COLD-PRESERVATION OF Lucilia sericata (DIPTERA: CALLIPHORIDAE) PUPAE AND ADULT PRODUCTS AS A NEW VENTURE TO ADULTS REARING

Abstract

Rearing insect colonies in insectarium is often encountered with some problems. Cold-preservation can serve as an alternate option and can save large amount of insects without time-lapse, this method is also found valuable and cost-effective. In present study Lucilia sericata adults were used for cold preservation. One hundred twenty (120) L. sericata pupae were used in this study, these insects were divided in three groups of forty member, and each group was stored in 50 ml tubes containing sawdust and kept at 4°C for 3, 6 &12 months. After the completion of required exposure time (3, 6 and 12 months), pupae were taken out from 4°C and placed under standard maggotarium conditions on sawdust in 40 × 40 cm cages. This was followed by the counting adult number, result of study revealed that after three months, 28 (70%) pupae were transformed into adults. While in case of 6 and 12 months this transformation number was 20 (50%) and 8 (20%) pupae respectively. From the result of study it can be concluded that this method is cost effective and time saving and L. sericata can be reared when ever required. This method opens new horizons for mass rearing of L. sericata for laboratory, research and business requirements * Corresponding author KEYWORDS Cold-preservation Lucilia sericata PupaeMass rearing

The impact of chilling on selected attributes of the blowfly, Calliphoravicina(Robineau-Desvoidy, 1830) (Diptera: Calliphoridae), under laboratory conditions

“…In a previous study, Alipour et al (2018) assessed the effects of long-term cold preservation on the viability of Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) pupae. They found that the percentage of individuals reaching adulthood was decreased with increasing storage time at 4 ºC.…”

Effect of chilling on pupal developmental arrest and subsequent impact on quality control parameters of adult blowfly, Lucilia cuprina (Diptera: Calliphoridae)

“…Adult emergence, pupal weight, adult flight ability, and fecundity decreased with the increase of chilling duration, but storage for a short duration (5 days) at 5 °C was not affected by mentioned parameters [ 16 , 17 ]. The pupae of Lucilia sericata can be stored at 4 °C for one year without uninterrupted rearing [ 18 ]. Queensland fruit fly pupae were highly sensitive to the lowest tested temperatures (13 and 15 °C), when the pupae were stored at 17 °C, pupal development time was more than doubled and only chilling temperatures above 19 °C did not significantly affect the percentage of fliers [ 6 ].Regarding the effect of chilling, though, there are several works on the development of larvae and pupae of Lucilia cuprina at different temperatures and durations [ [19] , [20] , [21] , [22] , [23] ].…”

“…For other species of blowfly, Lucilia sericata [ 24 , 25 ], Phormia regina [ 26 ], the various effects of temperature on life history were observed. Also, the different techniques of preservation were studied for other blowflies [ 9 , 14 , 18 ]. Hence, more studies are needed to get appropriate biological activities of adult flies after the chilling duration of Lucilia cuprina pupae.…”