Coherent Anti-Stokes Raman Scattering Microscopic Imaging Technique

Jun, 尹君 Yin; Ziyang, 林子扬 Lin; Junle, 屈军乐 Qu; Linyao, 于凌尧 Yu; Xing, 刘星 Liu; Hui, 万辉 Wan; Hanben, 牛憨笨 Niu

doi:10.3788/cjl20093610.2477

Cited by 2 publications

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Introduction on Microscopic Imaging Technique Based on CARS

Xiong¹,

Wang²

2012

2012 International Conference on Biomedical Engineering and Biotechnology

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Coherent Anti-Stokes Raman Scattering (CARS) has been an useful method for microscopic analysis of biological molecule, due to its good directionality, the high scattering intensity and the low background noise. In this paper, we review the development of coherent anti-Stokes Raman scattering (CARS) microscopic imaging in the recent years, introduce the application of CARS technology in microscopic imaging theory as well as the suppression of non-resonant background noise in CARS microscopic imaging technology, and show some typical CARS microscopic imaging systems, advantages and fields of CARS microscopic imaging technique are also conclude at the same time.

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Introduction on Microscopic Imaging Technique Based on CARS

Xiong¹,

Wang²

2012

2012 International Conference on Biomedical Engineering and Biotechnology

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Vector analysis of the coherent anti-Stokes Raman scattering signals generated under the tightly focused condition

Li¹,

Liang²,

Junpeng³

et al. 2014

Acta Phys. Sin.

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In a coherent anti-Stokes Raman scattering (CARS) microscope, when samples with different shapes and dimensions are excitated by collinearly introduced and tightly focused Gaussian beams, the microscopic structure will be determined by the spatial distributions of generated CARS signals. Therefore, we build a theoretical model for CARS signals from spherical sample under the tightly focused condition. The intensity and phase distributions of tightly focused linear polarization Gaussian beams are analyzed with vector wave equations. The vector wave equation of CARS signals is derived from Green's function. The far-field CARS radiation patterns of spherical scatters with different diameters are simulatively calculated. Theoretical analysis and simulative calculation results show that the intensities of forward and backward CARS signals from the small spherical sampler are similar. The images with high contrast can be obtained by backward detection method from an objective with a high numerical aperture. For big spherical samplers, intensities of CARS signals are greatly increased. The emission direction is mainly concentrated in a spatial angle. The forward CARS signals can be effectively collected by an objective with low numerical aperture.

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Coherent Anti-Stokes Raman Scattering Microscopic Imaging Technique

Cited by 2 publications

References 0 publications

Introduction on Microscopic Imaging Technique Based on CARS

Introduction on Microscopic Imaging Technique Based on CARS

Vector analysis of the coherent anti-Stokes Raman scattering signals generated under the tightly focused condition

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