Coilin is rapidly recruited to UVA-induced DNA lesions and γ-radiation affects localized movement of Cajal bodies

Bártová, Eva; Foltánková, Veronika; Legartová, Soňa; Sehnalová, Petra; Sorokin, Dmitry V.; Suchánková, Jana; Kozubek, Stanislav

doi:10.4161/nucl.29229

Cited by 23 publications

(26 citation statements)

References 35 publications

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“…BRCA1 appeared at DNA lesions between 20 and 80 min after local micro‐irradiation (Figure A, an interval of observation up to 50 min was normalised by shown for all proteins). Intriguingly, the most rapid accumulation at DNA lesions was observed for GFP‐tagged coilin (Bártová et al., ), BMI1 (see Šustáčková et al., ); here we showed accumulation of endogenous BMI1 and RFP‐tagged PCNA (Figure A).…”

Section: Resultssupporting

confidence: 69%

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Distinct kinetics of DNA repair protein accumulation at DNA lesions and cell cycle‐dependent formation of γH2AX‐ and NBS1‐positive repair foci

Suchánková

Kozubek

Legartová

et al. 2015

Biology of the Cell

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Section: Resultssupporting

confidence: 69%

“…() and Bártová et al. (). Here, we compared recruitment of BMI1 and coilin to DNA lesions with the well‐known DNA damage‐related protein, BRCA1, which appears rapidly at DNA damage sites (Scully et al., ).…”

Section: Discussionmentioning

confidence: 98%

Distinct kinetics of DNA repair protein accumulation at DNA lesions and cell cycle‐dependent formation of γH2AX‐ and NBS1‐positive repair foci

Suchánková

Kozubek

Legartová

et al. 2015

Biology of the Cell

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“…Previous studies made similar conclusions related to the movement of the nuclear domain, for example, different classes of PML bodies or Cajal bodies (CBs) which are highly mobile in non-irradiated cells. 27,29,33 Moreover, we recently demonstrated that local CB motion decreases after g-irradiation, which is in agreement with data that radiation induces changes in nuclear domain trajectories (compare 32 with Fig. 2Bc, white arrows).…”

Section: Discussionsupporting

confidence: 78%

“…2A-D, 4A, 4B, or Ref. 14,32 ). In this study, we analyzed the GFP-UBF1-positive regions of nucleoli after exposure to UVA or g-radiation.…”

Section: Discussionmentioning

confidence: 99%

Localized movement and morphology of UBF1-positive nucleolar regions are changed by γ-irradiation in G2 phase of the cell cycle

Sorokin

Stixová

Sehnalová

et al. 2015

Nucleus

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(2015) Localized movement and morphology of UBF1-positive nucleolar regions are changed by γ-irradiation in G2 phase of the cell cycle, Nucleus, 6:4, 301-313, DOI: 10.1080/19491034.2015 To link to this article: https://doi.org/10. 1080/19491034.2015 Abbreviations: 53BP1, p53-binding protein; ACT-D, actinomycin D; ATM, ataxia telangiectasia mutated; ATR, ATM and Rad3-related protein; AR, area ratio (area of nucleolus/area of nucleus); BrdU, 5-bromo-2 0 -deoxy-uridine; BER, base excision repair; CBs, Cajal bodies; DDR, DNA damage response; CPDs, cyclobutane pyrimidine dimers; DMEM, Dulbecco's modified Eagle's medium; DNA-PK, DNA-dependent protein kinase; DSBs, double-strand breaks; FA, foci area; NF, number of foci; FI, fluorescence intensity; FRAP, fluorescence recovery after photobleaching; GGR, global genome repair; GFP, green fluorescence protein; HP1, heterochromatin protein 1; HR, homologous recombination; HRR, homologous recombination repair; iMEFs, immortalized mouse embryonic fibroblasts; LR, local radius; NBs, nuclear bodies; NER, nucleotide excision repair; NHEJ, non-homologous end-joining; nA, nucleolus area; NA, nucleus area; PBS, phosphate-buffered saline; P2A, compactness; PCR, polymerase chain reaction; PML, promyelocytic leukemia bodies; rDNA, ribosomal DNA; RNA Pol II, RNA polymerase II; ROI, region of interest; RPA, replicationrelated protein A; SEM, standard error of the mean; SDS, sodium dodecyl sulfate; TCR, transcription-coupled NER; UBF1, upstream binding factor 1; UV, ultraviolet.The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or g-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of g-rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that g-radiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive foci. Radiation-induced nucleoli rearrangement was observed particularly in G2 phase of the cell cycle, indicating repair of ribosomal genes in G2 phase and implying that nucleoli are less stable, thus sensitive to radiation, in G2 phase.

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“…18 The interaction between coilin and WRAP53 could also explain why coilin is found at sites of DNA damage, and CBs are sensitive to UV irradiation. 55,56 While coilin function during DNA repair is unclear, WRAP53 was recently shown to be critical for DNA double-strand break repair. 57 Independent work found coilin associated with DNA repair factor Ku.…”

Section: Coilin Historymentioning

confidence: 99%

Coilin: The first 25 years

2015

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Coilin is rapidly recruited to UVA-induced DNA lesions and γ-radiation affects localized movement of Cajal bodies

Abstract: (2014) Coilin is rapidly recruited to UVA-induced DNA lesions and γ-radiation affects localized movement of Cajal bodies, Nucleus, 5:3,[269][270][271][272][273][274][275][276][277]

Cited by 23 publications

References 35 publications

Distinct kinetics of DNA repair protein accumulation at DNA lesions and cell cycle‐dependent formation of γH2AX‐ and NBS1‐positive repair foci

Distinct kinetics of DNA repair protein accumulation at DNA lesions and cell cycle‐dependent formation of γH2AX‐ and NBS1‐positive repair foci

Localized movement and morphology of UBF1-positive nucleolar regions are changed by γ-irradiation in G2 phase of the cell cycle

Coilin: The first 25 years

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