Coincidence studies of quasimolecular electron emission in 700-keV<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msup><mml:mrow><mml:mi mathvariant="normal">Ar</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:msup></mml:mrow></mml:math>-Kr collisions

Salicortin, a natural product abundant in most members of the Salicaceae family, is a mechanism-based inactivator of Agrobacterium faecalis beta-glucosidase. Inactivation is delayed in the presence of competitive inhibitors, thereby demonstrating the requirement for an enzyme-bound salicortin before inactivation. Product studies suggest that inactivation proceeds via a quinone methide intermediate formed by the fragmentation of the aglycone of salicortin while it is bound to the enzyme. Tryptic digest and HPLC/MS studies confirm the role of quinone methide attack and also show that the enzyme undergoes multiple modifications. In addition, when the inactivation was run in the presence of a mutant inactive form of the enzyme, HPLC/MS analyses clearly showed no modification of the mutant enzyme, demonstrating that the quinone methide does not exist in free solution and suggesting that inactivation is active-site directed.

show abstract

Salicortin: a repeat-attack new-mechanism-based Agrobacterium faecalis β-glucosidase inhibitor

Zhu

Withers

Reichardt

et al. 1998

Biochemical Journal

View full text Add to dashboard Cite

show abstract

ArLAuger-electron emission in 0.4-, 0.8-, and 1.6-MeVAr+-Kr andKr+<

1990

Phys. Rev. A

View full text Add to dashboard Cite

Continuum Electron Spectra Arising from a Dynamical Variation of the Molecular-Orbitals in Slow Kr+Xe Collisions

Shanker

Faust

1996

J. Phys. Soc. Jpn.

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Coincidence studies of quasimolecular electron emission in 700-keVAr2+-Kr collisions

Cited by 3 publications

References 13 publications

Salicortin: a repeat-attack new-mechanism-based Agrobacterium faecalis β-glucosidase inhibitor

Salicortin: a repeat-attack new-mechanism-based Agrobacterium faecalis β-glucosidase inhibitor

ArLAuger-electron emission in 0.4-, 0.8-, and 1.6-MeVAr+-Kr andKr+<

Continuum Electron Spectra Arising from a Dynamical Variation of the Molecular-Orbitals in Slow Kr+Xe Collisions

Contact Info

Product

Resources

About