Cold atmospheric helium plasma jet in humid air environment

Lin, Li; Lyu, Yuan-wei; Trink, Barry; Canady, Jerome; Keidar, Michael

doi:10.1063/1.5086177

Cited by 33 publications

(25 citation statements)

References 67 publications

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“…The CAP device was manufactured at the Micro-propulsion and Nanotechnology Laboratory of the George Washington University 73 , 74 . The device was operated at a frequency of 12.5 kHz with a helium flow rate of 6.5 L per min.…”

Section: Methodsmentioning

confidence: 99%

Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells

Gjika

Pal‐Ghosh

Kirschner

et al. 2020

Sci Rep

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Cold atmospheric plasma (CAP) technology, a relatively novel technique mainly investigated as a stand-alone cancer treatment method in vivo and in vitro, is being proposed for application in conjunction with chemotherapy. In this study, we explore whether CAP, an ionized gas produced in laboratory settings and that operates at near room temperature, can enhance Temozolomide (TMZ) cytotoxicity on a glioblastoma cell line (U87MG). Temozolomide is the first line of treatment for glioblastoma, one of the most aggressive brain tumors that remains incurable despite advancements with treatment modalities. The cellular response to a single CAP treatment followed by three treatments with TMZ was monitored with a cell viability assay. According to the cell viability results, CAP treatment successfully augmented the effect of a cytotoxic TMZ dose (50 μM) and further restored the effect of a non-cytotoxic TMZ dose (10 μM). Application of CAP in conjunction TMZ increased DNA damage measured by the phosphorylation of H2AX and induced G2/M cell cycle arrest. These findings were supported by additional data indicating reduced cell migration and increased αvβ3 and αvβ5 cell surface integrin expression as a result of combined CAP–TMZ treatment. The data presented in this study serve as evidence that CAP technology can be a suitable candidate for combination therapy with existing chemotherapeutic drugs. CAP can also be investigated in future studies for sensitizing glioblastoma cells to TMZ and other drugs available in the market.

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Section: Methodsmentioning

confidence: 99%

Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells

Gjika

Pal‐Ghosh

Kirschner

et al. 2020

Sci Rep

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“…The second one comes from the plasma oscillation at GHz. Considering that the mean electron density of the CAP jet is about 10 12 cm −3 [33,34], the plasma frequency is usually between 10 GHz and 30 GHz. The frequency can be computed by, ω = n e e 2 m e ε , where ω is the plasma frequency, n e is the electron density, e is the unit charge, m e is the mass of an electron, and ε is the permittivity [35].…”

Section: Measurement Of Oes and Electromagnetic Emission From The Cap Jet Using Bone As A Barriermentioning

confidence: 99%

In Vitro and In Vivo Enhancement of Temozolomide Effect in Human Glioblastoma by Non-Invasive Application of Cold Atmospheric Plasma

Soni

Adhikari

Simonyan

et al. 2021

Cancers

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Glioblastoma (GBM) is one of the most aggressive forms of adult brain cancers and is highly resistant to treatment, with a median survival of 12–18 months after diagnosis. The poor survival is due to its infiltrative pattern of invasion into the normal brain parenchyma, the diffuse nature of its growth, and its ability to quickly grow, spread, and relapse. Temozolomide is a well-known FDA-approved alkylating chemotherapy agent used for the treatment of high-grade malignant gliomas, and it has been shown to improve overall survival. However, in most cases, the tumor relapses. In recent years, CAP has been used as an emerging technology for cancer therapy. The purpose of this study was to implement a combination therapy of CAP and TMZ to enhance the effect of TMZ and apparently sensitize GBMs. In vitro evaluations in TMZ-sensitive and resistant GBM cell lines established a CAP chemotherapy enhancement and potential sensitization effect across various ranges of CAP jet application. This was further supported with in vivo findings demonstrating that a single CAP jet applied non-invasively through the skull potentially sensitizes GBM to subsequent treatment with TMZ. Gene functional enrichment analysis further demonstrated that co-treatment with CAP and TMZ resulted in a downregulation of cell cycle pathway genes. These observations indicate that CAP can be potentially useful in sensitizing GBM to chemotherapy and for the treatment of glioblastoma as a non-invasive translational therapy.

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“…As shown in Figure 5C, these species densities are proportional to the H 2 O admixture, which agrees with the monotonic relationships revealed by previous publications. [38,48] Note that multiple publications have shown the effects and the importance of environmental and target-vicinity humidity on CAP, [48][49][50] whereas a shielding-gas technology can be applied to avoid the environmental chemical disturbance. [51][52][53]…”

Section: Plasma Chemistry Diagnosticsmentioning

confidence: 99%

Self‐Adaptive Plasma Chemistry and Intelligent Plasma Medicine

Lin

Yan

Lee

2021

Advanced Intelligent Systems

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Plasma‐based biomedical applications rely on the reactive oxygen and nitrogen species generated in cold atmospheric plasmas, where complex chemical kinetic schemes occur. The optimization of plasma medicine is thus required for each specific biomedical purpose. In the view of pharmacology, it is to optimize the active pharmaceutical ingredients. This work is thus the first attempt of such a complex task utilizing the recent development of machine learning technologies. Herein, a general method of passive plasma chemical diagnostics and optimization in real time is proposed. Based on spontaneous emission spectroscopy, an artificial neural network provides the gas chemical compositions along with other information such as temperatures. The information further passes through the second neural network which outputs the adjustments of external control inputs including energy, gas injections, and extractions to optimize the plasma chemistry.

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Cold atmospheric helium plasma jet in humid air environment

Cited by 33 publications

References 67 publications

Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells

Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells

In Vitro and In Vivo Enhancement of Temozolomide Effect in Human Glioblastoma by Non-Invasive Application of Cold Atmospheric Plasma

Self‐Adaptive Plasma Chemistry and Intelligent Plasma Medicine

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