Cohnella laeviribosi sp. nov., isolated from a volcanic pond

Cho, Eunah; Lee, Jung-Sook; Lee, Keun Chul; Jung, Heung-Chae; Pan, Jae-Gu; Pyun, Yu‐Ryang

doi:10.1099/ijs.0.64844-0

Cited by 41 publications

(20 citation statements)

References 30 publications

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“…Members of the genus Cohnella differ from those of the genus Paenibacillus on the basis of 16S rRNA gene sequence analysis, polar lipid patterns and fatty acid composition (Kämpfer et al, 2006). At present, the genus Cohnella comprises four recognized species, namely Cohnella thermotolerans (the type species), C. hongkongensis (Kämpfer et al, 2006), C. laeviribosi (Cho et al, 2007) and C. phaseoli (García-Fraile et al, 2008). The present study describes two novel Cohnella-like strains.…”

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confidence: 99%

Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils

Kim¹,

Weon²,

Kim³

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Two aerobic, Gram-positive, rod-shaped bacterial strains, 5YN10-14T and GR21-5T, were isolated from the Yongneup wetland and ginseng soil in Korea, respectively. The two strains formed ellipsoidal or oval spores positioned centrally or paracentrally in swollen sporangia. On the basis of 16S rRNA gene sequence analysis, these strains were related to members of the genus Cohnella. 16S rRNA gene sequence similarity between strains 5YN10-14T and GR21-5T was 95.9 %. Strains 5YN10-14T and GR21-5T showed, respectively, 94.3 and 95.2 % 16S rRNA gene sequence similarity to Cohnella thermotolerans CCUG 47242T, 94.6 and 94.4 % to Cohnella hongkongensis HKU3T, 94.7 and 94.7 % to Cohnella laeviribosi RI-39T, and 95.4 and 94.8 % to Cohnella phaseoli GSPC1T. The major fatty acids of strain 5YN10-14T were anteiso-C15 : 0 (51.1 %), iso-C16 : 0 (18.5 %) and C16 : 0 (13.2 %), and the major fatty acids of strain GR21-5T were anteiso-C15 : 0 (48.9 %), iso-C16 : 0 (15.0 %) and iso-C15 : 0 (12.2 %). The two strains contained menaquinone with seven isoprene units (MK-7) as the predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major polar lipids; however, strain 5YN10-14T also contained lysylphosphatidylglycerol as a major polar lipid, whereas strain GR21-5T had an unknown aminophospholipid as another major polar lipid. The DNA G+C contents of strains 5YN10-14T and GR21-5T were 58.8 and 61.3 mol%, respectively. Based on the results of the phylogenetic and phenotypic data presented, it was concluded that the two strains represent two novel species of the genus Cohnella, for which the names Cohnella yongneupensis sp. nov. (type strain 5YN10-14T=KACC 11768T=DSM 18998T) and Cohnella ginsengisoli sp. nov. (type strain GR21-5T=KACC 11771T=DSM 18997T) are proposed.

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confidence: 99%

Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils

Kim¹,

Weon²,

Kim³

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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“…Data for Cohnella were taken from Teng et al (2003), Kämpfer et al (2006) and Cho et al (2007) and data for Thermobacillus were taken from Touzel et al (2000) and Watanabe et al (2007). +, Positive; 2, negative; V, variable; ND, no data available.…”

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confidence: 99%

Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane

Rivas

García‐Fraile

Zurdo-Piñeiro

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A bacterial strain designated GR21 T was isolated from apoplastic fluid of Saccharum officinarum (sugar cane). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family 'Paenibacillaceae', with Paenibacillus as the closest related genus. Within this genus, the closest related species is Paenibacillus xylanilyticus, with 93.4 % similarity to the sequence of the type strain. The isolate has Gram-variable, facultatively anaerobic, rod-shaped cells, motile by polar and subpolar flagella. Round, non-ornamented, central or subterminal spores are formed in unswollen sporangia. The strain is catalase-positive and oxidase-negative on nutrient agar medium. Cellulose and aesculin were hydrolysed, whereas xylan, starch and gelatin were not. Growth was supported by many carbohydrates as carbon sources. Strain GR21 T displayed a lipid profile consisting of diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid, two unknown glycolipids and an unknown phosphoglycolipid. MK-7 was the predominant menaquinone and anteiso-C 15 : 0 was the major fatty acid. The DNA G+C content was 57.8 mol%. Phylogenetic and phenotypic analyses, including assimilation of carbon sources and exoenzyme production commonly used for classification within the family 'Paenibacillaceae', showed that strain GR21 T belongs to a new genus within this family, for which the name Saccharibacillus sacchari gen. nov., sp. nov. is proposed. The type strain of Saccharibacillus sacchari is GR21 T (5LMG 24085 T 5DSM 19268 T ).The family 'Paenibacillaceae' currently contains eight genera of Gram-positive, -variable or -negative sporulating bacilli. The description of the most recently described genus of the family, Cohnella (Kämpfer et al., 2006), was based on its phylogenetic relationship with respect to the remaining genera of the family as well as on its polar lipid profile. Some species of this family, mainly belonging to the genus Paenibacillus, have been isolated from the plant rhizosphere, but only Paenibacillus polymyxa has been reported as a plant endophyte (Cho et al., 2006). In the present work, we describe the isolation and identification of a novel bacterium isolated from apoplastic sap of sugar cane (Saccharum officinarum) in Granada (Spain).For the isolation of strain GR21 T , stem pieces were washed with sterile tap water and soaked in 5 % chloramine T solution for 2 min. After removal of the rind with a sterile sharp knife, the cylinder of parenchyma tissue was rinsed with 70 % ethanol and flame-sterilized. Apoplastic sap from the internodes was collected by centrifugation according to Dong et al. (1994), and serial dilutions were streaked on tryptone soybean agar (TSA) and incubated at 30 u C for 7-10 days. After flaming, no contamination was observed when several stem pieces were incubated on TSA plates.Strain GR21 T was grown on nutrient agar for 48 h at 22 u C to check for motility by phase-contrast microscopy using the hanging drop method. Gram staining was carried o...

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“…The predominant respiratory quinone is MK-7, major fatty acids are iso-C 16 : 0 , anteiso-C 15 : 0 and C 16 : 0 and predominant polar lipids are diphosphatidylglycerol, phsphatidylglycerol and phosphatidylethanolamine (Kämpfer et al, 2006). At the time of writing, it comprises 22 species with validly published names (http://www.bacterio.net/cohnella.html), including C. hongkongensis and C. thermotolerans (Kämpfer et al, 2006), C. laeviribosi (Cho et al, 2007), C. phaseoli (García-Fraile et al, 2008), C. damuensis (Luo et al, 2010), C. fontinalis (Shiratori et al, 2010), C. ginsengisoli and C. yongneupensis (Kim et al, 2010), C. luojiensis (Cai et al, 2010), C. terrae and C. xylanilytica (Khianngam et al, 2010a), C. thailandensis (Khianngam et al, 2010b), C. panacarvi (Yoon et al, 2007), C. cellulosilytica (Khianngam et al, 2012), C. soli and C. suwonensis (Kim et al, 2011), C. boryungensis (Yoon & Jung, 2012), C. arctica (Jiang et al, 2012), C. ferri (Mayilraj et al, 2013), C. formosensis (Hameed et al, 2013), C. lupini (Flores-Félix et al, 2014) and C. rhizosphaerae (Kämpfer et al, 2014) and the species 'Cohnella plantaginis' has been proposed but its name is not validly published. The aim of this study was to elucidate the taxonomic position of strain D45 T based on phenotypic, chemotaxonomic characterization and 16S rRNA gene sequence analysis.…”

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Cohnella nanjingensis sp. nov., an extracellular polysaccharide-producing bacterium isolated from soil

Huang

Bao

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, rod-shaped, endospore-forming bacterium, strain D45 T , was isolated from soil in Nanjing, China. The organism grew optimally at 30 6C, pH 7.0 and with 0 % NaCl (w/v). The 16S rRNA gene sequence of the isolate showed similarities lower than 97 % with respect to species of the genus Cohnella. The predominant respiratory quinone was MK-7, with MK-6 present as a minor component; anteiso-C 15 : 0 and iso-C 16 : 0 were the major fatty acids. The polar lipids of strain D45 T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, four phospholipids, two glycolipids, one aminolipid and two lipids. The DNA G+C content was 59.5 mol%. DNA-DNA hybridization of the isolate with two reference strains showed relatedness values of 33.4 % with Cohnella ginsengisoli DSM 18997 T and 25.8 % with Cohnella thermotolerans DSM 17683 T . The phylogenetic, chemotaxonomic and phenotypic data supported the classification of strain D45 T as a representative of a novel species of the genus Cohnella, for which the name Cohnella nanjingensis sp. nov. is proposed. The type strain is D45 T (5CCTCC AB 2014067 T 5DSM 28246 T ).

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Cohnella laeviribosi sp. nov., isolated from a volcanic pond

Cited by 41 publications

References 30 publications

Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils

Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils

Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane

Cohnella nanjingensis sp. nov., an extracellular polysaccharide-producing bacterium isolated from soil

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