Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Fki, Lotfi; Bouaziz, N.; Chkir, O.; Benjemaa-Masmoudi, Raja; Rival, Alain; Swennen, Rony; Drira, Noureddine; Panis, Bart

doi:10.1007/s10535-012-0284-y

Cited by 23 publications

(17 citation statements)

References 21 publications

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“…Khalas) cell suspension. Fki et al (2011b) reported the successful cryopreservation method for proembryogenic masses (PEMs) of date palm variety Barhee and the morphology of the regenerated plants confirmed the stability of the clonal material. Furthermore, Fki et al (2013) reported the regeneration of cryopreserved callogenic meristems of date palm cv.…”

Section: Cryopreservationmentioning

confidence: 88%

“…Research has showed that somaclonal variation frequency is depend on the age of the tissue cultured date palm (Saker et al, 2000). The high concentration of 2,4-D induced 25% somaclonal variation and observed change in the leaf morphology and also cause poor flower pollination leads to low quality date fruit (Fki et al, 2011b). To reduce the risk of somaclonal variation researchers suggest using juvenile explants, low auxin concentration, especially 2,4-D and minimum numbers of subcultures (El Hadrami et al, 2011;Fki et al, 2011aFki et al, , 2011bBi, 2012).…”

Section: Somaclonal Variationmentioning

confidence: 99%

“…In addition to providing a means for rapid clonal propagation of date palm (Khierallah;Bader, 2007), tissue culture techniques can be utilized for the production of synthetic seeds , cell suspension culture (Othmani et al, 2009b), cryopreservation (Fki et al, 2013), somaclonal variation introduces stress tolerant, disease resistance and with high quality fruits El Hadrami, 2009;Jain, 2001), the production of secondary metabolites (ElSharabasy, 2004) and commercial production of date palm gives considerable profit to both public-sector and private agencies (Zaid;El-Korchi;Visser, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Date palm micropropagation: Advances and applications

Al-Khayri

Naik

2017

Ciênc. agrotec.

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Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature inflorescences of adult trees were utilized as an alternative nondestructive source of explants. In addition to the nature of the explant used, successful plant regeneration depends on the cultivar, composition of the culture medium and physical status. Challenges of date palm micropropagation include long in vitro cycle, latent contamination, browning, somaclonal variation as well as ex vitro acclimatization and transplanting. A remarkable amount of research investigating these factors has led to optimized protocols for the micropropagation of numerous commercially important cultivars. This has encouraged the development of several international commercial tissue culture laboratories. Molecular characterization provides an assurance of genetic conformity of regenerated plantlets, a key feature for commercial production. This article describes date palm micropropagation protocols and also discusses recent achievements with respect to somaclonal variation, molecular markers, cryopreservation and future prospects.Index terms: Cryopreservation; somatic embryogenesis; somaclonal variation; organogenesis; molecular marker. RESUMOA tamareira (Phoenix dactylifera L.) é uma arvore frutífera adaptada à condições climáticas adversas predominantemente em regiões áridas do Oriente Médio e Norte Africano. As tâmaras possuem vários componentes químicos com alto valor medicinal e nutricional. A propagação tradicional por estacas não é suficiente para satisfazer a demanda por mudas e assim, a micropropagação apresenta-se como uma alternativa eficiente para a produção de mudas em larga escala. Embriogênese somática e organogênese, tanto direta quanto indireta via calos, tem sido usada para obter a regeneração in vitro de tamareira. O inicio do cultivo in vitro normalmente utiliza meristemas excisados de brotações jovens. Recentemente, inflorescências imaturas de árvores adultas são usadas como fonte alternativa de explantes não destrutiva. Além da origem do explante, o sucesso da regenerção depende do cultivar, da composição do meio de cultura e de condições físicas. Desafios na micropropagação de tamareira incluem um longo ciclo in vitro, contaminação, escurecimento do tecido, variação somaclonal além do enraizamento e aclimatização ex vitro. Diversos estudos investigando esses fatore...

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Section: Cryopreservationmentioning

confidence: 88%

Section: Somaclonal Variationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Date palm micropropagation: Advances and applications

Al-Khayri

Naik

2017

Ciênc. agrotec.

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“…Successful cryopreservation was observed in shoot apices of Rabdosia rubescens encapsulated and precultured in MS culture medium with 0.4 M sucrose, in combination with 2.0 M glycerol and dehydrated for 1 hour in silica gel, reaching a water content of 21% and a recovery rate of 85% (Ai, Lu, & Song, 2012). Encapsulationvitrification of apical meristems of date palm (Phoenix dactylifera L) in PVS2 for 15 minutes and immersion in LN, ensured increased survival and recovery of explants (40%) (Fki et al, 2013). Shoot apices of Vitis vinifera encapsulated were pre-cultured in half-strength MS medium supplemented with increasing concentrations of sucrose (0.25-1.0 M) and then dehydrated in an airflow hood for different periods, in order to determine the optimum capsules dehydration time (Wang, Tanne, Arav, & Gafny, 2000).…”

Section: Encapsulation-dehydrationmentioning

confidence: 99%

<b>Behavior of lateral buds of <i>Hancornia speciosa</i> after cryopreservation by encapsulation-vitrification

Prudente

Paiva

Nery

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study's objective was to assess the behavior of lateral bud's regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants.Keywords: Mangabeira, recalcitrant species, long-term conservation, dehydration.Comportamento de gemas laterais de Hancornia speciosa após criopreservação por encapsulamento-vitrificação RESUMO. Hancornia speciosa é uma frutífera do Cerrado com elevado potencial econômico. Entretanto, o extrativismo desordenado causou a redução populacional em sua área endêmica. Além disso, a recalcitrância da semente afeta negativamente sua conservação convencional. Buscando alternativas de conservação para essa espécie, objetivou-se avaliar a regeneração das gemas laterais após a técnica de encapsulamento-vitrificação. Cápsulas de alginato de sódio contendo gemas laterais foram pré-cultivadas em meio WPM acrescido de 1,0 M de glicerol e, posteriormente, imersas em diferentes concentrações de sacarose (0,3; 0,75 e 1,0 M) por 24 ou 48 horas. As cápsulas foram submetidas à desidratação em sílica gel ou em fluxo laminar por 0, 1, 2 e 3 horas antes de sua incubação em diferentes tempos de PVS2 (0, 15, 30, 60 e 120 minutos). Elevada porcentagem de regeneração de gemas laterais foi observada após a criopreservação de cápsulas tratadas com 0,75 M de sacarose + 1,0 M de glicerol por 24 horas, associado com a desidratação em fluxo laminar (1 hora) e imersão em PVS2 (15 minutos). O encapsulamento-vitrificação é eficiente para a conservação de longo prazo e permite a obtençao de altas taxas de sobrevivência após a criopreservação de explantes sensíveis (gemas laterais) de Hancornia speciosa.Palavras-chave: Mangabeira, espécie recalcitrante, conservação em longo prazo, desidratação.

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“…Biotechnology has been used to propagate, improve, and preserve plant genetic resources [1][2][3][4]. In date palm (Phoenix dactylifera L.), biotechnological techniques have already been employed for in vitro propagation [5,6].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Cryopreservation of Date Palm Caulogenic Meristems

Fki

Chkir

Kriaâ

et al. 2017

Methods in Molecular Biology

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Cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. In this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free MS medium. The positive impact of sucrose preculture and cold hardening treatments on survival rates is significant. Regeneration rates obtained with standard vitrification, encapsulationvitrification, and droplet-vitrification protocols can reach 30, 40, and 70%, respectively. All regenerated plants from non-cryopreserved or cryopreserved explants don't show morphological variation by maintaining genetic integrity without adverse effect of cryogenic treatment. Cryopreservation of date palm vitro-cultures enables commercial tissue culture laboratories to move to large-scale propagation from cryopreserved cell lines producing true-to-type plants after clonal field-testing trials. When comparing the cost of cryostorage and in-field conservation of date palm cultivars, tissue cryopreservation is the most cost-effective. Moreover, many of the risks linked to field conservation like erosion due to climatic, edaphic, and phytopathologic constraints are circumvented.

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Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Cited by 23 publications

References 21 publications

Date palm micropropagation: Advances and applications

Date palm micropropagation: Advances and applications

<b>Behavior of lateral buds of <i>Hancornia speciosa</i> after cryopreservation by encapsulation-vitrification

In Vitro Cryopreservation of Date Palm Caulogenic Meristems

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