COI barcodes for identification of <i>Merodon</i> hoverflies (Diptera, Syrphidae) of Lesvos Island, Greece

Ståhls, Gunilla; Vujić, Ante; Pérez‐Bañón, Celeste; Radenković, Snežana; Petanidou, Theodora

doi:10.1111/j.1755-0998.2009.02592.x

Cited by 74 publications

(106 citation statements)

References 15 publications

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“…nov.), previously revealed by Ståhls et al (2009 Popović et al 2014Popović et al , 2015 can be explained by recent speciation and by the founder effect associated with postglacial recolonization of northern Europe (Shikano et al 2010). Both species share an identical haplotype of the COI 3'-end (Popović et al 2014), although the 5'-end fragment haplotypes of DNA barcodes are not shared, they give low support in cluster analyses (Popović et al 2015).…”

Section: Discussionmentioning

confidence: 95%

“…nova 2 in Ståhls et al (2009) and Ricarte et al (2012). Ståhls et al (2009) assumed it to be a distinct species based on the different COI barcode sequences obtained from two specimens morphologically similar to M. avidus taken from Lesvos Island. In the key prepared as supporting material for Ståhls et al (2009), M. avidus and M. sp.…”

Section: Remarksmentioning

confidence: 99%

“…Ståhls et al (2009) assumed it to be a distinct species based on the different COI barcode sequences obtained from two specimens morphologically similar to M. avidus taken from Lesvos Island. In the key prepared as supporting material for Ståhls et al (2009), M. avidus and M. sp. nova 2 key out together, without any morphological differences being described.…”

Section: Remarksmentioning

confidence: 99%

“…Furthermore, it is important to note that species of the M. avidus complex are not distinguishable by traditional visual identification of the structures of male genitalia under a stereo microscope. Additionally, in their analysis of COI barcodes of all Merodon species from Lesvos Island (Greece), Ståhls et al (2009) revealed the presence of one cryptic taxon (Merodon sp. nova 2, herein described as M. megavidus sp.…”

Section: Introductionmentioning

confidence: 99%

“…Taxa of the Merodon avidus complex have been the subject of many studies in the last decade due to perceived taxonomic difficulties (Milankov et al 2001Ståhls et al 2009). The M. avidus complex is characterized by a considerable morphological variability, especially in the coloration of the antennae, thorax, abdomen and legs (Popović et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Defining species boundaries in the Merodon avidus complex (Diptera, Syrphidae) using integrative taxonomy, with the description of a new species

Ačanski

Vujić

Djan

et al. 2016

EJT

543

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Abstract. Several recent studies have detected and described complexes of cryptic and sibling species in the genus Merodon (Diptera, Syrphidae). One representative of these complexes is the Merodon avidus complex that contains four sibling species, which have proven difficult to distinguish using traditional morphological characters. In the present study, we use two geometric morphometric approaches, as well as molecular characters of the 5'-end of the mtDNA COI gene, to delimit sibling taxa. Analyses based on these data were used to strengthen species boundaries within the complex, and to validate the status of a previously-recognized cryptic taxon from Lesvos Island (Greece), here described as Merodon megavidus Vujić & Radenković sp. nov. Geometric morphometric results of both wing and surstylus shape confirm the present classification for three sibling species-M. avidus (Rossi, 1790), M. moenium Wiedemann in Meigen, 1822 and M. ibericus Vujić, 2015-and, importantly, clearly discriminate the newly-described taxon Merodon megavidus sp. nov. In addition to our geometric morphometric results, supporting characters were obtained from molecular analyses of mtDNA COI sequences, which clearly differentiated M. megavidus sp. nov. from the other members of the M. avidus complex. Molecular analyses revealed that the earliest divergence of M. ibericus occurred around 800 ky BP, while the most recent separation happened between M. avidus and M. moenium around 87 ky BP.

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Section: Discussionmentioning

confidence: 95%

Section: Remarksmentioning

confidence: 99%

Section: Remarksmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Defining species boundaries in the Merodon avidus complex (Diptera, Syrphidae) using integrative taxonomy, with the description of a new species

Ačanski

Vujić

Djan

et al. 2016

EJT

543

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Phylogeographic patterns of Merodon hoverflies in the Eastern Mediterranean region: revealing connections and barriers

Ståhls

Vujić

Petanidou

et al. 2016

Ecology and Evolution

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We investigated the phylogeographic patterns of Merodon species (Diptera, Syrphidae) in the Eastern Mediterranean. Ten species were sampled on five different islands and mainland sites as a minimum. All samples were screened for their mtDNA COI barcode haplotype diversity, and for some samples, we additionally generated genomic fingerprints. The recently established zoogeographic distribution categories classify these species as having (1) Balkan distribution; (2) Anatolian distribution; (3) continental areas and large islands distribution; and (4) with wide distribution. The ancestral haplotypes and their geographical localities were estimated with statistical parsimony (TCS). TCS networks identified as the ancestral haplotype samples that originated from localities situated within the distributional category of the species in question. Strong geographical haplotype structuring was detected for many Merodon species. We were particularly interested to test the relative importance of current (Aegean Sea) and past Mid‐Aegean Trench) barriers to dispersal for Merodon flies in the Aegean. We employed phylogenetic β‐diversity (Pβ total) and its partition in replacement (Pβ repl) and richness difference (Pβ rich) to test the importance of each explanatory variable (interisland distance, MAT, and island area) in interisland differences using partial Mantel tests and hierarchical partitioning of variation. β‐Analyses confirmed the importance of both current and past barriers to dispersal on the evolution of group. Current interisland distance was particularly important to explain the replacement of haplotypes, while the MAT was driving differences in richness of haplotypes, revealing the MAT as a strong past barrier whose effects are still visible today in the phylogenetic history of the clade in the Aegean. These results support the hypothesis of a highly restricted dispersal and gene flow among Merodon populations between islands since late Pleistocene. Additionally, patterns of phylogeographic structure deduced from haplotype connections and ISSR genome fingerprinting data revealed a few putative cases of human‐mediated transfers of Merodon spp.

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DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control

Gojković

Francuski

Ludoški

et al. 2020

Ecology and Evolution

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With the advent of integrated pest management, the conservation of indigenous populations of natural enemies of pest species has become a relevant practice, necessitating the accurate identification of beneficial species and the inspection of evolutionary mechanisms affecting the long‐time persistence of their populations. The long hoverfly, Sphaerophoria scripta, represents one of the most potent aphidophagous control agents due to a worldwide distribution and a favorable constellation of biological traits. Therefore, we assessed five European S. scripta populations by combining molecular (cytochrome c oxidase subunit I‐ COI, internal transcribed spacer 2‐ ITS2, and allozyme loci) and morphological (wing size and shape) characters. COI sequences retrieved in this study were conjointly analyzed with BOLD/GenBank sequences of the other Sphaerophoria species to evaluate whether COI possessed a sufficient diagnostic value as a DNA barcode marker to consistently delimit allospecific individuals. Additionally, the aforementioned characters were used to inspect the population structure of S. scripta in Europe using methods based on individual‐ and population‐based genetic differences, as well as geometric morphometrics of wing traits. The results indicate numerous shared COI haplotypes among different Sphaerophoria species, thus disqualifying this marker from being an adequate barcoding region in this genus. Conversely, the analyses of population structuring revealed high population connectivity across Europe, therefore indicating strong tolerance of S. scripta to environmental heterogeneity. The results imply a multilocus approach as the next step in molecular identification of different Sphaerophoria species, while confirming the status of S. scripta as a powerful biocontrol agent of economically relevant aphid pests.

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COI barcodes for identification of Merodon hoverflies (Diptera, Syrphidae) of Lesvos Island, Greece

Cited by 74 publications

References 15 publications

Defining species boundaries in the Merodon avidus complex (Diptera, Syrphidae) using integrative taxonomy, with the description of a new species

Defining species boundaries in the Merodon avidus complex (Diptera, Syrphidae) using integrative taxonomy, with the description of a new species

Phylogeographic patterns of Merodon hoverflies in the Eastern Mediterranean region: revealing connections and barriers

DNA barcode assessment and population structure of aphidophagous hoverfly Sphaerophoria scripta: Implications for conservation biological control

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