Cold air plasma improving rheumatoid arthritis via mitochondrial apoptosis pathway

Ding, Chengbiao; Ni, Leying; Liu, Qi; Zhou, Chenxu; Wang, Guomin; Chu, Paul K.; Wu, Zhengwei

doi:10.1002/btm2.10366

Cited by 7 publications

(4 citation statements)

References 58 publications

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“…Therefore, SOD and MDA is commonly used for evaluating the level of ROS. As previous research (Ding et, al. 2022), the anterior knee joint capsule was added with nine times the volume of normal saline with the proportion of weight (g): Volume (ML) = 1:9 to prepare the 10% tissue homogenate at 2500 rpm for 10 min using a commercial kit (Nanjing Jiancheng Bioengineering Institute, China).…”

Section: ) Ros Can Activate Antioxidant Enzymes and Excessive Ros Can...supporting

confidence: 66%

LIPUS attenuates knee joint capsule fibrosis and athrogenic contracture through the TGF-β1/Smad signaling pathway

Zhou

Wang

Zhou

et al. 2023

Preprint

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As one of main causes of athrogenic contracture, joint capsule fibrosis which is described as a condition with excessive deposition of collagen components and extracellular matrix (ECM) in joint capsule, is a response to long-time immobilization. The purpose of this study was to explore the effect and the underlying mechanism of low-intensity pulsed ultrasound (LIPUS) in treating knee joint capsule fibrosis. A rabbit model of knee joint capsule fibrosis induced by 6w-immobilization was employed in this study. After 6 weeks of immobilization, the degree of arthrogenic contracture and collagen density increased. Increased reactive oxygen species (ROS) levels were manifested as increased malondialdehyde (MDA) activity and decreased superoxide dismutase (SOD) content. Correspondingly, the TGF-β1/Smad signaling pathway was significantly activated, as indicated by the increased protein levels of TGF-β1, p-Smad2/Smad2, p-Smad3/Smad3 and Smad4 and the increase of p-Smad2/3 positive cells. After 2 weeks of LIPUS treatment, the degree of arthrogenic contracture and the collagen density were lower than 2 weeks of remobilizaiton. Relatively, the activity of MDA was decresed and the content of SOD was increased compared with 2 weeks of remobilizaiton. Importantly, the TGF-β1/Smad signaling pathway was significantly inhibited compared with 2 weeks of remobilizaiton. Our findings thus conclude that high level ROS and hyperactive TGF-β1/Smad signaling pathway might be one of the causes of knee joint capsule fibrosis induced by immobilization and LIPUS attenuated the severity of immobilization-induced knee joint capsule fibrosis through inhibition of the production of ROS and the activation of TGF-β1/Smad signaling pathway.

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Section: ) Ros Can Activate Antioxidant Enzymes and Excessive Ros Can...supporting

confidence: 66%

LIPUS attenuates knee joint capsule fibrosis and athrogenic contracture through the TGF-β1/Smad signaling pathway

Zhou

Wang

Zhou

et al. 2023

Preprint

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“…Our study is supported by previously published studies which demonstrating the pro-apoptotic effect of CAP on RA-FLS via the mitochondrial pathway. 16 , 19 Similarly, Hoechst staining again demonstrated the pro-apoptotic effect of cold plasma on RA-FLS.…”

Section: Discussionmentioning

confidence: 89%

“…Finally, the cells that invaded the lower layer of the microporous membrane were counted under an inverted microscope and the mean number was taken. 19 …”

Section: Methodsmentioning

confidence: 99%

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Cold Air Plasma Inhibiting Tumor-Like Biological Behavior of Rheumatoid Arthritis Fibroblast-Like Synovial Cells via G2/M Cell Cycle Arrest

Ni,

Ding,

Deng

et al. 2024

OARRR

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Background Rheumatoid arthritis fibroblast-like synovial cells (RA-FLS) have become the core effector cells for the progression of rheumatoid arthritis due to their “tumor-like cell” characteristics, such as being able to break free from growth restrictions caused by contact inhibition, promoting angiogenesis, invading surrounding tissues, and leading to uncontrolled synovial growth. In recent years, cold air plasma (CAP) has been widely recognized for its clear anticancer effect. Inspired by this, this study investigated the inhibitory effect of CAP on the tumor-like biological behavior of RA-FLS through in vitro experiments. Methods Treatment of RA-FLS with CAP at different time doses (0s, 30s, 60s, 120s). 5-ethynyl-2’-deoxyuridine (EdU) proliferation assay was used to determine the cell viability. Analysis of cell migration and invasion was performed by wound-healing assay, transwell assay and immunofluorescent staining for f-actin, respectively. Flow cytometry technique was used for analysis of cell cycle and determination of reactive oxygen species (ROS). Hoechst staining was used for analysis of cell apoptosis. Protein expression was analyzed by Western blot analysis. Results Molecular and cellular level mechanisms have revealed that CAP blocks RA-FLS in the G2/M phase by increasing intracellular reactive oxygen species (ROS), leading to increased apoptosis and significantly reduced migration and invasion ability of RA-FLS. Conclusion Overall, CAP has significant anti proliferative, migratory, and invasive effects on RA-FLS. This study reveals a new targeted treatment strategy for RA.

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Rational formulation of saponin and dexamethasone for the synergistic treatment of rheumatoid arthritis in vivo

Yue,

Liao,

Zhou

et al. 2024

Applied Materials Today

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Cold air plasma improving rheumatoid arthritis via mitochondrial apoptosis pathway

Cited by 7 publications

References 58 publications

LIPUS attenuates knee joint capsule fibrosis and athrogenic contracture through the TGF-β1/Smad signaling pathway

LIPUS attenuates knee joint capsule fibrosis and athrogenic contracture through the TGF-β1/Smad signaling pathway

Cold Air Plasma Inhibiting Tumor-Like Biological Behavior of Rheumatoid Arthritis Fibroblast-Like Synovial Cells via G2/M Cell Cycle Arrest

Rational formulation of saponin and dexamethasone for the synergistic treatment of rheumatoid arthritis in vivo

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