Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana

Bittner, Andras; Buer, Jörn van; Baier, Margarete

doi:10.21203/rs.2.19977/v1

Cited by 2 publications

(2 citation statements)

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“…It may be hypothesized that the ability to respond to priming treatment might be genetically controlled but, to the best of our knowledge, no data are available concerning this important aspect. Thus, further progresses are needed not only to identify the set of genes that are regulated by priming, but also the set of genes that putatively regulate priming response and efficiency themselves (Bittner et al, 2020). Another challenge is to identify appropriate treatments able to restore the vigor of old dry seed lots in order to increase their mean percentage of germination to values compatible with commercial purposes (Miladinov et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Priming seeds of sorghum and sudangrass using water and aquatic extracts of willow and banana

Mamlić¹,

Đukić²,

Dozet³

et al. 2022

Acta agriculturae Serbica

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The aim of this study was to examine the impact of seed priming on seed quality parameters (germination energy, germination, seedling length and vigor index) of sorghum (Sorghum bicolor L. Moench) and sudangrass (Sorghum sudanense Pers.). The experiment was performed at the Institute of Field and Vegetable Crops, Novi Sad, Serbia. The research was conducted on the seeds of the sorghum variety NS Džin and sudangrass variety Srem. Distilled water and aqueous extracts of young willow branches (Salix matsudana) and mature banana fruits (Musa x paradisiaca) were used to prime the seeds. The seeds were primed for: 1 h, 3 h, 6 h, 12 h and 24 h. The results showed that priming sorghum and sudangrass seeds in aqueous extracts of young willow branches and mature banana fruits can have a positive effect on seed quality parameters: germination energy, germination, seedling length and vigor index. The greatest effect on germination energy and seed germination was achieved in sudangrass by using an aqueous extract of young willow branches. The increase was 9.30% and 9.20%, respectively. Priming sorghum seeds in aquatic extracts of mature banana fruits had the greatest effect on seedling length and vigor index. The increase was 36.86% and 40.33%, respectively. For all research parameters, priming for 3 h was the most effective. However, in addition to the positive effect, priming can also have a negative effect. The most significant reduction was found on sorghum seeds, when primed in an aqueous extract of mature banana fruit for 24 hours. Germination energy and germination were reduced by 7.14% and 9.30%, respectively.

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Section: Introductionmentioning

confidence: 99%

Priming seeds of sorghum and sudangrass using water and aquatic extracts of willow and banana

Mamlić¹,

Đukić²,

Dozet³

et al. 2022

Acta agriculturae Serbica

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“…Although well studied in vernalization, little is known about the function of TM in cold acclimation and morphological responses to cold. Recent work in Arabidopsis has demonstrated the existence of cold‐stress memories affecting freezing tolerance, and transcriptomic and metabolomic responses to chilling, and has highlighted the involvement of chromatin modifications in cold memory (Zuther et al ., 2019; Vyse et al ., 2020), though other studies have also described cold memory and changes to chromatin states associated with cold exposure other than vernalization (Zhu et al ., 2008; Kwon et al ., 2009; Mayer et al ., 2015; Bittner et al ., 2020). Unlike in Arabidopsis, however, vernalization and the expression of freezing tolerance encompassing cold acclimation and morphology are interconnected in B. distachyon , notably through VRN1 which, other than regulating vernalization, influences cold acclimation and the acquisition of a winter‐hardy morphology (Bond et al ., 2011; Mayer et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional memories mediate the plasticity of cold stress responses to enable morphological acclimation in Brachypodium distachyon

Mayer

Charron

2020

New Phytologist

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Summary Plants that successfully acclimate to stress can resume growth under stressful conditions. The grass Brachypodium distachyon can grow a cold‐adaptive morphology during cold acclimation. Studies on transcriptional memory (TM) have revealed that plants can be primed for stress by adjusting their transcriptional responses, but the function of TM in stress acclimation is not well understood. We investigated the function of TM during cold acclimation in B. distachyon. Quantitative polymerase chain reaction (qPCR), RNA‐seq and chromatin immunoprecipitation qPCR analyses were performed on plants exposed to repeated episodes of cold to characterize the presence and stability of TM during the stress and growth responses of cold acclimation. Transcriptional memory mainly dampened stress responses as growth resumed and as B. distachyon became habituated to cold stress. Although permanent on vernalization gene VRN1, TMs were short‐term and reversible on cold‐stress genes. Growing under cold conditions also coincided with the acquisition of new and targeted cold‐induced transcriptional responses. Overall, TM provided plasticity to cold stress responses during cold acclimation in B. distachyon, leading to stress habituation, acquired stress responses, and resumed growth. Our study shows that chromatin‐associated TMs are involved in tuning plant responses to environmental change and, as such, regulate both stress and developmental components that characterize cold‐climate adaptation in B. distachyon.

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Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana

Cited by 2 publications

References 70 publications

Priming seeds of sorghum and sudangrass using water and aquatic extracts of willow and banana

Priming seeds of sorghum and sudangrass using water and aquatic extracts of willow and banana

Transcriptional memories mediate the plasticity of cold stress responses to enable morphological acclimation in Brachypodium distachyon

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