The aim of this study is to investigate the relationship between life orientation and cognitive emotion regulation with job stress. On this basis, a sample of 100 employees working in Guilan Education Office was used and research hypotheses were examined by job stress, life orientation and cognitive emotion regulation questionnaires. The results of this study indicate that job stress has a significant relationship with life orientation (−0.32), self-blame (0.25), rumination (−0.36), positive refocusing (0.25), positive refocusing on program (−0.21), positive reappraisal (−0.28), catastrophizing (−0.39) and other blames (−0.25). Furthermore, life orientation and cognitive regulation could explain 22% variances in respondents' job stress. This finding indicates that more positive life orientation and subsequent decrease of emotion-regulation-related disorders will lead to less job stress.