Cold atmospheric plasma treatment of melanoma and glioblastoma cancer cells

Vermeylen, Stijn; Waele, Jorrit De; Vanuytsel, Steven; Backer, Joey De; Paal, Jonas Van der; Ramakers, Marleen; Leyssens, Karen; Marcq, Elly; Audenaerde, Jonas R.M. Van; Smits, Evelien; Dewilde, Sylvia; Bogaerts, Annemie

doi:10.1002/ppap.201600116

Cited by 61 publications

(66 citation statements)

References 77 publications

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“…Jurkat cells were treated by plasma from the nspDBD [33] or the COST-Jet [34] (He + 0.6% O 2 ), using conditions described in previous studies (Table 1). In these studies, reduced viability was correlated with frequency for the nspDBD and treatment time for the COST-Jet; thus, we treated from 30-75 Hz with the nspDBD and up to 5 min with the COST-Jet.…”

Section: Cost-jet and Nspdbd Treatment Cause Comparable Reductions Inmentioning

confidence: 99%

GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment

et al. 2020

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This study investigated the use of glutathione as a marker to establish a correlation between plasma parameters and the resultant liquid chemistry from two distinct sources to predefined biological outcomes. Two different plasma sources were operated at parameters that resulted in similar biological responses: cell viability, mitochondrial activity, and the cell surface display of calreticulin. Specific glutathione modifications appeared to be associated with biological responses elicited by plasma. These modifications were more pronounced with increased treatment time for the European Cooperation in Science and Technology Reference Microplasma Jet (COST-Jet) and increased frequency for the dielectric barrier discharge and were correlated with more potent biological responses. No correlations were found when cells or glutathione were exposed to exogenously added long-lived species alone. This implied that short-lived species and other plasma components were required for the induction of cellular responses, as well as glutathione modifications. These results showed that comparisons of medical plasma sources could not rely on measurements of long-lived chemical species; rather, modifications of biomolecules (such as glutathione) might be better predictors of cellular responses to plasma exposure.

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Section: Cost-jet and Nspdbd Treatment Cause Comparable Reductions Inmentioning

confidence: 99%

GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment

et al. 2020

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“…Vermeylen et al used the COST jet operated with the He+O 2 feed gas to study the effects of various parameters on the treatment of different melanoma and glioblastoma cell lines [51]. While the viability of all cell types was reduced as a result of plasma treatment, some appeared to be more susceptible to it than the others.…”

Section: Identifying Optimal Parameters For Anti-cancer Treatmentsmentioning

confidence: 99%

“…The COST jet was also used in the comparative use of plasma-treated media as opposed to the direct plasma treatment [51]. This addresses one of the main reasons for the scepticism towards plasma medicine.…”

Section: Identifying Optimal Parameters For Anti-cancer Treatmentsmentioning

confidence: 99%

Applications of the COST Plasma Jet: More than a Reference Standard

Gorbanev

Golda

Gathen

et al. 2019

Plasma

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The rapid advances in the field of cold plasma research led to the development of many plasma jets for various purposes. The COST plasma jet was created to set a comparison standard between different groups in Europe and the world. Its physical and chemical properties are well studied, and diagnostics procedures are developed and benchmarked using this jet. In recent years, it has been used for various research purposes. Here, we present a brief overview of the reported applications of the COST plasma jet. Additionally, we discuss the chemistry of the plasma-liquid systems with this plasma jet, and the properties that make it an indispensable system for plasma research.

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“…Potential medical applications of NEAPP have been reviewed elsewhere, and dramatically expanded due to indirect treatments with NEAPP‐activated liquid medium. In the indirect treatments, cultivation, or incubation of cells in cell culture medium irradiated with NEAPP, called plasma‐activated medium (PAM), exhibits anti‐tumor effects against various types of cancer cells …”

Section: Introductionmentioning

confidence: 99%

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

Furuta

Kurake

Ishikawa

et al. 2017

Plasma Process Polym

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A mechanism of the cytotoxicity of plasma-activated medium (PAM) is revealed by examining the intracellular effects of reactive oxygen nitrogen species (RONS) and lipid oxidation. PAM is cell culture medium activated by irradiation using non-equilibrium atmospheric-pressure plasma using pure Ar gas in ambient air. Extracellular RONS in PAM induced the apoptotic death of HeLa cells. Temporal changes in intracellular RONS, such as ONOO − , NO, and O À 2 , were analyzed. Intracellular RONS generation in HeLa cells following incubation in PAM triggered activation of the caspase cascade pathway and lipid peroxidation of intracellular membranes to induce apoptosis. K E Y W O R D S non-equilibrium atmospheric-pressure plasma, plasma medicine plasma-activated medium (PAM), reactive oxygen and nitrogen species (RONS)

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Cold atmospheric plasma treatment of melanoma and glioblastoma cancer cells

Cited by 61 publications

References 77 publications

GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment

GSH Modification as a Marker for Plasma Source and Biological Response Comparison to Plasma Treatment

Applications of the COST Plasma Jet: More than a Reference Standard

Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma-activated medium

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