Cold Physical Plasmas in the Field of Hygiene—Relevance, Significance, and Future Applications

Krämer, Axel; Bekeschus, Sander; Matthes, Rutger; Bender, Claudia; Stope, Matthias B.; Napp, Matthias; Lademann, Olaf; Lademann, Jürgen; Weltmann, Klaus‐Dieter; Schauer, Frieder

doi:10.1002/ppap.201500170

Cited by 54 publications

(43 citation statements)

References 295 publications

(270 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Murine wound healing in excisional wound models is largely mediated via muscle contraction, whereas our ear wounds provide a valid dermal full‐thickness model that heals by re‐epithelization like in humans . These results support the findings of previous studies where (i) accelerated healing of chronic wounds in domestic animals and (ii) antiseptic efficacy and pro‐healing properties in humans were successfully demonstrated. Moreover, wound healing was independent from antiseptic plasma effects.…”

Section: Resultssupporting

confidence: 87%

A cold plasma jet accelerates wound healing in a murine model of full‐thickness skin wounds

Schmidt

Bekeschus

Wende

et al. 2017

Experimental Dermatology

Self Cite

203

140

View full text Add to dashboard Cite

Cold plasma has been successfully applied in several fields of medicine that require, for example, pathogen inactivation, implant functionalization or alteration of cellular activity. Previous studies have provided evidence that plasma supports the healing of wounds owing to its beneficial mixtures of reactive species and modulation of inflammation in cells and tissues. To investigate the wound healing activity of an atmospheric pressure plasma jet in vivo, we examined the cold plasma's efficacy on dermal regeneration in a murine model of dermal full-thickness ear wound. Over 14 days, female mice received daily plasma treatment. Quantitative analysis by transmitted light microscopy demonstrated a significantly accelerated wound re-epithelialization at days 3-9 in comparison with untreated controls. In vitro, cold plasma altered keratinocyte and fibroblast migration, while both cell types showed significant stimulation resulting in accelerated closure of gaps in scratch assays. This plasma effect correlated with the downregulation of the gap junctional protein connexin 43 which is thought to be important in the regulation of wound healing. In addition, plasma induced profound changes in adherence junctions and cytoskeletal dynamics as shown by downregulation of E-cadherin and several integrins as well as actin reorganization. Our results theorize cold plasma to be a beneficial treatment option supplementing existing wound therapies.

show abstract

Section: Resultssupporting

confidence: 87%

A cold plasma jet accelerates wound healing in a murine model of full‐thickness skin wounds

Schmidt

Bekeschus

Wende

et al. 2017

Experimental Dermatology

Self Cite

203

140

View full text Add to dashboard Cite

show abstract

“…The plasma processing of materials including the amine plasma polymerization has gained considerable popularity for the development of bioactive materials, biosensors, antibacterial, and anti‐fungal surfaces . Various processes, monomers, and plasma reactors were employed to obtain the desired properties of the layers and several reviews reported the most prominent results achieved using allylamine, heptylamine, ethylenediamine, NH 3 , NH 3 /C 2 H 4 , and NH 3 /C 2 H 2 plasma polymerizations.…”

Section: Introductionmentioning

confidence: 99%

Cyclopropylamine plasma polymers for increased cell adhesion and growth

Manakhov

Landová

Medalová

et al. 2016

Plasma Processes & Polymers

View full text Add to dashboard Cite

Plasma polymers with NHx groups were deposited from cyclopropylamine in low pressure radio frequency (RF) discharges. The amount of NHx decreased with increasing average power Pav, whereas the layer dissolution in water decreased and high Pav led to a slight film swelling. The C2C12 cells demonstrated very high adhesion to the layers regardless of the deposition conditions. Although the surface chemistry of amine‐rich layers should enhance the cell proliferation, the WST‐1 assay, and immunocytochemistry revealed lower cell proliferation and viability on the layers exhibiting above 18% of relative thickness loss in water. Promising results were obtained on the layers with better water stability and bearing 7–10% of NHx.

show abstract

“…As with the introduction of portable laser technology and associated innovations, the development of mobile devices [244,253] allows multiple local plasma applications. At the moment, these are focused around the therapy of chronic wounds [245,246,247,248,249], tumors [250], and eliminating biofilm on implants [251,252,253]. …”

Section: Future Perspectivesmentioning

confidence: 99%

Consensus on Wound Antisepsis: Update 2018

Krämer

Dissemond²,

Kim³

et al. 2017

Skin Pharmacol Physiol

Self Cite

256

215

View full text Add to dashboard Cite

Wound antisepsis has undergone a renaissance due to the introduction of highly effective wound-compatible antimicrobial agents and the spread of multidrug-resistant organisms (MDROs). However, a strict indication must be set for the application of these agents. An infected or critically colonized wound must be treated antiseptically. In addition, systemic antibiotic therapy is required in case the infection spreads. If applied preventively, the Wounds-at-Risk Score allows an assessment of the risk for infection and thus appropriateness of the indication. The content of this updated consensus recommendation still largely consists of discussing properties of octenidine dihydrochloride (OCT), polihexanide, and iodophores. The evaluations of hypochlorite, taurolidine, and silver ions have been updated. For critically colonized and infected chronic wounds as well as for burns, polihexanide is classified as the active agent of choice. The combination 0.1% OCT/phenoxyethanol (PE) solution is suitable for acute, contaminated, and traumatic wounds, including MRSA-colonized wounds due to its deep action. For chronic wounds, preparations with 0.05% OCT are preferable. For bite, stab/puncture, and gunshot wounds, polyvinylpyrrolidone (PVP)-iodine is the first choice, while polihexanide and hypochlorite are superior to PVP-iodine for the treatment of contaminated acute and chronic wounds. For the decolonization of wounds colonized or infected with MDROs, the combination of OCT/PE is preferred. For peritoneal rinsing or rinsing of other cavities with a lack of drainage potential as well as the risk of central nervous system exposure, hypochlorite is the superior active agent. Silver-sulfadiazine is classified as dispensable, while dyes, organic mercury compounds, and hydrogen peroxide alone are classified as obsolete. As promising prospects, acetic acid, the combination of negative pressure wound therapy with the instillation of antiseptics (NPWTi), and cold atmospheric plasma are also subjects of this assessment.

show abstract

Cold Physical Plasmas in the Field of Hygiene—Relevance, Significance, and Future Applications

Cited by 54 publications

References 295 publications

A cold plasma jet accelerates wound healing in a murine model of full‐thickness skin wounds

A cold plasma jet accelerates wound healing in a murine model of full‐thickness skin wounds

Cyclopropylamine plasma polymers for increased cell adhesion and growth

Consensus on Wound Antisepsis: Update 2018

Contact Info

Product

Resources

About