Cold Atmospheric Plasma as a Therapeutic Tool in Medicine and Dentistry

Koga-Ito, Cristiane Yumi; Kostov, K. G.; Miranda, F. S.; Milhan, N. V.M.; Azevedo Neto, N. F.; Nascimento, F.; Pessoa, R. S.

doi:10.1007/s11090-023-10380-5

Cited by 15 publications

(7 citation statements)

References 157 publications

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“…It contains numerous shortlived chemical species such as free radicals, ions, electrons, and excited species as well as ultraviolet (UV) radiation, and electric field [2]. In the past decade, CAP has been widely used in various biomedical fields such as blood coagulation, orthopedics, skin treatment, dental cleaning, hair treatment, and sterilization [3][4][5][6][7][8][9]. The discovery of anticancer properties of CAP has led investigations into its effects on various types of tumors and their underlying mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Cold atmospheric plasma (CAP) treatment increased reactive oxygen and nitrogen species (RONS) levels in tumor samples obtained from patients with low-grade glioma

Aggarwal,

Vaid,

Visani

et al. 2024

Biomed. Phys. Eng. Express

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Low-grade gliomas (LGGs) are a heterogeneous group of tumors with an average 10-year survival rate of 40-55%. Current treatment options include chemotherapy, radiotherapy, and gross total resection (GTR) of the tumor. The extent of resection (EOR) plays an important role in improving surgical outcomes. However, the major obstacle in treating low-grade gliomas is their diffused nature and the presence of residual cancer cells at the tumor margins post resection. Cold Atmospheric Plasma (CAP) has shown to be effective in targeted killing of tumor cells in various glioma cell lines without affecting non-tumor cells through Reactive Oxygen and Nitrogen Species (RONS). However, no study on the effectiveness of CAP has been carried out in LGG tissues till date. In this study, we applied helium-based CAP on tumor tissues resected from LGG patients. Our results show that CAP is effective in promoting RONS accumulation in LGG tissues when CAP jet parameters are set at 4kV voltage, 5min treatment time and 3lpm gas flow rate. We also observed that CAP jet is more effective in thinner slice preparations of tumor as compared to thick tumor samples. Our results indicate that CAP could prove to be an effective adjunct therapy in glioma surgery to target residual cancer cells to improve surgical outcome of patients with low-grade glioma.

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Section: Introductionmentioning

confidence: 99%

Cold atmospheric plasma (CAP) treatment increased reactive oxygen and nitrogen species (RONS) levels in tumor samples obtained from patients with low-grade glioma

Aggarwal,

Vaid,

Visani

et al. 2024

Biomed. Phys. Eng. Express

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“…The plasma may also disrupt nucleotide strands through a combination of UV-induced damage and oxidative chemical effects [ 11 ]. As such, there are no known bacterial resistance mechanisms to CAP treatment and it has a wide spectrum of activities [ 12 , 13 ]. CAP has also been observed in a study carried out by Scholtz et al, 2021, to have antimicrobial properties against biofilm structures [ 14 ], though the biofilm does provide significant protection to the bacteria contained within [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Cold Plasma Deposition of Tobramycin as an Approach to Localized Antibiotic Delivery to Combat Biofilm Formation

Olayiwola,

O’Neill,

Frewen

et al. 2024

Pathogens

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Hospital-acquired infections (HAIs) remain a significant factor in hospitals, with implant surfaces often becoming contaminated by highly resistant strains of bacteria. Recent studies have shown that electrical plasma discharges can reduce bacterial load on surfaces, and this approach may help augment traditional antibiotic treatments. To investigate this, a cold atmospheric plasma was used to deposit tobramycin sulphate onto various surfaces, and the bacterial growth rate of K. pneumoniae in its planktonic and biofilm form was observed to probe the interactions between the plasma discharge and the antibiotic and to determine if there were any synergistic effects on the growth rate. The plasma-deposited tobramycin was still active after passing through the plasma field and being deposited onto titanium or polystyrene. This led to the significant inhibition of K. pneumoniae, with predictable antibiotic dose dependence. Separate studies have shown that the plasma treatment of the biofilm had a weak antimicrobial effect and reduced the amount of biofilm by around 50%. Combining a plasma pre-treatment on exposed biofilm followed by deposited tobramycin application proved to be somewhat effective in further reducing biofilm growth. The plasma discharge pre-treatment produced a further reduction in the biofilm load beyond that expected from just the antibiotic alone. However, the effect was not additive, and the results suggest that a complex interaction between plasma and antibiotic may be at play, with increasing plasma power producing a non-linear effect. This study may contribute to the treatment of infected surgical sites, with the coating of biomaterial surfaces with antibiotics reducing overall antibiotic use through the targeted delivery of therapeutics.

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“…The variety of methods for creating plasma and the ability to vary its parameters over a wide range determine the distribution of plasma in a large number of technological fields [1]. Thus, physical plasma is already actively used in the modification of surfaces and liquids [2][3][4], the purification of gas and liquid media [5][6][7][8], the synthesis of micro-and nanomaterials [9], medicine [10][11][12][13][14], agriculture [15][16][17][18][19][20], food processing [21,22], etc., and is also planned for use in the future in areas such as energy industry (controlled fusion), space agriculture, and space medicine [23].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Decontamination of Water-Containing Objects Using Piezoelectric Direct Discharge Plasma and Plasma Jet

Konchekov,

Gudkova,

Burmistrov

et al. 2024

Biomolecules

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Cold atmospheric plasma has become a widespread tool in bacterial decontamination, harnessing reactive oxygen and nitrogen species to neutralize bacteria on surfaces and in the air. This technology is often employed in healthcare, food processing, water treatment, etc. One of the most energy-efficient and universal methods for creating cold atmospheric plasma is the initiation of a piezoelectric direct discharge. The article presents a study of the bactericidal effect of piezoelectric direct discharge plasma generated using the multifunctional source “CAPKO”. This device allows for the modification of the method of plasma generation “on the fly” by replacing a unit (cap) on the working device. The results of the generation of reactive oxygen and nitrogen species in a buffer solution in the modes of direct discharge in air and a plasma jet with an argon flow are presented. The bactericidal effect of these types of plasma against the bacteria E. coli BL21 (DE3) was studied. The issues of scaling the treatment technique are considered.

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Cold Atmospheric Plasma as a Therapeutic Tool in Medicine and Dentistry

Cited by 15 publications

References 157 publications

Cold atmospheric plasma (CAP) treatment increased reactive oxygen and nitrogen species (RONS) levels in tumor samples obtained from patients with low-grade glioma

Cold atmospheric plasma (CAP) treatment increased reactive oxygen and nitrogen species (RONS) levels in tumor samples obtained from patients with low-grade glioma

Cold Plasma Deposition of Tobramycin as an Approach to Localized Antibiotic Delivery to Combat Biofilm Formation

Bacterial Decontamination of Water-Containing Objects Using Piezoelectric Direct Discharge Plasma and Plasma Jet

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