Coherence‐Tailored Multiwavelength High‐Speed Quantitative Phase Imaging with a High Phase Stability via a Frequency Comb

Boonruangkan, Jeeranan; Farrokhi, Hamid; Rohith, Thazhe Madam; Toh, Hui Ting; Mishra, Awadhesh Kumar; Yoon, Ho Sup; Kwok, S.P.Y.; Carney, Thomas J.; Kim, Seung-Woo; Kim, Young‐Jin

doi:10.1002/adpr.202000088

Cited by 3 publications

(2 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The QPI setup in our study is shown in Figure 2a [17]. The configuration included a frequency comb (FC-1500-250-WC, M-VIS, Menlo Systems, Planegg, Germany) that was used as a speckle-tailored coherent light source for QPI to study the dynamic of cellular changes such as osmotic swelling [18].…”

Section: Qpi Setupmentioning

confidence: 99%

Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates

et al. 2021

Self Cite

View full text Add to dashboard Cite

Decellularization is the process of removing cellular components from native tissues or organs to obtain an acellular, collagenous scaffold for use in tissue engineering and organ regeneration. Surfactants are widely used to produce acellular scaffolds for clinical applications. However, cell–surfactants interactions have not been studied in depth. Cell-surfactant interaction was studied in a time-lapsed manner using sodium dodecyl sulfate (SDS) solution (surfactant) on adherent L929 fibroblasts as a test solution, phosphate-buffered saline (PBS) solution as control solution (isotonic), and deionized water as positive test solution (hypotonic), respectively. The QPI results show changes in the relative height and cross-sectional area of the cells, with various test solutions and exposure times. In particular, it was observed that the removal of the cell with SDS involved the disruption of the cellular membrane and detachment of the cell contents from the adhering surface. This study demonstrated the feasibility of using the QPI technique to understand the decellularization process.

show abstract

Section: Qpi Setupmentioning

confidence: 99%

Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…[1][2][3][4][5] Due to its high stabilities in the frequency and time domains, the frequency comb has been widely used in various applications, for example, spectroscopy, imaging, communications, and so on. [6][7][8][9][10] Among them, the dual-comb technique is a direct application of the frequency comb and shows prominent advantages in the fast spectroscopy without a need of any moving part. In comparison, traditional infrared and terahertz spectrometers (for instance, the Fouriertransform infrared (FTIR) and the time domain spectrometers) need mechanical scanning mirrors for obtaining a complete spectrum.…”

Section: Introductionmentioning

confidence: 99%

Broadband Terahertz Quantum Cascade Laser Dual‐Comb Sources under Off‐Resonant Microwave Injection

Liao

Zhou

et al. 2022

Advanced Photonics Research

View full text Add to dashboard Cite

Broadband dual‐comb spectroscopy has attracted increasing interests due to its unique advantages in high spectral resolution, fast detection, and so on. Although the dual‐comb technique is relatively mature in the infrared wavelengths, it is, currently, not commercially capable of practical applications in the terahertz regime due to the lack of high‐performance broadband terahertz dual‐comb sources. In the terahertz frequency range, the electrically pumped quantum cascade laser (QCL) is a suitable candidate for the dual‐comb operation. Although the resonant microwave injection locking is widely used to broaden the emission spectra of terahertz QCLs, it is challenging to be employed to obtain broadband dual‐comb sources that can fully exploit the laser gain bandwidth due to the large phase noise induced by the resonant injection and nonideal microwave circuits. Herein, an off‐resonant microwave injection to significantly broaden the dual‐comb bandwidth of a terahertz QCL dual‐comb source is used. The measured optical dual‐comb bandwidth is broadened from 147 GHz in free running to >450 GHz under the off‐resonant injection. By performing a numerical analysis based on a rate equation model, the broadband dual‐comb operation under the off‐resonant microwave injection can result from a wider lasing bandwidth and a higher degree of phase matching.

show abstract

Compact Linnik‐type hyperspectral quantitative phase microscope for advanced classification of cellular components

Joshi,

Singh,

Butola

et al. 2024

Journal of Biophotonics

View full text Add to dashboard Cite

Hyperspectral quantitative phase microscopy (HS‐QPM) involves the acquisition of phase images across narrow spectral bands, which enables wavelength‐dependent study of different biological samples. In the present work, a compact Linnik‐type HS‐QPM system is developed to reduce the instability and complexity associated with conventional HS‐QPM techniques. The use of a single objective lens for both reference and sample arms makes the setup compact. The capabilities of the system are demonstrated by evaluating the HS phase map of both industrial and biological specimens. Phase maps of exfoliated cheek cells at different wavelengths are stacked to form a HS phase cube, adding spectral dimensionality to spatial phase distribution. Analysis of wavelength response of different cellular components are performed using principal component analysis to identify dominant spectral features present in the HS phase dataset. Findings of the study emphasize on the efficiency and effectiveness of HS‐QPM for advancing cellular characterization in biomedical research and clinical applications.

show abstract

Coherence‐Tailored Multiwavelength High‐Speed Quantitative Phase Imaging with a High Phase Stability via a Frequency Comb

Cited by 3 publications

References 51 publications

Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates

Quantitative Phase Imaging to Study the Effect of Sodium Dodecyl Surfactant on Adherent L929 Fibroblasts on Tissue Culture Plates

Broadband Terahertz Quantum Cascade Laser Dual‐Comb Sources under Off‐Resonant Microwave Injection

Compact Linnik‐type hyperspectral quantitative phase microscope for advanced classification of cellular components

Contact Info

Product

Resources

About