Colchicine-induced tetraploidy influences morphological and cytological characteristics and enhances accumulation of anthocyanins in a red-fleshed radish (Raphanus sativus L.)

Chen, Fabo; Gao, Jian; Li, Wenbo; Liu, Yi; Fang, Ping; Peng, Zhihang

doi:10.1007/s13580-021-00363-w

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…Previous studies have reported that mitotic inhibitors, such as oryzalin, induce chromosome doubling in ornamental plants, which in turn results in polyploidization (Castro et al, 2003;Pliankong et al, 2017). Polyploidization has provided beneficial effects for many valuable plants including fruit, vegetable, and ornamental crops (Chen et al, 2021;Cho et al, 2021;Kim et al, 2022). Mutants exhibiting fewer, shorter, and thicker leaves in Echeveria cultivars reflect compactness or compact growth-a desired characteristic in ornamental potted plant production (van Huylenbroeck, 2018).…”

Section: Survival and Mutation Ratementioning

confidence: 99%

Phenotypic Evaluation and Nuclear DNA Content Analysis of Oryzalin-Induced Echeveria Mutant Cultivars

Cabahug-Braza,

Tran,

Lim

et al. 2023

HST

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Succulent plants are in high demand in the floriculture industry owing to their peculiar leaf arrangement, water-efficient characteristics, and wide color variation. In particular, the succulent genus Echeveria is preferred by many plant collectors and hobbyists. Therefore, continuous breeding programs for these succulents are underway to produce novel cultivars with new features, leading to higher market prices. As opposed to conventional breeding, chemical mutagenesis can achieve this goal. The use of oryzalin, an anti-microtubule drug, is effective to induce plant mutations and less hazardous to human health. Thus, in earlier work, we explored the use of oryzalin to induce mutant Echeveria cultivars, specifically the 'Brave', 'Momotarou', and 'Sistar'. Briefly, different concentrations (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) and dipping durations (3, 6, 9, and 12 h) of oryzalin were employed for chemical mutagenesis. Oryzalin produced Echeveria mutants with fewer, larger, darker, and thicker leaves compared to control plants. A stomatal analysis revealed that the mutants exhibited larger but fewer stomata and a lower epidermal cell density, consistent with polyploidization. Future studies involving chromosome counting and flow cytometry of putative oryzalin-induced mutants and their next generations are warranted to identify whether these alternations are reversible. Our findings will be useful for the mutation breeding of closely related ornamentals or other potted plants and development of new cultivars with novel traits.

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Section: Survival and Mutation Ratementioning

confidence: 99%

Phenotypic Evaluation and Nuclear DNA Content Analysis of Oryzalin-Induced Echeveria Mutant Cultivars

Cabahug-Braza,

Tran,

Lim

et al. 2023

HST

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In vitro polyploidy induction in ginger (Zingiber officinale Rosc.) cv. Himgiri: morpho-physiological, biochemical, and molecular evaluation of regenerants

Kumar,

Thakur,

Thakur

et al. 2024

In Vitro Cell.Dev.Biol.-Plant

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Polyploids of Brassicaceae: Genomic Insights and Assembly Strategies

Jeon,

Kim

2024

Plants

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The Brassicaceae family is distinguished by its inclusion of high-value crops such as cabbage, broccoli, mustard, and wasabi, all noted for their glucosinolates. In this family, many polyploidy species are distributed and shaped by numerous whole-genome duplications, independent genome doublings, and hybridization events. The evolutionary trajectory of the family is marked by enhanced diversification and lineage splitting after paleo- and meso-polyploidization, with discernible remnants of whole-genome duplications within their genomes. The recent neopolyploidization events notably increased the proportion of polyploid species within the family. Although sequencing efforts for the Brassicaceae genome have been robust, accurately distinguishing sub-genomes remains a significant challenge, frequently complicating the assembly process. Assembly strategies include comparative analyses with ancestral species and examining k-mers, long terminal repeat retrotransposons, and pollen sequencing. This review comprehensively explores the unique genomic characteristics of the Brassicaceae family, with a particular emphasis on polyploidization events and the latest strategies for sequencing and assembly. This review will significantly improve our understanding of polyploidy in the Brassicaceae family and assist in future genome assembly methods.

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Colchicine-induced tetraploidy influences morphological and cytological characteristics and enhances accumulation of anthocyanins in a red-fleshed radish (Raphanus sativus L.)

Cited by 3 publications

References 32 publications

Phenotypic Evaluation and Nuclear DNA Content Analysis of Oryzalin-Induced Echeveria Mutant Cultivars

Phenotypic Evaluation and Nuclear DNA Content Analysis of Oryzalin-Induced Echeveria Mutant Cultivars

In vitro polyploidy induction in ginger (Zingiber officinale Rosc.) cv. Himgiri: morpho-physiological, biochemical, and molecular evaluation of regenerants

Polyploids of Brassicaceae: Genomic Insights and Assembly Strategies

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