Coffee biotechnology

Santos-Briones, César De los; Hernández‐Sotomayor, S.M. Teresa

doi:10.1590/s1677-04202006000100015

Cited by 24 publications

(14 citation statements)

References 54 publications

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“…The low concentration of endophytes in the seeds [25,30] and the development of new embryos by adventive embryogenesis increases the chance of developing Burkholderia -free plants. In Coffea arabica (Rubiaceae), it occurs uncommonly in vitro , but it is mostly induced during plant cultivation with plant hormones (2,4-dichlorophenoxyacetic acid or naphthalene acetic acid) [51,52]. We observed adventive embryogenesis in 10–30% of the developing embryos after EC in the absence of plant hormones.…”

Section: Discussionmentioning

confidence: 87%

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

et al. 2019

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Background & aims The bacterial leaf nodule symbiosis is an interaction where bacteria are housed in specialised structures in the leaves of their plant host. In the Rubiaceae plant family, host plants interact with Burkholderia bacteria. This interaction might play a role in the host plant defence system. It is unique due to its high specificity; the vertical transmission of the endophyte to the next generation of the host plant; and its supposedly obligatory character. Although previous attempts have been made to investigate this obligatory character by developing Burkholderia -free plants, none have succeeded and nodulating plants were still produced. In order to investigate the obligatory character of this endosymbiosis, our aims were to develop Burkholderia- free Psychotria umbellata plants and to investigate the effect of the absence of the endophytes on the host in a controlled environment. Methods The Burkholderia- free plants were obtained via embryo culture, a plant cultivation technique. In order to analyse the endophyte-free status, we screened the plants morphologically, microscopically and molecularly over a period of three years. To characterise the phenotype and growth of the in vitro aposymbiotic plants, we compared the growth of the Burkholderia -free plants to the nodulating plants under the same in vitro conditions. Key results All the developed plants were Burkholderia -free and survived in a sterile in vitro environment. The growth analysis showed that plants without endophytes had a slower development. Conclusions Embryo culture is a cultivation technique with a high success rate for the development of Burkholderia -free plants of P . umbellata . The increased growth rate in vitro when the specific endophyte is present cannot be explained by possible benefits put forward in previous studies. This might indicate that the benefits of the endosymbiosis are not yet completely understood.

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Section: Discussionmentioning

confidence: 87%

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

et al. 2019

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“…Pada tanaman kopi, metode embriogenesis somatik telah diteliti dan memberikan alternatif perbanyakan varietas unggul baru hasil persilangan maupun hasil rekayasa genetik (Gatica, Arrieta, Espinoza, & Rica, 2009). Metode tersebut dapat digunakan untuk memproduksi benih kopi yang relatif seragam dalam skala besar dengan waktu yang lebih singkat dan bebas hama penyakit (Santos-briones & Hernández-sotomayor, 2006;Georget et al, 2017).…”

Section: Pendahuluanunclassified

Peningkatan Induksi Kalus Embriogenik dan Konversi Embrio Somatik Kopi Robusta Klon BP 308

Ibrahim

Hartati

2017

J. tanam. industri dan penyegar

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Robusta coffee (Coffea canephora) is a cross-pollinated plant, therefore vegetative propagation is necessary to ensure identical traits with parents, such as tissue culture techniques through somatic embryo. The study aimed to find the effect of plant growth regulator 2.4-D and thidiazuron in inducing embryogenic callus, by adding incision area on leaf explant, and to evaluate addition of GA3 in increasing somatic embryo conversion. The study was conducted from December 2014 to June 2016 in the Tissue Culture Laboratory, IAARD, Bogor. The research consisted of 2 stages. Stage 1 used a complete randomized design of 2 factors; the first factor was a combination of plant growth regulator 2.4-D (1.0 and 2.0 mg/l) and thidiazuron (1.0; 3.0; and 5.0 mg/l), the second factor was leaf incision (slashed and unslashed). Stage 2 used a complete randomized design, with GA3 treatment at different concentrations (0.0; 0.5; and 1.0 mg/l). Observed variables were percentage of callus formation, fresh weight of callus, number of torpedoes, number of somatic embryos at cotyledon stage, and number of germinated embryo. The results showed growth regulatory treatments influenced the percentage of embryogenic callus formation and fresh weight of callus. Extra incision on leaf showed no effect in embryogenic callus induction. Embryogenic callus inducted using 2.4-D 1.0 mg/l + thidiazuron 5.0 mg/l medium which then regenerated in ½ MS medium added with kinetin 2 mg/l exhibited the highest number of germination. Adding GA3 0.1 mg/l in regeneration medium is recommended to increase somatic embryos of Robusta coffee BP 308 clone.

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“…Coffee quality, which determines its marketability and price, is a complex trait resulting from multifactorial determinants such as plant genetics, pedoclimatic conditions, and pre-and postharvest treatments like roasting and brewing (Martin et al 1999;Montavon et al 2003;De Los Santos-Briones and Hernández-Sotomayor 2006;Leroy et al 2006). Although most of the studies directed at improving coffee quality have focused on physiological and agronomic aspects of the coffee plant (Montavon et al 2003;Leroy et al 2006), the microbiota associated with coffee plants may play a critical role in the final expression of coffee quality (Pasin et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Endophytic microbial diversity in coffee cherries ofCoffea arabicafrom southeastern Brazil

et al. 2013

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The microbiota associated with coffee plants may play a critical role in the final expression of coffee quality. However, the microbial diversity in coffee cherries is still poorly characterized. Here, we investigated the endophytic diversity in cherries of Coffea arabica by using culture-independent approaches to identify the associated microbes, ultimately to better understand their ecology and potential role in determining coffee quality. Group-specific 16S rRNA and 26S rRNA genes polymerase chain reaction - denaturing gradient gel electrophoresis and clone library sequencing showed that the endophytic community is composed of members of the 3 domains of life. Bacterial sequences showing high similarity with cultured and uncultured bacteria belonged to the Betaproteobacteria, Gammaproteobacteria, and Firmicutes phyla. Phylogenetic analyses of cloned sequences from Firmicutes revealed that most sequences fell into 3 major genera: Bacillus, Staphylococcus, and Paenibacillus. Archaeal sequences revealed the presence of operational taxonomic units belonging to Euryarchaeota and Crenarchaeota phyla. Sequences from endophytic yeast were not recovered, but various distinct sequences showing high identity with filamentous fungi were found. There was no obvious correlation between the microbial composition and cultivar or geographic location of the coffee plant. To the best of our knowledge, this is the first report demonstrating internal tissue colonization of plant fruits by members of the Archaea domain. The finding of archaeal small-subunit rRNA in coffee cherries, although not sufficient to indicate their role as active endophytes, certainly expands our perspectives toward considering members of this domain as potential endophytic microbes.

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Coffee biotechnology

Cited by 24 publications

References 54 publications

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

Peningkatan Induksi Kalus Embriogenik dan Konversi Embrio Somatik Kopi Robusta Klon BP 308

Endophytic microbial diversity in coffee cherries ofCoffea arabicafrom southeastern Brazil

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