Cold plasma is well-tolerated and does not disturb skin barrier or reduce skin moisture

Daeschlein, Georg; Scholz, Sebastian; Ahmed, Raees; Majumdar, Abhijit; Woedtke, Thomas von; Haase, Hermann; Niggemeier, Maria; Kindel, E.; Brandenburg, Ronny; Weltmann, K.‐D.; Jünger, Michael

doi:10.1111/j.1610-0387.2012.07857.x

Cited by 68 publications

(51 citation statements)

References 19 publications

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“…Although there is increasing evidence that plasma treatment promotes the healing process of tissue and accelerates wound healing [18]–[21], [55], however the data reported here put forward the idea that argon plasma-treated liquids alter epithelial cell monolayer and impair cell adhesion. These are opposite evidences and should be discussed.…”

Section: Discussionmentioning

confidence: 59%

“…Furthermore, cell physiological processes can be induced by plasma treatment that lead to increased tissue healing [18]–[20] but without disturbing e. g. the skin barrier or moisture [21]. In the healing process of tissues, the adhesion molecules of the cells play a crucial role for the cells' adhesion, migration and proliferation and are therefore, responsible for many cell functions [22]–[24].…”

Section: Introductionmentioning

confidence: 99%

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Persistent Effectivity of Gas Plasma-Treated, Long Time-Stored Liquid on Epithelial Cell Adhesion Capacity and Membrane Morphology

et al. 2014

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Research in plasma medicine includes a major interest in understanding gas plasma-cell interactions. The immediate application of gas plasma in vitro inhibits cell attachment, vitality and cell-cell contacts via the liquid. Interestingly, in our novel experiments described here we found that the liquid-mediated plasma effect is long-lasting after storage up to seven days; i. e. the liquid preserves the characteristics once induced by the argon plasma. Therefore, the complete Dulbecco's Modified Eagle cell culture medium was argon plasma-treated (atmospheric pressure, kINPen09) for 60 s, stored for several days (1, 4 and 7 d) at 37°C and added to a confluent mouse hepatocyte epithelial cell (mHepR1) monolayer. Impaired tight junction architecture as well as shortened microvilli on the cell membrane could be observed, which was accompanied by the loss of cell adhesion capacity. Online-monitoring of vital cells revealed a reduced cell respiration. Our first time-dependent analysis of plasma-treated medium revealed that temperature, hydrogen peroxide production, pH and oxygen content can be excluded as initiators of cell physiological and morphological changes. The here observed persisting biological effects in plasma-treated liquids could open new medical applications in dentistry and orthopaedics.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Persistent Effectivity of Gas Plasma-Treated, Long Time-Stored Liquid on Epithelial Cell Adhesion Capacity and Membrane Morphology

et al. 2014

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“…The most widely established use of plasma is for the sterilization of fragile materials sensitive to thermal damage. Subsequent studies have identified several clinical uses ranging from dental care to various skin diseases such as wound healing, foot fungus, and general hand sterilization without disturbing the skin barrier [26–28]. In contrast to conventional lasers, which are based on thermal-mediated interactions with tissues that result in necrosis and permanent tissue injury, non-thermal plasmas operate at temperatures closer to room temperature to promote focal cell removal without inducing surrounding necrosis.…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Cold Atmospheric Plasma in the Treatment of Malignant Glioma

et al. 2015

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ObjectiveCold atmospheric plasma (CAP) has recently been shown to selectively target cancer cells with minimal effects on normal cells. We systematically assessed the effects of CAP in the treatment of glioblastoma.MethodsThree glioma cell lines, normal astrocytes, and endothelial cell lines were treated with CAP. The effects of CAP were then characterized for viability, cytotoxicity/apoptosis, and cell cycle effects. Statistical significance was determined with student's t-test.ResultsCAP treatment decreases viability of glioma cells in a dose dependent manner, with the ID50 between 90-120 seconds for all glioma cell lines. Treatment with CAP for more than 120 seconds resulted in viability less than 35% at 24-hours posttreatment, with a steady decline to less than 20% at 72-hours. In contrast, the effect of CAP on the viability of NHA and HUVEC was minimal, and importantly not significant at 90 to 120 seconds, with up to 85% of the cells remained viable at 72-hours post-treatment. CAP treatment produces both cytotoxic and apoptotic effects with some variability between cell lines. CAP treatment resulted in a G2/M-phase cell cycle pause in all three cell lines.ConclusionsThis preliminary study determined a multi-focal effect of CAP on glioma cells in vitro, which was not observed in the non-tumor cell lines. The decreased viability depended on the treatment duration and cell line, but overall was explained by the induction of cytotoxicity, apoptosis, and G2/M pause. Future studies will aim at further characterization with more complex pre-clinical models.

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“…Plasma species is one of the key parameters, because it influences the amount of species obtained as well as the energy consumed during the treatment of materials, such as film deposition 7,8 , surface sterilization 9 , polymers surface treatment 9 , dental and skin treatments 10 . This knowledge is of utmost importance not only to obtain better results in respective treatments, but also to offer minimal risk to the operator and user when used in biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Study of the Influence of Variation in Distances Between Electrodes in Spectral DBD Plasma Excitation

Souza¹,

Neto²,

Queiroz³

et al. 2016

Mat. Res.

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Cold plasma is well-tolerated and does not disturb skin barrier or reduce skin moisture

Abstract: All plasma treatments were well-tolerated and did not damage the skin barrier nor cause skin dryness. Cold plasma fulfils basic recommendations for safe use on human skin and as future option may serve as the first physical skin antiseptic.

Cited by 68 publications

References 19 publications

Persistent Effectivity of Gas Plasma-Treated, Long Time-Stored Liquid on Epithelial Cell Adhesion Capacity and Membrane Morphology

Persistent Effectivity of Gas Plasma-Treated, Long Time-Stored Liquid on Epithelial Cell Adhesion Capacity and Membrane Morphology

Differential Effects of Cold Atmospheric Plasma in the Treatment of Malignant Glioma

Study of the Influence of Variation in Distances Between Electrodes in Spectral DBD Plasma Excitation

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