Cold-inducible RNA binding protein is required for the expression of adhesion molecules and embryonic cell movement in Xenopus laevis

Peng, Ying; Yang, Pai Hao; Tanner, Julian A.; Huang, Jian; Li, Ming; Lee, Henry F.; Xu, Ren; Kung, Hsiang Fu; Lin, Marie C.M.

doi:10.1016/j.bbrc.2006.03.086

Cited by 24 publications

(29 citation statements)

References 19 publications

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“…Our observation that CIRP is elevated in breast cancer contrasts with a reported decrease in CIRP in endometrial cancer 22. CIRP has been shown to play roles in maintenance of normal cellular functions and morphogenesis, biological rhythms, suppression of cell growth induced by cold stress, and protection against apoptosis 14, 16, 19–22. Based on this, one explanation for the apparent differences in CIRP levels in breast and endometrial cancers is that CIRP may play different roles depending on the types, stress responses, and physiological state of tissues and cells.…”

Section: Discussionmentioning

confidence: 63%

“…It mediates suppression of cell growth with prolongation of G 1 phase 14, and contributes to the suppression of apoptosis induced by tumor necrosis factor‐α 19. CIRP also contributes to the maintenance of normal cellular function 20, 21. CIRP is suggested to participate in cell cycle regulation of normal human endometrium and loss of its expression may be involved in endometrial carcinogenesis 22.…”

Section: Introductionmentioning

confidence: 99%

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Cold‐inducible RNA‐binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancer

Guo

Hartley

2009

Molecular Carcinogenesis

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The cell cycle regulator cyclin E1 is aberrantly expressed in a variety of human cancers. In breast cancer, elevated cyclin E1 correlates with poor outcome, as do high cytoplasmic levels of the stress-induced RNA binding protein Human antigen R (HuR). We showed previously that increased cytoplasmic HuR elevates cyclin E1 in MCF-7 breast cancer cells by stabilizing its mRNA. We show here that cold-inducible RNA binding protein (CIRP) co-regulates cyclin E1 with HuR in breast cancer cells. CIRP had been shown to interact with HuR in Xenopus laevis oocytes and to be decreased in endometrial cancer. To investigate if human CIRP and HuR co-regulate cyclin E1, HuR and CIRP levels were altered in MCF-7 cells and effects on cyclin E1 assessed. Altering HuR expression resulted in a reciprocal change in CIRP expression, while altering CIRP expression resulted in corresponding changes in HuR and cyclin E1 expression. CIRP and HuR co-precipitated in the presence of RNA and CIRP enhanced HuR binding to the cyclin E1 mRNA and increased cyclin E1 mRNA stability. CIRP co-localized with HuR predominantly in the nucleus, but also in discrete cytoplasmic foci identified as stress granules. CIRP overexpression increased the number of HuR-containing stress granules, while its knockdown decreased them. Our results suggest that CIRP positively regulates HuR, ultimately resulting in increased protein synthesis of at least one of its targets.

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Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Cold‐inducible RNA‐binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancer

Guo

Hartley

2009

Molecular Carcinogenesis

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“…Thus, we hypothesized that it is a positive regulator for cell proliferation. Maternal Cirp is specifically required for embryonic kidney formation in Xenopus laevis (Peng et al, ). Similarly, the present study showed that Cirp could regulate the proliferation of BHK cells.…”

Section: Discussionmentioning

confidence: 99%

“…Aside from relieving stress, Cirp is also involved in cell proliferation (Nishiyama et al, ; Sakurai et al, ), development (Peng et al, ; van Venrooy et al, ; Masuda et al, ), circadian modulation (Morf et al, ) under physiological conditions, and tumor formation and progression (Artero‐C et al, ; Zeng et al, ). Cirp was found to be a Tcf‐3‐specific target gene that regulates neural development in Xenopus (van Venrooy et al, ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of gene expression profiles reveals the regulatory network of cold‐inducible RNA‐binding protein mediating the growth of BHK‐21 cells

Tang

Wang

Lan

et al. 2015

Cell Biology International

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Cold-inducible RNA-binding protein (Cirp), the first cold-shock protein identified in mammals, is a sensor protein whose expression increases in response to stress. Recent reports have shown that Cirp is involved in cell proliferation, development, circadian modulation under physiological conditions, and tumor formation and progression. However, the molecular mechanisms underlying the activities of Cirp in the mammalian kidney cells remain unclear. In this study, we constructed BHK-21cells overexpressing Cirp (Cirp + BHK-21) knockdown BHK-21 cells (Cirp - BHK-21) to investigate the function of Cirp in cell proliferation. We analyzed the gene expression of Cirp - BHK-21 cells using genome-wide expression microarrays to explore the molecular mechanism of Cirp action. We found that (1) Cirp overexpression significantly enhanced cell proliferation, whereas Cirp knockdown dramatically reduced cell proliferation, suggesting that Cirp is a positive regulator of BHK-21 cell proliferation. (2) Differentially expressed genes in Cirp - BHK-21 and control cells were shown to be involved in many biological processes. (3) Pathway analysis showed that five enriched pathways, namely, Focal adhesion, Mapk, Wnt, Apoptosis, and Cancer-related signaling pathways, were identified as central pathway networks regulated by Cirp. These results can provide new insights into the molecular mechanisms of Cirp function.

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“…It has been reported that CIRP participates in many processes during the developmental stages (Peng et al, 2006;Tanaka et al, 2000;van Venrooy et al, 2008). Xenopus CIRP was found to be expressed transiently in developing pronephros and neural tissues (Uochi and Asashima, 1998).…”

Section: The Expression Pattern Of Pocirp Duringmentioning

confidence: 99%

Molecular cloning, expression pattern, and 3D structural prediction of the cold inducible RNA-binding protein (CIRP) in Japanese flounder (Paralichthys olivaceus)

Yang

Gao

et al. 2015

J. Ocean Univ. China

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Cold-inducible RNA-binding protein (CIRP) is a kind of RNA binding proteins that plays important roles in many physiological processes. The CIRP has been widely studied in mammals and amphibians since it was first cloned from mammals. On the contrary, there are little reports in teleosts. In this study, the PoCIRP gene of the Japanese flounder was cloned and sequenced. The genomic sequence consists of seven exons and six introns. The putative PoCIRP protein of flounder was 198 amino acid residues long containing the RNA recognition motif (RRM). Phylogenetic analysis showed that the flounder PoCIRP is highly conserved with other teleost CIRPs. The 5' flanking sequence was cloned by genome walking and many transcription factor binding sites were identified. There is a CpGs region located in promoter and exon I region and the methylation state is low. Quantitative real-time PCR analysis uncovered that PoCIRP gene was widely expressed in adult tissues with the highest expression level in the ovary. The mRNA of the PoCIRP was maternally deposited and the expression level of the gene was regulated up during the gastrula and neurula stages. In order to gain the information how the protein interacts with mRNA, we performed the modeling of the 3D structure of the flounder PoCIRP. The results showed a cleft existing the surface of the molecular. Taken together, the results indicate that the CIRP is a multifunctional molecular in teleosts and the findings about the structure provide valuable information for understanding the basis of this protein's function.

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Cold-inducible RNA binding protein is required for the expression of adhesion molecules and embryonic cell movement in Xenopus laevis

Cited by 24 publications

References 19 publications

Cold‐inducible RNA‐binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancer

Cold‐inducible RNA‐binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancer

Analysis of gene expression profiles reveals the regulatory network of cold‐inducible RNA‐binding protein mediating the growth of BHK‐21 cells

Molecular cloning, expression pattern, and 3D structural prediction of the cold inducible RNA-binding protein (CIRP) in Japanese flounder (Paralichthys olivaceus)

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