Coherency image analysis to quantify collagen architecture: implications in scar assessment

Clemons, Tristan D.; Bradshaw, Michael; Toshniwal, Priyanka; Chaudhari, Nutan; Stevenson, Andrew; Lynch, Jessica M.; Fear, Mark W.; Wood, Fiona; Iyer, K. Swaminathan

doi:10.1039/c7ra12693j

Cited by 83 publications

(69 citation statements)

References 44 publications

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“…OrientationJ plugin was used to quantify the coherency parameter by choosing a specific sequence of ROIs in confocal acquisitions, based on the measure of the structure tensors in a local neighborhood. At the same time, the software calculated the value of orientation and coherency that represented the degree to which the actin fibers were oriented: more disordered fibers have values near 0, whereas perfectly aligned ones show coherency value of about 1 [34]. Integrated density was also calculated by the sum of the pixels values in the ROIs on confocal acquisitions in order to quantify the amount of actin fibers in cells.…”

Section: Methodsmentioning

confidence: 99%

Tailoring Cell Morphomechanical Perturbations Through Metal Oxide Nanoparticles

et al. 2019

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The nowadays growing use of nanoparticles (NPs) in commercial products does not match a comprehensive understanding of their potential harmfulness. More in vitro investigations are required to address how the physicochemical properties of NPs guide their engulfment within cells and their intracellular trafficking, fate, and toxicity. These nano-bio interactions have not been extensively addressed yet, especially from a mechanical viewpoint. Cell mechanic is a critical indicator of cell health because it regulates processes like cell migration, tissue integrity, and differentiation via cytoskeleton rearrangements. Here, we investigated in vitro the elasticity perturbation of Caco-2 and A549 cell lines, in terms of Young’s modulus modification induced by SiO 2 NP S and TiO 2 NP S . TiO 2 NPs demonstrated stronger effects on cell elasticity compared to SiO 2 NPs, as they induced significant morphological and morphometric changes in actin network. TiO 2 NP S increased the elasticity in Caco-2 cells, while opposite effects have been observed on A549 cells. These results demonstrate the existence of a correlation between the alteration of cell elasticity and NPs toxicity that depends, in turn, on the NPs physicochemical properties and the specific cell tested.

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Section: Methodsmentioning

confidence: 99%

Tailoring Cell Morphomechanical Perturbations Through Metal Oxide Nanoparticles

et al. 2019

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“…In order to eliminate the noise resulting from the presence of biocarbon particles and the background of the image itself, image processing was applied, and a binary object mask was created. This procedure was developed to assess the orientation of tissue structures such as collagen fibers [48][49][50], while it is often used for evaluation of the fiber arrangement in polymer composites [51][52][53], which is also the subject of the presented research.…”

Section: Characterizationmentioning

confidence: 99%

The Influence of the Hybridization Process on the Mechanical and Thermal Properties of Polyoxymethylene (POM) Composites with the Use of a Novel Sustainable Reinforcing System Based on Biocarbon and Basalt Fiber (BC/BF)

et al. 2020

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The presented work focuses on the assessment of the material performance of polyoxymethylene (POM)-based composites reinforced with the use of a biocarbon/basalt fiber system (BC/BF). The use of BC particles was aimed at eliminating mineral fillers (chalk, talc) by using fully biobased material, while basalt fibers can be considered an alternative to glass fibers (GF). All materials were prepared with the same 20% filler content, the differences concerned the (BC/BF) % ratio. Hybrid samples with (25/75), (50/50), and (75/25) ratios were prepared. Additionally, reference samples were also prepared (POM BC20% and POM BF20%.). Samples prepared by the injection molding technique were subjected to a detailed analysis of mechanical properties (static tensile and Charpy impact tests), thermomechanical characteristics (dynamic mechanical thermal analysis—DMTA, heat deflection temperature - HDT), and thermal and rheological properties (DSC, rotational rheometer tests). In order to assess fiber distribution within the material structure, the samples were scanned by a microtomography method (μCT). The addition of even a significant amount of BC particles did not cause excessive material brittleness, while the elongation and impact strength of all hybrid samples were very similar to the reference POM BF20% sample. The tensile modulus and strength values appear to be strictly dependent on the increasing BF fiber content. Thermomechanical analysis (DMTA, HDT) showed very similar heat resistance for all hybrid samples; the results did not differ from the values for the POM BF20 sample.

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“…Therefore, while this could indicate a decrease in the intrinsic stiffness of collagen fibers, it does not eliminate the possibility of tissue-level structure/architecture affecting the observed behavior (Hill et al, 2014). For instance, reduced collagen fiber coherency was detected at the endocardial levels ( Fig 4h ), indicating a more sparse and isotropic distribution of collagen fibers in the aging group (Clemons et al, 2018). This has the potential to affect the load transfer mechanism of endocardial collagen fibers, contributing to reduced effective modulus at the tissue level.…”

Section: Discussionmentioning

confidence: 97%

Effects of Healthy Aging on Right Ventricular Structure and Biomechanical Properties

Kia

Shen

Bachman

et al. 2020

Preprint

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Healthy aging has been associated with alterations in pulmonary vasculature and right ventricular (RV) hemodynamics, potentially leading to RV remodeling. Despite the current evidence suggesting an association between aging and alterations in RV function and higher prevalence of pulmonary hypertension in the elderly, limited data exist on age-related differences in RV structure and biomechanics. In this work we report our preliminary findings on the effects of healthy aging on RV structure, function, and biomechanical properties. Hemodynamic measurements, biaxial mechanical testing, constitutive modeling, and quantitative histological analysis were employed to study two groups of Sprague-Dawley rats: control (11 weeks) and aging (80 weeks).Aging was associated with increases in RV peak pressures (≈↑17%, p=0.017), RV contractility (≈↑52%, p= 0.004), and RV wall thickness (≈↑34%, p=0.002). Longitudinal realignment of RV collagen (16.4°, p=0.013) and myofibers (14.6°, p=0.017) were observed with aging, accompanied by transmural cardiomyocyte loss and fibrosis. A bimodal alteration in biomechanical properties was noted, resulting in increased myofiber stiffness (≈↑158%, p=0.0006) and decreased effective collagen fiber stiffness (≈↓67%, p=0.031).Our results demonstrate the potential of healthy aging to modulate RV remodeling via increased peak pressures, cardiomyocyte loss, fiber reorientation, and altered collagen/myofiber stiffness. Some similarities were observed between aging-induced remodeling patterns and those of RV remodeling in pressure overload.

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Coherency image analysis to quantify collagen architecture: implications in scar assessment

Abstract: A novel technique for the fast and robust quantification of collagen architecture following scarring.

Cited by 83 publications

References 44 publications

Tailoring Cell Morphomechanical Perturbations Through Metal Oxide Nanoparticles

Tailoring Cell Morphomechanical Perturbations Through Metal Oxide Nanoparticles

The Influence of the Hybridization Process on the Mechanical and Thermal Properties of Polyoxymethylene (POM) Composites with the Use of a Novel Sustainable Reinforcing System Based on Biocarbon and Basalt Fiber (BC/BF)

Effects of Healthy Aging on Right Ventricular Structure and Biomechanical Properties

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