Cognitive improvement effect of nervonic acid and essential fatty acids on rats ingesting<i>Acer truncatum Bunge</i>seed oil revealed by lipidomics approach

Song, Wangting; Zhang, Ke; Xue, Teng; Han, Jiarui; Ding, Chunguang; Peng, Fangda; Lin, Feng; Li, Jiujun; Agassi, Sze Fat Tin; Gan, Jianwen; Chen, Xianyang

doi:10.1039/d1fo03671h

Cited by 26 publications

(32 citation statements)

References 68 publications

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“…As reported in Table 1, we identified and quantified 18 FAs in the ASO, and noticed that 88.96% of the FAs were unsaturated, including 58.47% monounsaturated fatty acids (MUFA). In accordance with earlier research, 16,17,21 our findings showed that the top five FAs in the ASO were linoleic acid (28.21%), oleic acid (21.83%), lysergic acid (19.99%), cis , cis -11-eicosenoic acid (8.59%), and NA (8%). Also, the content of ω-3, 6, 9 in the ASO were ω-3 FAs (1.48%), ω-6 FAs (29.01%) and ω-9 FAs (58.41%), respectively.…”

Section: Resultssupporting

confidence: 92%

Lipidomics reveal the cognitive improvement effects of Acer truncatum Bunge seed oil on hypoxic–ischemic encephalopathy rats

Chen¹,

Song²,

Song³

et al. 2023

Food Funct.

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Section: Resultssupporting

confidence: 92%

Lipidomics reveal the cognitive improvement effects of Acer truncatum Bunge seed oil on hypoxic–ischemic encephalopathy rats

Chen¹,

Song²,

Song³

et al. 2023

Food Funct.

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“…More and more studies show that nervonic acid benefits neurological health (Wadhwani et al, 2011; Li et al, 2019) and has therapeutic potential for adrenoleukodystrophy and multiple sclerosis (Lewkowicz et al, 2019; Terluk et al, 2022). Moreover, nervonic acid levels in specific tissues are associated with Alzheimer’s disease (Astarita et al, 2011), Parkinson’s disease (Hu et al, 2021), cognition (Song et al, 2022), mood symptoms (Kageyama et al, 2018; Kageyama et al, 2021), cardiovascular death (Delgado et al, 2017) and even obesity (Keppley et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

High-level production of nervonic acid in the oleaginous yeastYarrowia lipolyticaby systematic metabolic engineering

Shi

Shen

et al. 2023

Preprint

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Brain and neurological diseases are influencing more than one billion world's people. Nervonic acid (cis-15-tetracosenoic acid, C24:1 Δ15) benefits the treatment of neurological diseases and the health of brain. Currently, the sources of nervonic acid are limited to the seeds of a couple of plants. In this study, we employed the oleaginous yeast Yarrowia lipolytica to overproduce nervonic acid oil by systematic metabolic engineering. First, engineering the fatty acid elongation (FAE) pathway by expressing a heterologous β-ketoacyl-CoA synthase gene CgKCS enabled the production of nervonic acid in Y. lipolytica. Second, modulation of endogenous pathways by expressing a C16:0-acyl-CoA preferred fatty acid elongase gELOVL6 together with a C18:0-acyl-CoA preferred fatty acid desaturase MaOLE2 increased the content of nervonic acid in total fatty acids (TFA). Third, iterative expression of CgKCS, gELOVL6 and MaOLE2 at the genomic loci of rDNA, FAD2, TGL4, GSY1 and SNF1 dramatically improved the production of nervonic acid. Fourth, the biosynthesis of both nervonic acid and lipids were further enhanced by expression of the MaOLE2-CgKCS fusion protein and glycerol-3-phosphate acyltransferases (GPAT) and diacylglycerol acyltransferases (DGAT) from Malania oleifera in the endoplasmic reticulum (ER) membrane. Fifth, an ER structure regulator YlINO2 was identified in Y. lipolytica and the overexpression of YlINO2 led to a 39.3% increase in lipid production. Next, pilot-scale fermentation in 50-L reactor using the strain YLNA9 exhibited a lipid titer of 96.7 g/L and a nervonic acid titer of 17.3 g/L, the highest reported titer to date for de novo nervonic acid production. We also found that disruption of the AMP-activated S/T protein kinase SNF1 increased the ratio of nervonic acid (C24:1) to lignoceric acid (C24:0) by 61.6% and a ratio of 3.5:1 (nervonic acid to lignoceric acid) was achieved in the strain YLNA10. Finally, a proof-of-concept purification and separation of nervonic acid were performed and the purity of it reached 98.7%. This study suggested that oleaginous yeasts are attractive hosts for the cost-efficient production of nervonic acid and possibly other very long-chain fatty acids (VLCFAs).

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“…Nervonic acid is crucial for nerve myelin synthesis, which is one of the fundamental components of the nerve and brain ( Poulos, 1995 ; Merrill et al, 1997 ; Martínez and Mougan, 1998 ). It has been found that nervonic acid is essential for developing the human nervous system and can be used to prevent and treat various neurodegenerative diseases ( Kageyama et al, 2021 ; Song et al, 2022 ; Terluk et al, 2022 ). However, long-term exposure to excessive levels of erucic acid can reduce the β-oxidation of erucic acid in animal myocardia, leading to lipid deposition and tissue damage ( Bremer and Norum, 1982 ).…”

Section: Introductionmentioning

confidence: 99%

Genomic and transcriptomic analyses provide insights into valuable fatty acid biosynthesis and environmental adaptation of yellowhorn

Qiang

Liu²,

Fang³

et al. 2022

Front. Plant Sci.

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Yellowhorn (Xanthoceras sorbifolium) is an oil-bearing tree species growing naturally in poor soil. The kernel of yellowhorn contains valuable fatty acids like nervonic acid. However, the genetic basis underlying the biosynthesis of valued fatty acids and adaptation to harsh environments is mainly unexplored in yellowhorn. Here, we presented a haplotype-resolved chromosome-scale genome assembly of yellowhorn with the size of 490.44 Mb containing scaffold N50 of 34.27 Mb. Comparative genomics, in combination with transcriptome profiling analyses, showed that expansion of gene families like long-chain acyl-CoA synthetase and ankyrins contribute to yellowhorn fatty acid biosynthesis and defense against abiotic stresses, respectively. By integrating genomic and transcriptomic data of yellowhorn, we found that the transcription of 3-ketoacyl-CoA synthase gene XS04G00959 was consistent with the accumulation of nervonic and erucic acid biosynthesis, suggesting its critical regulatory roles in their biosynthesis. Collectively, these results enhance our understanding of the genetic basis underlying the biosynthesis of valuable fatty acids and adaptation to harsh environments in yellowhorn and provide foundations for its genetic improvement.

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Cognitive improvement effect of nervonic acid and essential fatty acids on rats ingestingAcer truncatum Bungeseed oil revealed by lipidomics approach

Abstract: The oil of Acer truncatum Bunge seed (ASO) is rich in ω-9 (53.93%) and ω-6 (30.7%) fatty acids (FAs), and characterized by 3-7% nervonic acid (NA, C24:1ω-9). Evidence suggests that...

Cited by 26 publications

References 68 publications

Lipidomics reveal the cognitive improvement effects of Acer truncatum Bunge seed oil on hypoxic–ischemic encephalopathy rats

Lipidomics reveal the cognitive improvement effects of Acer truncatum Bunge seed oil on hypoxic–ischemic encephalopathy rats

High-level production of nervonic acid in the oleaginous yeastYarrowia lipolyticaby systematic metabolic engineering

Genomic and transcriptomic analyses provide insights into valuable fatty acid biosynthesis and environmental adaptation of yellowhorn

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