Coffea arabica Extract Attenuates Atopic Dermatitis-like Skin Lesions by Regulating NLRP3 Inflammasome Expression and Skin Barrier Functions

Abstract

Atopic dermatitis (AD) is a common skin disease worldwide. The major causes of AD are skin barrier defects, immune dysfunction, and oxidative stress. In this study, we investigated the anti-oxidation and anti-inflammation effects of Coffea arabica extract (CAE) and its regulation of the skin barrier and immune functions in AD. In vitro experiments revealed that CAE decreased the reactive oxygen species levels and inhibited the translocation of nuclear factor-κB (NF-κB), further reducing the secretion of interleukin (IL)-1β and IL-6 induced by interferon-γ (IFN-γ)/tumor necrosis factor-α (TNF-α). Moreover, CAE decreased IFN-γ/TNF-α-induced NLR family pyrin domain-containing 3 (NLRP3), caspase-1, high-mobility group box 1 (HMGB1), and receptor for advanced glycation end products (RAGE) expression levels. It also restored the protein levels of skin barrier function-related markers including filaggrin and claudin-1. In vivo experiments revealed that CAE not only reduced the redness of the backs of mice caused by 2,4-dinitrochlorobenzene (DNCB) but also reduced the levels of pro-inflammatory factors in their skin. CAE also reduced transepidermal water loss (TEWL) and immune cell infiltration in DNCB-treated mice. Overall, CAE exerted anti-oxidation and anti-inflammation effects and ameliorated skin barrier dysfunction, suggesting its potential as an active ingredient for AD treatment.

Effect of Chloroquine on Type 2 Inflammatory Response in MC903-Induced Atopic Dermatitis Mice

“…Consistent with the vast majority of recent research reports, NLRP3 expression is up-regulated in AD, and repressing NLRP3 activation can mitigate AD. 58,[60][61][62][63][64] A previous study has also indicated that one of the possible anti-inflammatory mechanisms of CQ is repression of the NLRP3 inflammasome activity. 34 The antimalarial CQ reduces LPS-induced activation of the NLRP3 inflammasomes, thereby conferring protection against murine endotoxic shock.…”

An Overview on Atopic Dermatitis, Oxidative Stress, and Psychological Stress: Possible Role of Nutraceuticals as an Additional Therapeutic Strategy

“…The chlorogenic acid and caffeic acid present in Coffea arabica extract (CAE) have been shown to have antioxidant activity both in vitro and in vivo. In vitro CAE treatment results in reduced levels of IFN-γ/TNF-α-induced intracellular ROS and increased levels of antioxidant enzymes such as CAT, thereby improving the balance between oxidant and antioxidant factors at the skin level [100]. In vivo, caffeic acid not only improves AD lesions, by reducing TEWL and restoring the skin barrier and IVL, FLG, and LOR, but also gives the best results in terms of scavenging free radicals and inhibiting lipid peroxidation [101].…”