Coke-derived graphene quantum dots as fluorescence nanoquencher in DNA detection

Yew, Ying Teng; Loo, Adeline Huiling; Sofer, Zdeněk; Klímová, Kateřina; Pumera, Martin

doi:10.1016/j.apmt.2017.01.002

Cited by 58 publications

(34 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both GQDs-1 and GQDs-2 are rich of hydrophilic groups such as carboxyl and hydroxyl groups that are formed in the chemical oxidation step of CTP in hydrogen peroxide. The abundant hydrophilic groups greatly improve the aqueous solubility of the CQDs and support their applications in aqueous systems [ 37 ]. Compared to graphene or graphene-based nanomaterials, GQDs have better water solubility, and can combine with a variety of compounds by the intermolecular π–π interaction [ 15 ].…”

Section: Resultsmentioning

confidence: 99%

Green Preparation of High Yield Fluorescent Graphene Quantum Dots from Coal-Tar-Pitch by Mild Oxidation

Liu

Zhang

et al. 2018

Nanomaterials

View full text Add to dashboard Cite

Coal tar pitch (CTP), a by-product of coking industry, has a unique molecule structure comprising an aromatic nucleus and several side chains bonding on this graphene-like nucleus, which is very similar to the structure of graphene quantum dots (GQDs). Based on this perception, we develop a facile approach to convert CTP to GQDs only by oxidation with hydrogen peroxide under mild conditions. One to three graphene layers, monodisperse GQDs with a narrow size distribution of 1.7 ± 0.4 nm, are obtained at high yield (more than 80 wt. %) from CTP. The as-produced GQDs are highly soluble and strongly fluorescent in aqueous solution. This simple strategy provides a feasible route towards the commercial synthesis of GQDs for its cheap material source, green reagent, mild condition, and high yield.

show abstract

Section: Resultsmentioning

confidence: 99%

Green Preparation of High Yield Fluorescent Graphene Quantum Dots from Coal-Tar-Pitch by Mild Oxidation

Liu

Zhang

et al. 2018

Nanomaterials

View full text Add to dashboard Cite

show abstract

“…in 2010, GQDs have found plenty of applications in optoelectronics,, photodetections,, photovoltics, light‐emitting diodes,, and plasmonics . Due to their ability to function as Fluorescence Resonance Energy Transfer (FRET) acceptors, GQDs have also shown significant potential as nano‐quenchers for bioassays, in which organic fluorescent dyes (fluorophores) are used as labels for the detection of nucleic acids, proteins, and other low molecular weight compounds …”

Section: Introductionmentioning

confidence: 99%

Unravelling the Aptamer‐Analyte Interaction Dynamics through Fluorescence Quenching in Graphene Quantum Dots (GQDs) Based Homogeneous Assays

Ang

Bonanni

2019

ChemPlusChem

View full text Add to dashboard Cite

Graphene quantum dots (GQDs) are used here as a biosensing platform for the recognition of the major food contaminant ochratoxin A (OTA), with a fluorescently labelled DNA aptamer (FAM OTA aptamer) functioning as the biorecognition element. The detection principle lies in the formation of noncovalent interactions between the FAM OTA aptamer and the GQD surface, and the consequent fluorescence quenching. The further change in the fluorescence signal, induced by the formation of the FAM OTA Aptamer/OTA conjugate during the detection step, could then be correlated to the presence and concentration of the target analyte. Upon tuning the concentration of GQDs, a switch in the biorecognition mechanism occurred. Specifically, while a lower GQD concentration (0.060 mg/mL) resulted in a restoration of the fluorescence intensity upon incubation with OTA, a higher GQD concentration (0.150 mg/mL) provided a further quenching of the final fluorescence intensity. Upon further calibration study, it was discovered that the latter mechanism provided a better option in terms of linearity of response, detection limit and selectivity. exchange column (Millipore) with a resistivity of 18.2 MΩ cm. A 0.025 M Tris-HCl buffer solution (pH 7.9, 0.3 M NaCl, 20 mM CaCl 2 ) was employed in this study. 2 3 4 5 6 7 8

show abstract

“…In addition, graphene-based materials are known as energy acceptors in energy transfer reactions due to their peculiar electronic properties [ 17 ]. As photophysical calculations have revealed energy transfer phenomena, GO can be used as a quencher (acceptor) of electronic excited states in fluorescent dyes (donors) resulting in the phenomenon of fluorescence resonance energy transfer (FRET) [ 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Robust Magnetized Graphene Oxide Platform for In Situ Peptide Synthesis and FRET-Based Protease Detection

Kim

Lee

Yoon

et al. 2020

Sensors

View full text Add to dashboard Cite

Graphene oxide (GO)/peptide complexes as a promising disease biomarker analysis platform have been used to detect proteolytic activity by observing the turn-on signal of the quenched fluorescence upon the release of peptide fragments. However, the purification steps are often cumbersome during surface modification of nano-/micro-sized GO. In addition, it is still challenging to incorporate the specific peptides into GO with proper orientation using conventional immobilization methods based on pre-synthesized peptides. Here, we demonstrate a robust magnetic GO (MGO) fluorescence resonance energy transfer (FRET) platform based on in situ sequence-specific peptide synthesis of MGO. The magnetization of GO was achieved by co-precipitation of an iron precursor solution. Magnetic purification/isolation enabled efficient incorporation of amino-polyethylene glycol spacers and subsequent solid-phase peptide synthesis of MGO to ensure the oriented immobilization of the peptide, which was evaluated by mass spectrometry after photocleavage. The FRET peptide MGO responded to proteases such as trypsin, thrombin, and β-secretase in a concentration-dependent manner. Particularly, β-secretase, as an important Alzheimer’s disease marker, was assayed down to 0.125 ng/mL. Overall, the MGO platform is applicable to the detection of other proteases by using various peptide substrates, with a potential to be used in an automated synthesis system operating in a high throughput configuration.

show abstract

Coke-derived graphene quantum dots as fluorescence nanoquencher in DNA detection

Cited by 58 publications

References 77 publications

Green Preparation of High Yield Fluorescent Graphene Quantum Dots from Coal-Tar-Pitch by Mild Oxidation

Green Preparation of High Yield Fluorescent Graphene Quantum Dots from Coal-Tar-Pitch by Mild Oxidation

Unravelling the Aptamer‐Analyte Interaction Dynamics through Fluorescence Quenching in Graphene Quantum Dots (GQDs) Based Homogeneous Assays

Robust Magnetized Graphene Oxide Platform for In Situ Peptide Synthesis and FRET-Based Protease Detection

Contact Info

Product

Resources

About