Colchicine inhibition of insulin induction of stearoyl-CoA desaturase and fatty acid synthetase in cultured avian liver explants

Kasturi, R; Joshi, V.C.; Wakil, Salih J.

doi:10.1016/0003-9861(84)90476-4

Cited by 15 publications

(11 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our studies, we used colchicine to determine that microtubules are critical to fibronectin mRNA translation. Others have reported that colchicine treatment inhibits synthesis of collagen, 34 stearoyl coenzyme A desaturase, and fatty acid synthetase 35 without affecting total protein synthesis, but the mechanism was unexplored. Colchicine has also been demonstrated to inhibit general translation of mRNA in hepatectomized rats without affecting cell proliferation by inducing a global dissociation of membrane-bound polysomes.…”

Section: Discussionmentioning

confidence: 99%

Microtubule Involvement in Translational Regulation of Fibronectin Expression by Light Chain 3 of Microtubule-Associated Protein 1 in Vascular Smooth Muscle Cells

Zhou

Rabinovitch

1998

Circulation Research

View full text Add to dashboard Cite

Abstract-Our previous studies suggested that enhanced fibronectin mRNA translation in ductus arteriosus compared with aortic smooth muscle cells is related to increased expression of light chain 3 (LC3) of microtubule-associated protein 1, which binds an AU-rich element in the 3Ј untranslated region of fibronectin mRNA. We therefore hypothesized that microtubules are involved in LC3-mediated fibronectin mRNA translational regulation. In this study we show that disruption of microtubules by colchicine inhibits fibronectin mRNA translation in cultured ductus arteriosus smooth muscle cells. We proposed that the mechanism might be related to decreased docking of fibronectin mRNA on the translational machinery, ie, membrane-bound polysomes on rough endoplasmic reticulum, and confirmed this by Northern blot analysis. To investigate the mechanism further, we carried out polysome analysis using sucrose gradient centrifugation and fractionation and studied the polysomal distribution of fibronectin mRNA and LC3 protein in the sucrose gradient by using RNase protection assay and Western immunoblotting, respectively. Colchicine treatment shifts fibronectin mRNA from the fractions containing membrane-bound polysomes to the fractions carrying free polysomes and concomitantly decreases the amount of LC3 protein in the fractions containing membrane-bound polysomes. Furthermore, an EDTA-release experiment demonstrates that LC3 protein associates with the 60S ribosomal subunit. Our data support the concept that microtubules may function with LC3 to facilitate sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. (Circ Res. 1998;83:481-489.)

show abstract

Section: Discussionmentioning

confidence: 99%

Microtubule Involvement in Translational Regulation of Fibronectin Expression by Light Chain 3 of Microtubule-Associated Protein 1 in Vascular Smooth Muscle Cells

Zhou

Rabinovitch

1998

Circulation Research

View full text Add to dashboard Cite

show abstract

“…Reduced hepatic phospholipids after feeding low quality protein or protein-deficient diets have been reported by several investigators 2 -4 The active role of phosphatidylcholine (PC) in microsomal drug metabolism has been well established 5; PC probably helps in electron transfer processes and also in accumulating nonpolar substrates. Conney et al 6 have reported that malathion, chlorothion and parathion inhibit the in vitro microsomal metabolism of testosterone. In the liver, PC is synthesized by more than one pathway; however, the major pathway is the CDP-choline pathway 7.…”

mentioning

confidence: 98%

A signoidal relationship between liver stearoyl CoA desaturase activity and serum hormone concentrations caused by streptozocin and its antagonists

et al. 1988

View full text Add to dashboard Cite

Stearoyl CoA desaturase activity in liver microsomes, and insulin, thyroxine, and triiodothyronine levels in serum were measured after administration of streptozocin (STZ) and its antagonists to rats. The effect of STZ, which caused hyperglycemia and inhibited the desaturase activity, was antagonized by 2-desoxyglucose and 3-O-methyl-glucose; 1-O-methyl-3-desoxyglucose and 1-O-methyl-3-O-methylglucose were without any effect. The enzyme activity plotted against insulin levels showed a broad sigmoidal curve, whereas the activities versus thyroid hormone levels showed steeper sigmoidal curves.

show abstract

“…Insulin binding to cell surface receptors was not inhibited by colchicine in chicken liver cell cultures [9]. When colchicine was added after insulin incubation, no induction of lipogenic enzyme activity was observed.…”

Section: Introductionmentioning

confidence: 86%

“…When colchicine was added after insulin incubation, no induction of lipogenic enzyme activity was observed. Kasturi and colleagues [9] suggested an intermediate colchicine-sensitive component, which may be involved in the action of insulin, subsequent to the hormonal binding to cell receptors. Whether colchicine interferes with the endocytosis of insulin receptors is controversial.…”

Section: Introductionmentioning

confidence: 98%

Chronic colchicine treatment does not impair glucose tolerance in familial Mediterranean fever patients

Burstein

Seidman

Zemer

et al. 1997

European Journal of Clinical Pharmacology

View full text Add to dashboard Cite

show abstract

Colchicine inhibition of insulin induction of stearoyl-CoA desaturase and fatty acid synthetase in cultured avian liver explants

Cited by 15 publications

References 32 publications

Microtubule Involvement in Translational Regulation of Fibronectin Expression by Light Chain 3 of Microtubule-Associated Protein 1 in Vascular Smooth Muscle Cells

Microtubule Involvement in Translational Regulation of Fibronectin Expression by Light Chain 3 of Microtubule-Associated Protein 1 in Vascular Smooth Muscle Cells

A signoidal relationship between liver stearoyl CoA desaturase activity and serum hormone concentrations caused by streptozocin and its antagonists

Chronic colchicine treatment does not impair glucose tolerance in familial Mediterranean fever patients

Contact Info

Product

Resources

About