section: Sugar Catabolism and The Fermentation Pathwaymentioning

confidence: 99%

Biofuel alternatives to ethanol: pumping the microbial well

Fortman

Chhabra

Mukhopadhyay

et al. 2008

Trends in Biotechnology

338

209

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Section: Sugar Catabolism and The Fermentation Pathwaymentioning

confidence: 99%

Biofuel alternatives to ethanol: pumping the microbial well

Fortman

Chhabra

Mukhopadhyay

et al. 2008

Trends in Biotechnology

338

209

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“…Despite this, wild Z. mobilis strains cannot use xylose, the major component of hemicellulose [4]. Hence, many efforts have been devoted to develop efficient xylose-fermenting Z. mobilis, and at present, several recombinant strains capable of fermenting xylose to ethanol have been engineered [5,6]. However, the ability of engineered strains to metabolize xylose is lower than their ability to metabolize glucose.…”

mentioning

confidence: 99%

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Dong

Zhao

et al. 2012

Chin. Sci. Bull.

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A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman (PB) design and response surface methodology (RSM). The effects of 19 medium components were investigated by PB design. Eight of these components were determined to have significant effects on ethanol production. The statistical model was constructed via central composite design (CCD) using five selected variables, including xylose, trisodium citrate, choline chloride, pyridoxine, and thiamine. The validity of the developed model was verified. The ethanol concentration in the optimized medium was 6.7% higher than in the RM medium. The optimized medium was then simplified to give the final synthetic medium (S2), in which the ethanol concentration was 20.7% higher than in the RM medium. Xylose and trisodium citrate were the key medium components influencing ethanol production, while calcium pantothenate was the only growth factor required by recombinant Z. mobilis. Ethanol production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.recombinant Zymomonas mobilis, synthetic medium, ethanol production, response surface methodology, trisodium citrate, xylose Citation:Dong H N, Zhao X M, Ma Y Y, et al. Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology.

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“…A xylose-fermenting Z. mobilis was engineered by introducing a xylose-metabolizing pathway from E. coli (Zhang et al, 1995); however, the ethanol tolerance appears to be significantly lower when fermenting a xylose/glucose mixture compared to glucose alone (Joachimsthal and Rogers, 2000). More recently, a xylose-and arabinose-fermenting strain of Z. mobilis was produced (Mohagheghi et al, 2002). This strain, AX101, used glucose, then xylose and finally arabinose in mixed-sugar fermentations, and was hampered by acetic acid toxicity.…”

Section: Ethanol (Ch 3 Ch 2 Oh; C 2 H 6 O)mentioning

confidence: 99%

“…This strain, AX101, used glucose, then xylose and finally arabinose in mixed-sugar fermentations, and was hampered by acetic acid toxicity. Further exploration of these engineered Z. mobilis biocatalysts is still of interest due to their ability to ferment at low pH and form minimal by-products (Mohagheghi et al, 2002).…”

Section: Ethanol (Ch 3 Ch 2 Oh; C 2 H 6 O)mentioning

confidence: 99%

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

2008

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SummaryConcerns for our environment and unease with our dependence on foreign oil have renewed interest in converting plant biomass into fuels and 'green' chemicals. The volume of plant matter available makes lignocellulose conversion desirable, although no single isolated organism has been shown to depolymerize lignocellulose and efficiently metabolize the resulting sugars into a specific product. This work reviews selected chemicals and fuels that can be produced from microbial fermentation of plant-derived cell-wall sugars and directed engineering for improvement of microbial biocatalysts. Lactic acid and ethanol production are highlighted, with a focus on engineered Escherichia coli.

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Cofermentation of Glucose, Xylose, and Arabinose by Genomic DNA-lntegrated Xylose/Arabinose Fermenting Strain of Zymomonas mobilis AX101

Cited by 57 publications

References 12 publications

Biofuel alternatives to ethanol: pumping the microbial well

Biofuel alternatives to ethanol: pumping the microbial well

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

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