2019
DOI: 10.1021/jacs.8b13604
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Coiled-Coil-Mediated Assembly of an Icosahedral Protein Cage with Extremely High Thermal and Chemical Stability

Abstract: The organization of protein molecules into higher-order nanoscale architectures is ubiquitous in Nature and represents an important goal in synthetic biology. Here we describe the symmetrydirected design of a hollow protein cage with dimensions similar to those of many icosahedral viruses. The cage was constructed based on icosahedral symmetry by genetically fusing a trimeric protein (TriEst) to a small pentameric de novo-designed coiled coil domain, separated by a flexible oligo-glycine linker sequence. Scree… Show more

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Cited by 58 publications
(61 citation statements)
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“…38,39 Along the same lines, coiled-coil forming peptides afford ideal glues for connecting protein building blocks with designed symmetries. 40,41 For example, a trimeric esterase equipped with three-, four-or ve-helix coiled coils forms tetrahedral, 42 octahedral, 43 or icosahedral 44 cages, respectively ( Fig. 3II).…”
Section: Cage Formation Through Protein-protein Interactionsmentioning
confidence: 99%
“…38,39 Along the same lines, coiled-coil forming peptides afford ideal glues for connecting protein building blocks with designed symmetries. 40,41 For example, a trimeric esterase equipped with three-, four-or ve-helix coiled coils forms tetrahedral, 42 octahedral, 43 or icosahedral 44 cages, respectively ( Fig. 3II).…”
Section: Cage Formation Through Protein-protein Interactionsmentioning
confidence: 99%
“…[26][27][28][29][30] In a different concept Marsh and colleagues combine de novo coiled-coil units and natural oligomeric proteins to render defined protein nanocages. [41][42][43][44] Here we present a self-assembling hairpin designs that form two distinct suporamolecular assemblies, namely, closed nanoparticles and extended sheets. These designs combine ideas from the SAGEs and Burkhard's nanoparticles.…”
Section: Introductionmentioning
confidence: 99%
“…Apart from the electrostatic interaction and metal coordination mentioned in the previous two sections, other interactions such as hydrophobic interaction, 49,[69][70][71] coilcoil interaction [72][73][74][75][76] and specific icosahedral symmetry protein-protein interactions 77,78 can also be used to induce assembly of VLPs at neutral pH.…”
Section: Other Supramolecular Interactions Induced Assembly Of Viruslmentioning
confidence: 99%
“…Another approach to induce assembly of higher ordered nanoscale cages is to incorporate de novo-designed coiled-coil domain into the subunit of protein cages (Figure 2.6 B) which results in symmetrical icosahedral VLPs. 72 With the development of the understanding of assembly pathway and mechanism, computational design of proteins based on symmetry principles, have been widely used in the last decades to create ordered assembly of icosahedral protein cages 79-81 ( Figure 2.6 C).…”
Section: Conjugation Of Function Fragments To Capsid Proteins Enablesmentioning
confidence: 99%
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