Cold atmospheric pressure plasmas exhibit antimicrobial properties against critical bacteria and yeast species

Wiegand, Cornelia; Fink, Sarah; Uc, Hipler; Beier, Oliver; Horn, Kerstin; Pfuch, Andreas; Schimanski, Arnd; Grünler, Bernd

doi:10.12968/jowc.2017.26.8.462

Cited by 18 publications

(8 citation statements)

References 37 publications

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“…The antimicrobial action of gas plasma for planktonic and biofilm 229 microorganisms, including ESKAPE pathogens, is wellestablished. [230][231][232][233] Gas plasma is created at the point of care by a high-voltage electric arc in the chosen gas and kills organisms by the action of reactive oxygen species. 234,235 Gas plasma may stimulate healing 236 through enhanced angiogenesis 237 and local tissue perfusion.…”

Section: Advanced Therapy Options For Controlling Inflammation and Bioburdenmentioning

confidence: 99%

Implementing TIMERS: the race against hard-to-heal wounds

et al. 2019

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Section: Advanced Therapy Options For Controlling Inflammation and Bioburdenmentioning

confidence: 99%

Implementing TIMERS: the race against hard-to-heal wounds

et al. 2019

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“…The microbicidal effect of CAP was observed in vitro [256,257,258,259,260,261] on skin and chronic wounds, and exceeds the effectiveness of CHD, PVP-I, and PHMB. In a 3D epidermis model, CAP displayed dose- and time-dependent compatibility [256]; P. aeruginosa was inactivated without destroying the structure of the epidermis.…”

Section: Future Perspectivesmentioning

confidence: 99%

Consensus on Wound Antisepsis: Update 2018

Krämer

Dissemond²,

Kim³

et al. 2017

Skin Pharmacol Physiol

256

215

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Wound antisepsis has undergone a renaissance due to the introduction of highly effective wound-compatible antimicrobial agents and the spread of multidrug-resistant organisms (MDROs). However, a strict indication must be set for the application of these agents. An infected or critically colonized wound must be treated antiseptically. In addition, systemic antibiotic therapy is required in case the infection spreads. If applied preventively, the Wounds-at-Risk Score allows an assessment of the risk for infection and thus appropriateness of the indication. The content of this updated consensus recommendation still largely consists of discussing properties of octenidine dihydrochloride (OCT), polihexanide, and iodophores. The evaluations of hypochlorite, taurolidine, and silver ions have been updated. For critically colonized and infected chronic wounds as well as for burns, polihexanide is classified as the active agent of choice. The combination 0.1% OCT/phenoxyethanol (PE) solution is suitable for acute, contaminated, and traumatic wounds, including MRSA-colonized wounds due to its deep action. For chronic wounds, preparations with 0.05% OCT are preferable. For bite, stab/puncture, and gunshot wounds, polyvinylpyrrolidone (PVP)-iodine is the first choice, while polihexanide and hypochlorite are superior to PVP-iodine for the treatment of contaminated acute and chronic wounds. For the decolonization of wounds colonized or infected with MDROs, the combination of OCT/PE is preferred. For peritoneal rinsing or rinsing of other cavities with a lack of drainage potential as well as the risk of central nervous system exposure, hypochlorite is the superior active agent. Silver-sulfadiazine is classified as dispensable, while dyes, organic mercury compounds, and hydrogen peroxide alone are classified as obsolete. As promising prospects, acetic acid, the combination of negative pressure wound therapy with the instillation of antiseptics (NPWTi), and cold atmospheric plasma are also subjects of this assessment.

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“…Unlike conventional therapy, the occurrence of microbial resistance to cold plasma is unlikely due to its multiple forms of action and diversity of active agents [ 36 , 37 , 38 ]. Reactive species produced by CAP can induce oxidative damage to the biofilm during plasma exposure and, in this case, no specific interaction is required to induce the antifungal effect [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms

et al. 2021

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The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.

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Cold atmospheric pressure plasmas exhibit antimicrobial properties against critical bacteria and yeast species

Abstract: The study showed that CAPPs demonstrate strong bactericidal and fungicidal properties in vitro. The selective application of CAPPs for the treatment of wound infections may offer a promising supplementary tool alongside current therapies.

Cited by 18 publications

References 37 publications

Implementing TIMERS: the race against hard-to-heal wounds

Implementing TIMERS: the race against hard-to-heal wounds

Consensus on Wound Antisepsis: Update 2018

Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms

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