Coherent anti-Stokes Raman scattering microscopy using photonic crystal fiber with two closely lying zero dispersion wavelengths

Murugkar, Sangeeta; Brideau, Craig; Ridsdale, Andrew; Naji, Majid; Stys, Peter K.; Anis, Hanan

doi:10.1364/oe.15.014028

Cited by 53 publications

(43 citation statements)

References 19 publications

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“…A number of groups have published papers that report variations on the laser system described above using 50-200-fsec pulse widths rather than the 2-6-psec pulses (Murugkar et al 2007;Chen et al 2009;Pegoraro et al 2009). Although CRS processes may be excited by femtosecond pulses, this comes at the cost of decreased signal levels, limited tunability, loss of spectral selectivity, and an increased nonresonant background in CARS.…”

Section: Coherent Raman Instrumentationmentioning

confidence: 99%

Coherent Raman Tissue Imaging in the Brain

Saar¹,

Freudiger²,

Xu³

et al. 2014

Cold Spring Harb Protoc

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Imaging in neuroscience has been dramatically impacted by the advent of multiphoton microscopy. Multiphoton-excited fluorescence (MPF) in combination with endogenous fluorophores or labeling by fluorescent molecules has proven to be particularly powerful. However, endogenous fluorescence is limited to relatively few molecular species, and practical labeling schemes do not exist for many classes of molecules. Coherent Raman scattering (CRS) techniques, including coherent anti-Stokes Raman scattering and stimulated Raman scattering, allow imaging without the need for staining or fluorescent labeling. Such label-free imaging is desirable in biomedical research, because labeling often perturbs the function of small metabolite and drug molecules and may be too toxic to use in vivo. CRS techniques have similar imaging parameters to MPF, making use of pulsed near-infrared lasers to deliver high-sensitivity, high spatial resolution in three dimensions and rapid image acquisition. In this introduction, we will discuss the basic principles of CRS imaging, present the instrumentation requirements for high-speed CRS imaging, and show an example of imaging brain tumors and healthy tissue based on their intrinsic vibrational signatures. This discussion is intended to introduce the benefits and tradeoffs associated with different CRS techniques and show one example of the powerful capabilities of label-free chemical imaging.

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Section: Coherent Raman Instrumentationmentioning

confidence: 99%

Coherent Raman Tissue Imaging in the Brain

Saar¹,

Freudiger²,

Xu³

et al. 2014

Cold Spring Harb Protoc

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“…PCF with two zero-dispersion wavelengths (ZDW) can have very low noise levels similar to the oscillators used to pump them (Murugkar et al 2007). This type of two-ZDW PCF is commercially available and targeted specifically for CARS microscopy.…”

Section: Laser Sourcesmentioning

confidence: 99%

Nonlinear optical microscopy in decoding arterial diseases

Ridsdale

Mostaço-Guidolin

et al. 2012

Biophys Rev

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Pathological understanding of arterial diseases is mainly attributable to histological observations based on conventional tissue staining protocols. The emerging development of nonlinear optical microscopy (NLOM), particularly in second-harmonic generation, two-photon excited fluorescence and coherent Raman scattering, provides a new venue to visualize pathological changes in the extracellular matrix caused by atherosclerosis progression. These techniques in general require minimal tissue preparation and offer rapid three-dimensional imaging. The capability of label-free microscopic imaging enables disease impact to be studied directly on the bulk artery tissue, thus minimally perturbing the sample. In this review, we look at recent progress in applications related to arterial disease imaging using various forms of NLOM.

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“…The PCF most typically used for SF-CARS [20] has been selected by some groups for the stability and spectral density of its generated supercontinuum [14,[21][22][23]. Previous work by Hilligsoe et al highlighted this fibre's efficiency in generating supercontinuum beyond its two zero-dispersion wavelengths (ZDWs), which are 775 and 945 nm, using sub-70 fs input pulses [19].…”

Section: Introductionmentioning

confidence: 99%

Spectrally-broad coherent anti-Stokes Raman scattering hyper-microscopy utilizing a Stokes supercontinuum pumped at 800 nm

Porquez

Cole

Tabarangao

et al. 2016

Biomed. Opt. Express

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Abstract:We demonstrate spectral-focusing based coherent anti-Stokes Raman scattering (SF-CARS) hyper-microscopy capable of probing vibrational frequencies from 630 cm 1 to 3250 cm 1 using a single Ti:Sapphire femtosecond laser operating at 800 nm, and a commercially-available supercontinuum-generating fibre module. A broad Stokes supercontinuum with significant spectral power at wavelengths between 800 nm and 940 nm is generated by power tuning the fibre module using atypically long and/or chirped ~200 fs pump pulses, allowing convenient access to lower vibrational frequencies in the fingerprint spectral region. This work significantly reduces the instrumental and technical requirements for multimodal CARS microscopy, while expanding the spectral capabilities of an established approach to SF-CARS.

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Coherent anti-Stokes Raman scattering microscopy using photonic crystal fiber with two closely lying zero dispersion wavelengths

Cited by 53 publications

References 19 publications

Coherent Raman Tissue Imaging in the Brain

Coherent Raman Tissue Imaging in the Brain

Nonlinear optical microscopy in decoding arterial diseases

Spectrally-broad coherent anti-Stokes Raman scattering hyper-microscopy utilizing a Stokes supercontinuum pumped at 800 nm

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