Coffee Ingestion Suppresses Hyperglycemia in Streptozotocin-Induced Diabetic Mice

Abstract

Summary Coffee consumption reduces the risk of type 2 diabetes in humans, but the mechanism remains unclear. In this study, we investigated the effect of coffee on pancreatic b-cells in the induction of diabetes by streptozotocin (STZ) treatment in mice. We examined the effect of coffee, caffeine, or decaffeinated coffee ingestion on STZ-induced hyperglycemia. After STZ injection in Exp. 1 and 2, serum glucose concentration and water intake in coffee ingestion (Coffee group) tended to be lowered or was significantly lowered compared to those in water ingestion (Water group) instead of coffee. In Exp. 1, the values for water intake and serum glucose concentration in caffeine ingestion (Caffeine group) were similar to those in the Water group. In Exp. 2, serum glucose concentrations in the decaffeinated coffee ingestion (Decaf group) tended to be lower than those in the Water group. Pancreatic insulin contents tended to be higher in the Coffee and Decaf groups than in the Water group (Exp. 1 and 2). In Exp. 3, subsequently, we showed that coffee ingestion also suppressed the deterioration of hyperglycemia in diabetic mice which had been already injected with STZ. This study showed that coffee ingestion prevented the development of STZ-induced diabetes and suppressed hyperglycemia in STZ-diabetic mice. Caffeine or decaffeinated coffee ingestion did not significantly suppress STZ-induced hyperglycemia. These results suggest that the combination of caffeine and other components of decaffeinated coffee are needed for the preventive effect on pancreatic b-cell destruction. Coffee ingestion may contribute to the maintenance of pancreatic insulin contents. Key Words streptozotocin, diabetes, hyperglycemia, coffee, caffeine In 2015, 415 million people worldwide were estimated to be affected by type 2 diabetes (1). The International Diabetes Federation estimates that this will rise to 642 million individuals by the year 2040, and warns that the prevalence of type 2 diabetes will increase. These increases are caused by changes in environmental factors such as quantity and quality of food and exercise, and therefore modification of environmental factors can contribute to the prevention of type 2 diabetes.In humans, many studies have revealed an association between coffee consumption and decreased risk of type 2 diabetes. A meta-analysis of 28 studies published between 1966 and 2013 showed that daily consumption of six cups of coffee was associated with a 33% lower risk of diabetes, and that coffee consumption dose-dependently reduced the risk of type 2 diabetes (2). In addition, the reduction in type 2 diabetes risk was observed with both, caffeinated and decaffeinated coffee. However, the mechanism of the anti-diabetic effect of coffee remains unclear.Previously we reported that coffee had an anti-diabetic effect in both spontaneous and diet-induced type 2 diabetes in animal models (3, 4). In KK-A y mice, which spontaneously develop type 2 diabetes, coffee ingestion suppressed hyperglycemia, hyperinsulinemia, hyperlipidemia...

Lactam ent-Kaurane Diterpene: A New Class of Diterpenoids Present in Roasted Beans of Coffea arabica

“…Extensive epidemiological studies have shown that consumption of coffee is conducive for preventing diabetes, and studies have shown that caffeine, chlorogenic acid, and trigonelline in coffee have been shown to exert certain antidiabetic activities through different mechanisms. − However, little research has been done on whether the trace components in coffee exert antidiabetic activity. α-Glucosidase plays a key role in the metabolism of carbohydrates in the human body and is considered to be an important target for the control of diabetes.…”

PENGARUH PEMBERIAN SEDUHAN KOPI ROBUSTA (Coffea canephora) TERHADAP JUMLAH SEL BETA PANKREAS (Studi Eksperimen Pada Tikus Putih (Rattus Norvegicus) Yang Diinduksi Streptozotocin)

“…Seperti yang disebutkan oleh (Anwari, 2021) kopi mempunyai kandungan asam klorogenat bisa melakukan penurunan kadar glukosa darah yakni dengan melakukan pencegahan timbulnya sel beta pankreas yang gagal dalam melakukan produksi insulin. Penelitian yang dilaksanakan (Misato et al, 2017) menyebutkan yakni asam klorogenat bekerja dengan cara melakukan penghambatan kegitan translokasa glukosa-6 fosfat. Terhalangnya tahapan-tahapan ini mengakibatkan glukosa yang terserap pada saluran gastrointestinal menjadi delay yang lalu akan memberikan rangsangan sekresi dari glucagon-like peptide 1 (GLP-1).…”