Cold adaptation of a psychrophilic chaperonin from Psychrobacter sp. and its application for heterologous protein expression

Kim, Han-Woo; Wi, Ah Ram; Jeon, Byoung Wook; Lee, Jun Hyuck; Shin, Seung Chul; Park, Hyun; Jeon, Sung-Jong

doi:10.1007/s10529-015-1860-y

Cited by 13 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kim et al ( 2015 ) co-expressed a cold active esterase together with PsyGroELS, a chaperonin from the psychrophilic bacterium Psychrobacter sp. PAMC21119, in an E. coli strain.…”

Section: Gene Cloning and Recombinant Expression Systems For Cold-actmentioning

confidence: 99%

Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes

et al. 2016

View full text Add to dashboard Cite

Cold-active enzymes constitute an attractive resource for biotechnological applications. Their high catalytic activity at temperatures below 25 • C makes them excellent biocatalysts that eliminate the need of heating processes hampering the quality, sustainability, and cost-effectiveness of industrial production. Here we provide a review of the isolation and characterization of novel cold-active enzymes from microorganisms inhabiting different environments, including a revision of the latest techniques that have been used for accomplishing these paramount tasks. We address the progress made in the overexpression and purification of cold-adapted enzymes, the evolutionary and molecular basis of their high activity at low temperatures and the experimental and computational techniques used for their identification, along with protein engineering endeavors based on these observations to improve some of the properties of cold-adapted enzymes to better suit specific applications. We finally focus on examples of the evaluation of their potential use as biocatalysts under conditions that reproduce the challenges imposed by the use of solvents and additives in industrial processes and of the successful use of cold-adapted enzymes in biotechnological and industrial applications.

show abstract

“…Kim et al ( 2015 ) co-expressed a cold active esterase together with PsyGroELS, a chaperonin from the psychrophilic bacterium Psychrobacter sp. PAMC21119, in an E. coli strain.…”

Section: Gene Cloning and Recombinant Expression Systems For Cold-actmentioning

confidence: 99%

Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In addition, variations in the electrostatic potential of psychrophilic enzymes in and around the active site tend to be a critical parameter for low-temperature activity. Interestingly in comparison to their mesophilic or thermophilic equivalents, cold-active citrate synthase (Russell et al 1998), malate dehydrogenase (Kim et al 2015), uracil-DNA glycosylase (Leiros et al 2003;Olufsen et al 2008;Raeder et al 2010), elastase (Papaleo et al 2007), and trypsin (Gorfe et al 2000;Smalås et al 2000;Brandsdal et al 2001) are distinguished by marked differences in electrostatic potential near the active site area. The enhanced positive potential at and around the binding site for oxaloacetate and the slightly diminished negative surface potential at the binding area for NADH facilitate the interaction between the oppositely charged ligands and the enzyme surface in malate dehydrogenase (Kim et al 2015).…”

Section: Cold-adapted Enzymesmentioning

confidence: 99%

Molecular Mechanisms of Cold-Adapted Microorganisms

Singh

Kumar

Sinha

et al. 2021

Survival Strategies in Cold-Adapted Microorganisms

View full text Add to dashboard Cite

Most of the ecosystems have cold environment, be it high mountains or deep oceans. The microbes residing in these ecosystems have well-developed metabolism which helps them to thrive in extreme climatic conditions. This chapter focuses on the adaptations and variations at present at molecular level in coldadapted microorganisms. The adaptations can be mimicked in industries like food, tannery, dairy, and healthcare for the betterment of mankind.

show abstract

“…Members of the genus Psychrobacter are psychrotolerant or psychrophilic bacteria, which are widely distributed in marine and terrestrial environments ( 1 – 3 ). They have been considered good sources of cold-active enzymes for industrial and environmental uses ( 4 – 6 ). Psychrobacter piscatorii strain LQ58 (=MCCC 1A10701) is an aerobic, psychrotolerant, chemoorganotrophic bacterium, isolated from a deep-sea hydrothermal vent sediment sample collected on the East Pacific Rise at a depth of 2,906 m (3.10 S, 102.55 W).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Psychrobacter piscatorii Strain LQ58, a Psychrotolerant Bacterium Isolated from a Deep-Sea Hydrothermal Vent

2016

View full text Add to dashboard Cite

show abstract

Cold adaptation of a psychrophilic chaperonin from Psychrobacter sp. and its application for heterologous protein expression

Abstract: PsyGroELS confers cold tolerance to E. coli, and shows potential as an effective co-expression system for the stable production of psychrophilic proteins.

Cited by 13 publications

References 23 publications

Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes

Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes

Molecular Mechanisms of Cold-Adapted Microorganisms

Draft Genome Sequence of Psychrobacter piscatorii Strain LQ58, a Psychrotolerant Bacterium Isolated from a Deep-Sea Hydrothermal Vent

Contact Info

Product

Resources

About