Cold adaptation in bacteria with special focus on cellulase production and its potential application

Gupta, Santosh Kumar; Kataki, Sampriti; Chatterjee, Soumya; Prasad, Rajesh Kumar; Datta, Sibnarayan; Vairale, Mohan G.; Sharma, Sonika; Dwivedi, Sanjai K.; Gupta, Dharmendra K.

doi:10.1016/j.jclepro.2020.120351

Cited by 43 publications

(20 citation statements)

References 209 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Biotechnological exploitation of cold-active β-galactosidases . The importance and the advantage of cold-active enzymes in industrial application are known and their potentiality is reported in several reviews [ 3 , 12 , 13 ]. Cold-active β-galactosidases from psychrophilic and mesophilic organisms might play a key role in food industries in the production of lactose free products and prebiotics.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the ability of cold-active enzymes to catalyze reactions at low temperature can help to reduce the environmental impact and energy consumption of the process. Cold-active enzymes find application in detergency, waste bioremediation, molecular biology and in the medical, pharmaceutical and food industries [ 3 , 4 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cold-Active β-Galactosidases: Insight into Cold Adaptation Mechanisms and Biotechnological Exploitation

Mangiagalli

Lotti

2021

Marine Drugs

View full text Add to dashboard Cite

β-galactosidases (EC 3.2.1.23) catalyze the hydrolysis of β-galactosidic bonds in oligosaccharides and, under certain conditions, transfer a sugar moiety from a glycosyl donor to an acceptor. Cold-active β-galactosidases are identified in microorganisms endemic to permanently low-temperature environments. While mesophilic β-galactosidases are broadly studied and employed for biotechnological purposes, the cold-active enzymes are still scarcely explored, although they may prove very useful in biotechnological processes at low temperature. This review covers several issues related to cold-active β-galactosidases, including their classification, structure and molecular mechanisms of cold adaptation. Moreover, their applications are discussed, focusing on the production of lactose-free dairy products as well as on the valorization of cheese whey and the synthesis of glycosyl building blocks for the food, cosmetic and pharmaceutical industries.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cold-Active β-Galactosidases: Insight into Cold Adaptation Mechanisms and Biotechnological Exploitation

Mangiagalli

Lotti

2021

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…In addition, among psychrophilic and psychrotrophic bacteria, there are often producers of enzymes that mediate the degradation of insoluble natural biopolymers, such as cellulose and chitin. Currently, the possibility of using these enzymes as antimicrobial agents in a complex therapy directed against pathogenic bacteria that form biofilms resistant to antibiotics is actively studied [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

First Insight into the Diversity and Antibacterial Potential of Psychrophilic and Psychotrophic Microbial Communities of Abandoned Amber Quarry

et al. 2021

View full text Add to dashboard Cite

Natural habitats, including extreme ones, are potential sources of new antimicrobial compound producers, such as bacteriocins and enzymes, capable of degrading the matrix polysaccharides of bacterial biofilms. This study aimed to investigate biodiversity and evaluate the antibacterial potential of psychrophilic and psychrotrophic microbial communities of the flooded Walter amber quarry (Kaliningrad region, Russia). As a result of 16S rDNA high-throughput profiling, 127 genera of bacteria belonging to 12 phyla of bacteria were found in sediment samples: Acidobacteria sp., Actinobacteria sp., Armatimonadetes sp., Bacteroidetes sp., Chloroflexi sp., Cyanobacteria sp., Firmicutes sp., Gemmatimonadetes sp., Planctomycetes sp., Proteobacteria sp., Tenericutes sp., and Verrucomicrobia sp. The dominant bacteria groups were the families Ruminococcaceae and Lachnospiraceae, belonging to the order Clostridiales phylum Firmicutes. Analysis of enrichment cultures obtained from sediments showed the presence of antibacterial and cellulolytic activity. It seems likely that the bacteria of the studied communities are producers of antimicrobial compounds and have the potential for biotechnological use.

show abstract

“…The synthesis of compatible solutes (glycine, betaine, glycerin, trehalose, sucrose, mannitol, sorbitol) has the function of antifreeze protection and acts as a carbon and nitrogen source for microorganisms. The mass production of cold stress proteins plays a positive role in the process of cold adaptation of microorganisms 2 , 3 . Currently, more and more studies have applied multi-omics technology to explore the mechanism of bacteria adapting to the cold environment 4 , 5 .…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequencing of Bacillus sp. TK-2, analysis of its cold evolution adaptability

Shen

Zang²,

Sun³

et al. 2021

Sci Rep

View full text Add to dashboard Cite

To date, a large number of Bacillus species from different sources have been identified. However, there are few investigations on genome information and evolutionary insights of Bacillus species from cold environments. Bacillus sp. TK-2, isolated from the soil of Changbai Mountain, is a gram-positive bacterium with cold adaptation characteristics. In this study, we present the annotated complete genome sequence of Bacillus sp. TK-2. The genome comprised 5,286,177 bp with a GC content of 35.88%, 5293 protein-encoding genes, 32 rRNA, and 77 tRNA. Numerous genes related to cold adaptation were detected in the genome of Bacillus sp. TK-2, mainly involving in energy supply, regulation of cell membrane fluidity, antioxidant, and molecular chaperones. In addition, the strain TK-2 classified in the Bacillus groups was distributed on a terminal branch with Bacillus cereus A1 by Blastn and phylogenetic analysis in NCBI database. Complete genome sequences of the strain TK-2 and Bacillus cereus A1 were compared by the online tool “Average Nucleotide Identity”, showing that the average nucleotide identity of these two strains was 98.26%. In parallel, A comparative analysis of the genomes of both Bacillus sp. TK-2 and Bacillus cereus A1 was conducted. Through the analysis of core and specific genes with cd-hit, it was found that the two strains had 5691 pan gene, 4524 core gene, and 1167 specific gene clusters. Among the 624 specific gene clusters of Bacillus sp. TK-2, some cold tolerance genes were detected, which implied the unique adaptability of Bacillus sp. TK-2 in long-term low temperature environments. Importantly, enzyme-encoding genes related to the degradation of polysaccharides such as cellulose and hemicellulose were detected in the 477 CAZyme genes of this genome. This work on sequencing and bioinformatics analysis of the complete sequence of Bacillus sp. TK-2 promote the application and in-depth research of low-temperature biotechnology.

show abstract

Cold adaptation in bacteria with special focus on cellulase production and its potential application

Cited by 43 publications

References 209 publications

Cold-Active β-Galactosidases: Insight into Cold Adaptation Mechanisms and Biotechnological Exploitation

Cold-Active β-Galactosidases: Insight into Cold Adaptation Mechanisms and Biotechnological Exploitation

First Insight into the Diversity and Antibacterial Potential of Psychrophilic and Psychotrophic Microbial Communities of Abandoned Amber Quarry

Complete genome sequencing of Bacillus sp. TK-2, analysis of its cold evolution adaptability

Contact Info

Product

Resources

About