Coffea Canephora Extract as Antibacterial Agent against Escherichia coli

Abstract

Introduction: This research is investigate antibacterial activity of Coffea canephora extract against Gram-Positive Escherichia coli.Methods: An experimental laboratory with post-test only group design, investigating antibacterial activity of Coffea canephora extract in concentrations 4%, 16%, 25%, 33%, 39% and 44% were observed and measured mean diameter inhibitory zone then analysed by statistical and sensitivity test. Sensitivity criteria according standard of Clinical and Laboratory Standarts Institiute (CLSI). Statistical test using Kruskal-Wallis and Mann-Whitney Test. Conclusion:In The Coffea canephora extract showed lowest concentration to inhibit Escherichia coli was 33% (Mean : 9mm) although categorized as resistant. Greatest concentration 44% (Mean: 13.75mm) was categorized as intermediate. Statistical analysis using Kruskal-Wallis showed significant difference in all concentration and Mann-Whitney showed significant mean diameter inhibitory zone in concentration 33%, 39% and 44%. In this study we showed Coffea canephora extract perform antibacterial activity against Escherichia coli although in current study no sensitive concentration found. Increase concentration correlated with increase mean diameter inhibitory zone which indicated antibacterial activity.