We have measured the spin-forbidden decay rate from (3s3d) 1 D 2 → (3s3p) 3 P 2,1 in 24 Mg atoms trapped in a magneto-optical trap. The total decay rate, summing up both exit channels (3s3p) 3 P 1 and (3s3p) 3 P 2 , yields (196 ± 10) s −1 in excellent agreement with resent relativistic many-body calculations of [S.G. Porsev et al., Phys. Rev. A. 64, 012508 (2001)]. The characterization of this decay channel is important as it may limit the performance of quantum optics experiments carried out with this ladder system as well as two-photon cooling experiments currently explored in several groups.