Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system

Bégin, Steve; Burgoyne, Bryan; Mercier, Vincent; Villeneuve, A.; Vallée, Réal; Côté, Daniel

doi:10.1364/boe.2.001296

Cited by 93 publications

(79 citation statements)

References 26 publications

(26 reference statements)

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“…Here we introduce narrowband hyperspectral SFG microscopy as an attractive option for spectral mapping of three-dimensional biological samples. This approach is very similar to picosecond hyperspectral CARS [41][42][43] and SRS [44][45][46] microscopy, where the wavelength of one of the incident beams is systematically varied in between images. At 1 frame/s, the acquisition time of a hyperspectral datastack is limited by the time it takes to change the wavelength.…”

Section: Discussionmentioning

confidence: 99%

Hyperspectral imaging with laser-scanning sum-frequency generation microscopy

Hanninen¹,

Shu²,

Potma³

2017

Biomed. Opt. Express

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Vibrationally sensitive sum-frequency generation (SFG) microscopy is a chemically selective imaging technique sensitive to non-centrosymmetric molecular arrangements in biological samples. The routine use of SFG microscopy has been hampered by the difficulty of integrating the required mid-infrared excitation light into a conventional, laser-scanning nonlinear optical (NLO) microscope. In this work, we describe minor modifications to a regular laserscanning microscope to accommodate SFG microscopy as an imaging modality. We achieve vibrationally sensitive SFG imaging of biological samples with sub-µm resolution at image acquisition rates of 1 frame/s, almost two orders of magnitude faster than attained with previous point-scanning SFG microscopes. Using the fast scanning capability, we demonstrate hyperspectral SFG imaging in the CH-stretching vibrational range and point out its use in the study of molecular orientation and arrangement in biologically relevant samples. We also show multimodal imaging by combining SFG microscopy with second-harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) on the same imaging platfrom. This development underlines that SFG microscopy is a unique modality with a spatial resolution and image acquisition time comparable to that of other NLO imaging techniques, making point-scanning SFG microscopy a valuable member of the NLO imaging family.

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Section: Discussionmentioning

confidence: 99%

Hyperspectral imaging with laser-scanning sum-frequency generation microscopy

Hanninen¹,

Shu²,

Potma³

2017

Biomed. Opt. Express

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“…CARS [164], and its hyperspectral version [165], are other promising non-linear techniques for histology, which reveal a high density of either a single or multiple molecular vibrations inside a sample. Interestingly, CARS is one of the only techniques that is both label-free, and specific at the molecular level, as vibration spectra of given structures can be measured, and computationally extracted [166].…”

Section: Comparison With Other Popular Novel Microscopiesmentioning

confidence: 99%

Full-Field Optical Coherence Tomography as a Diagnosis Tool: Recent Progress with Multimodal Imaging

et al. 2017

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Full-field optical coherence tomography (FF-OCT) is a variant of OCT that is able to register 2D en face views of scattering samples at a given depth. Thanks to its superior resolution, it can quickly reveal information similar to histology without the need to physically section the sample. Sensitivity and specificity levels of diagnosis performed with FF-OCT are 80% to 95% of the equivalent histological diagnosis performances and could therefore benefit from improvement. Therefore, multimodal systems have been designed to increase the diagnostic performance of FF-OCT. In this paper, we will discuss which contrasts can be measured with such multimodal systems in the context of ex vivo biological tissue examination. We will particularly emphasize three multimodal combinations to measure the tissue mechanics, dynamics, and molecular content respectively.

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“…A rapidly tunable fiberlasers can be used, which allows to access any wavelength within a tuning range of 250 cm À1 at a rate of 10 kHz for fast acquisition of hyperspectral image data [60]. However, the commonly used 80 MHz repetition rate lasers do not simultaneously provide sufficient peak power and spectral pulse width matching the requirements of SHG, TPEF and CRS.…”

Section: Technologymentioning

confidence: 99%

Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

Meyer

Schmitt

Dietzek

et al. 2013

Journal of Biophotonics

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Journal of BIOPHOTONICSMultimodal nonlinear microscopy has matured during the past decades to one of the key imaging modalities in life science and biomedicine due to its unique capabilities of label-free visualization of tissue structure and chemical composition, high depth penetration, intrinsic 3D sectioning, diffraction limited resolution and low phototoxicity. This review briefly summarizes first recent advances in the field regarding the methodology, e.g., contrast mechanisms and signal characteristics used for contrast generation as well as novel image processing approaches. The second part deals with technologic developments emphasizing improvements in penetration depth, imaging speed, spatial resolution and nonlinear labeling strategies. The third part focuses on recent applications in life science fundamental research and biomedical diagnostics as well as future clinical applications.Multimodal nonlinear microscopy of tissue.

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Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system

Cited by 93 publications

References 26 publications

Hyperspectral imaging with laser-scanning sum-frequency generation microscopy

Hyperspectral imaging with laser-scanning sum-frequency generation microscopy

Full-Field Optical Coherence Tomography as a Diagnosis Tool: Recent Progress with Multimodal Imaging

Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

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