Cold-induced injury to lung epithelial cells can be inhibited by iron chelators — implications for lung preservation☆

Pizanis, Nikolaus; Gillner, Sebastian; Kamler, Markus; Groot, Herbert de; Jakob, Heinz; Rauen, Ursula

doi:10.1016/j.ejcts.2011.01.052

Cited by 22 publications

(25 citation statements)

References 27 publications

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“…Although storage of those vessels induces injury, mechanisms of injury due to cold storage have only recently been extensively studied. Injury due to cold storage and prevention from injury have already been exhibited for corneal cells, cultured endothelial cells, hepatocytes, rat mesenteric artery, segments of rat and pig aorta and lung epithelial cells [1][2][3]. The same is true for the human internal thoracic artery [4].…”

Section: Introductionmentioning

confidence: 56%

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Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Schipke¹

2016

AVS

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ObjectiveGreat Saphenous Veins (SVs) are widely used in coronary and in non-coronary surgery, and the need for vascular transplants is increasing. In older pre¬servation solutions, hypothermia leads to damage induced by reactive oxygen species. Iron chelators in the newer Tissue Protection (TiProtec) solution shall par¬ticularly protect vessels by preventing from that damage.It was the aim to compare three preservation solutions to maintain function and viability of human SVs. MethodsVein segments were randomly assigned to Krebs-Henseleit Buffer (KHB), Viaspan (UW), or TiProtec. Contraction/relaxation in organ baths was assessed at 3, 24, 48 and 72h after removal. The protocol included three steps: receptor-independent contraction (KCl: 80μM), receptor-dependent contraction (PE: ≤30μM), endothelium-dependent relaxation (ACh: ≤20μM). Morphology: Vein segments were incubated with propidium iodide and 5-chloromethylfluorescein diacetate. Viability was assessed with fluorescence using laser scanning microscopy. ResultsKHB was inappropriate after only 3h. In TiProtec, SVs contraction/relaxation had increased further at 72h, but not in both other groups. More necrosis was found in KHB SVs than in UW or TiProtec at 3h. More viable cells were found in UW and in TiProtec SVs on day-1, while only few cells were viable in KHB on day-10. Summary and conclusionEven short storage of SVs in KHB is deleterious. UW preserved func¬tion better over time. The best functional and morphological results yielded TiProtec over time, and the amount of viable cells correlated with function. TiProtec seems a promising alternative for short-and mid-term hypothermic vein preservation.

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Section: Introductionmentioning

confidence: 56%

“…The iron mediates the formation of Reactive Oxygen Species (ROS) which in turn lead to lipid peroxidation, changes in mitochondria, and finally to cell death [10]. The rewarming when the tissue is transplanted exacerbates this process while iron chelators were shown to ameliorate the damage [1].…”

Section: Introductionmentioning

confidence: 99%

Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Schipke¹

2016

AVS

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“…Lung epithelial cells have been identified in ROS production induced by various stimulants (Faux et al 2009;Rosanna and Salvatore 2012). It has been reported that lung epithelial cells might increase ROS production after the cells were kept at 4°C for varying periods in medium and then rewarmed (for 3 h) (Pizanis et al 2011). However, the mechanism of the ROS production in pulmonary epithelial cells induced by rapid cold stress is still unclear.…”

Section: Resultsmentioning

confidence: 99%

“…Cold temperature exposure can cause respiratory responses such as cough, bronchoconstriction, and mucosal secretion (Giesbrecht 1995;Koskela 2007). In addition, it has been reported cold-induced injury to lung epithelial cells is associated to ROS formation (Pizanis et al 2011). A recent study showed that cold-sensing transient receptor potential subfamily member 1 (TRPA1) was expressed in the alveolar epithelial cells which increased release of IL-8 chemokine in response to activation of the TRPA1 (Mukhopadhyay et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Cold stress increases reactive oxygen species formation via TRPA1 activation in A549 cells

Sun

Wang

Cao

et al. 2016

Cell Stress and Chaperones

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Reactive oxygen species (ROS) are responsible for lung damage during inhalation of cold air. However, the mechanism of the ROS production induced by cold stress in the lung is still unclear. In this work, we measured the changes of ROS and the cytosolic Ca 2+ concentration ([Ca 2+ ] c ) in A549 cell. We observed that cold stress (from 20 to 5°C) exposure of A549 cell resulted in an increase of ROS and [Ca 2+ ] c , which was completely attenuated by removing Ca 2+ from medium. Further experiments showed that cold-sensing transient receptor potential subfamily member 1 (TRPA1) agonist (allyl isothiocyanate, AITC) increased the production of ROS and the level of [Ca 2+ ] c in A549 cell. Moreover, HC-030031, a TRPA1 selective antagonist, significantly inhibited the enhanced ROS and [Ca 2+ ] c induced by AITC or cold stimulation, respectively. Taken together, these data demonstrated that TRPA1 activation played an important role in the enhanced production of ROS induced by cold stress in A549 cell.

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“…Inactivation of the Na+/K+ ATPase pump allows the accumulation of calcium, sodium and water within the cell causing cellular swelling [15]. The binding of transition metals such as iron to their carrier proteins (transferrin, ferritin) is also inhibited which increases the intracellular concentration of free iron [16,17]. This is a strong catalyst for the generation of oxygen free radicals which promotes the production of other free radicals [14].…”

Section: Cold Ischaemic Injurymentioning

confidence: 99%

Cold Ischaemic Injury in Kidney Transplantation

Hosgood¹,

Hunter²,

Nicholson³

2012

Current Concepts in Kidney Transplantation

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Cold-induced injury to lung epithelial cells can be inhibited by iron chelators — implications for lung preservation☆

Cited by 22 publications

References 27 publications

Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Cold stress increases reactive oxygen species formation via TRPA1 activation in A549 cells

Cold Ischaemic Injury in Kidney Transplantation

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