Cold acclimation is accompanied by complex responses of glycosylphosphatidylinositol (GPI)-anchored proteins in Arabidopsis

Takahashi, Daisuke; Kawamura, Yukio; Uemura, Matsuo

doi:10.1093/jxb/erw279

Cited by 52 publications

(48 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Roots of Pisum sativum grown under clinorotation also show shifts in membrane composition in sterol-enriched membrane domains known as "lipid rafts" (Kordyum et al, 2018). Membrane-bound GPI-APs, such as SKU5, localize to these lipid raft nanodomains and are important to stress acclimation, including spaceflightassociated stress (Minami et al, 2008;Mazars et al, 2014;Ferl et al, 2015;Takahashi et al, 2016Takahashi et al, , 2019Yeats et al, 2018). ABA signaling proteins also localize to these membrane domains in a sterol-dependent manner (Demir et al, 2013).…”

Section: Sku5 Mutation Produces a Dramatic Increase In Differential Gmentioning

confidence: 99%

“…SKU5 is a glycosylphosphatidylinositol-anchored protein (GPI-AP), and as such is a component of discrete plasma membrane nanodomains known as "lipid rafts" (Borner et al, 2005;Elortza et al, 2006;Chen et al, 2018;Mamode Cassim et al, 2019). These nanodomains of the plasma membrane and their associated GPI-APs play roles in stress response signaling at the interface between the cell wall and membrane, where they are envisioned to help shape the "signatures" that activate downstream plant adaptive responses to the stresses they experience (Minami et al, 2008;Takahashi et al, 2013Takahashi et al, , 2016Takahashi et al, , 2019Shabala et al, 2015;Yeats et al, 2018;Miki et al, 2019). The endocytosis of SKU5 and other GPI-APs is modulated across the root tip by salt and auxin treatments, linking the movement of these proteins to stress responses (Baral et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Root Skewing-Associated Genes Impact the Spaceflight Response of Arabidopsis thaliana

et al. 2020

View full text Add to dashboard Cite

The observation that plant roots skew in microgravity recently refuted the long-held conviction that skewing was a gravity-dependent phenomenon. Further, spaceflight root skewing suggests that specific root morphologies and cell wall remodeling systems may be important aspects of spaceflight physiological adaptation. However, connections between skewing, cell wall modification and spaceflight physiology are currently based on inferences rather than direct tests. Therefore, the Advanced Plant Experiments-03-2 (APEX-03-2) spaceflight study was designed to elucidate the contribution of two skewing-and cell wall-associated genes in Arabidopsis to root behavior and gene expression patterns in spaceflight, to assess whether interruptions of different skewing pathways affect the overall spaceflight-associated process. SPIRAL1 is a skewingrelated protein implicated in directional cell expansion, and functions by regulating cortical microtubule dynamics. SKU5 is skewing-related glycosylphosphatidylinositolanchored protein of the plasma membrane and cell wall implicated in stress response signaling. These two genes function in different cellular pathways that affect skewing on the Earth, and enable a test of the relevance of skewing pathways to spaceflight physiological adaptation. In this study, both sku5 and spr1 mutants showed different skewing behavior and markedly different patterns of gene expression in the spaceflight environment. The spr1 mutant showed fewer differentially expressed genes than its Col-0 wild-type, whereas sku5 showed considerably more than its WS wild-type. Developmental age played a substantial role in spaceflight acclimation in all genotypes, but particularly in sku5 plants, where spaceflight 4d seedlings had almost 10-times as many highly differentially expressed genes as the 8d seedlings. These differences demonstrated that the two skewing pathways represented by SKU5 and SPR1 have unique and opposite contributions to physiological adaptation to spaceflight. The spr1 response is less intense than wild type, suggesting that the loss of SPR1 positively impacts spaceflight adaptation. Conversely, the intensity of the sku5 responses suggests that the loss of SKU5 initiates a much more complex, deeper and more stress related response to spaceflight. This suggests that proper SKU5 function is important to spaceflight adaptation.

show abstract

Section: Sku5 Mutation Produces a Dramatic Increase In Differential Gmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Root Skewing-Associated Genes Impact the Spaceflight Response of Arabidopsis thaliana

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Additional differences could exist in the regulation of these genes during the diurnal cycle or in response to environmental factors. Some GPI-anchored proteins appear to be released to the apoplast (Takahashi et al, 2016), and this seems to be the case for a fraction of BGLC3 activity in 35S:BGLC3 lines (Fig. 4C).…”

Section: The Roles Of Xyloglucan B-glucosidasesmentioning

confidence: 83%

“…More recently, BGLC3 has been identified in a membrane fraction enriched in GPI-anchored proteins through phospholipase digestion (Takahashi et al, 2016). That study also analyzed an apoplastic fraction, where it identified BGLC1 but not BGLC3.…”

Section: Discussionmentioning

confidence: 94%

Soluble and Membrane-Bound β-Glucosidases Are Involved in Trimming the Xyloglucan Backbone

et al. 2016

View full text Add to dashboard Cite

ORCID IDs: 0000-0002-6534-5347 (J.S.); 0000-0001-6773-0583 (E.R.V.); 0000-0001-9053-4134 (N.I.); 0000-0002-2150-8538 (G.R.); 0000-0001-7203-0688 (I.Z.).In many flowering plants, xyloglucan is a major component of primary cell walls, where it plays an important role in growth regulation. Xyloglucan can be degraded by a suite of exoglycosidases that remove specific sugars. In this work, we show that the xyloglucan backbone, formed by (1→4)-linked b-D-glucopyranosyl residues, can be attacked by two different Arabidopsis (Arabidopsis thaliana) b-glucosidases from glycoside hydrolase family 3. While BGLC1 (At5g20950; for b-glucosidase active against xyloglucan 1) is responsible for all or most of the soluble activity, BGLC3 (At5g04885) is usually a membraneanchored protein. Mutations in these two genes, whether on their own or combined with mutations in other exoglycosidase genes, resulted in the accumulation of partially digested xyloglucan subunits, such as GXXG, GXLG, or GXFG. While a mutation in BGLC1 had significant effects on its own, lack of BGLC3 had only minor effects. On the other hand, double bglc1 bglc3 mutants revealed a synergistic interaction that supports a role for membrane-bound BGLC3 in xyloglucan metabolism. In addition, bglc1 bglc3 was complemented by overexpression of either BGLC1 or BGLC3. In overexpression lines, BGLC3 activity was concentrated in a microsome-enriched fraction but also was present in soluble form. Finally, both genes were generally expressed in the same cell types, although, in some cases, BGLC3 was expressed at earlier stages than BGLC1. We propose that functional specialization could explain the separate localization of both enzymes, as a membrane-bound b-glucosidase could specifically digest soluble xyloglucan without affecting the wall-bound polymer.

show abstract

“…AtFLA3-silenced Arabidopsis had abnormal pollen grains, also suggesting a function in pollen formation [24]. FLAs have also been implicated in cell-cell communication [13], shoot development [25,26], seed mucilage adherence [27], glycan stabilization [28] and in response to stresses from salt [29][30][31], cold [32] and hydrogen peroxide [33].…”

Section: Overexpression Of Ghgalt1 Promoted Cotton Fiber Development mentioning

confidence: 99%

Fasciclin-like arabinogalactan gene family in Nicotiana benthamiana: genome-wide identification, classification and expression in response to pathogens

Lai

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: Nicotiana benthamiana is widely used as a model plant to study plant-pathogen interactions. Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), participate in mediating plant growth, development and response to abiotic stress. However, the members of FLAs in N. benthamiana and their response to plant pathogens are unknown.Results: 38 NbFLAs were identified from a genome-wide study. NbFLAs could be divided into four subclasses, and their gene structure and motif composition were conserved in each subclass. NbFLAs may be regulated by cis-acting elements such as STRE and MBS, and could possibly be the targets of transcription factors like C2H2. Quantitative real time polymerase chain reaction (RT-qPCR) results showed that selected NbFLAs were differentially expressed in different tissues. All of the selected NbFLAs were significantly downregulated following infection by turnip mosaic virus (TuMV) and most of them also by Pseudomonas syringae pv tomato (Pst) strain DC3000 (Pst DC3000), suggesting possible roles in response to pathogenic infection.Conclusions: This study systematically identified FLAs in N. benthamiana, and indicates their potential roles in response to biotic stress. The identification of NbFLAs will facilitate further studies of their role in plant immunity in N. benthamiana.

show abstract

Cold acclimation is accompanied by complex responses of glycosylphosphatidylinositol (GPI)-anchored proteins in Arabidopsis

Abstract: HighlightChanges in GPI-anchored proteins in plasma membrane-, microdomain-, and apoplast-fractions suggest the involvement of GPI-anchored proteins in the acquisition of freezing tolerance.

Cited by 52 publications

References 67 publications

Root Skewing-Associated Genes Impact the Spaceflight Response of Arabidopsis thaliana

Root Skewing-Associated Genes Impact the Spaceflight Response of Arabidopsis thaliana

Soluble and Membrane-Bound β-Glucosidases Are Involved in Trimming the Xyloglucan Backbone

Fasciclin-like arabinogalactan gene family in Nicotiana benthamiana: genome-wide identification, classification and expression in response to pathogens

Contact Info

Product

Resources

About