Cold-regulated protein (SlCOR413IM1) confers chilling stress tolerance in tomato plants

Ma, Xiaocui; Chen, Chong; Yang, Minmin; Dong, Xinchun; Lv, Wei; Meng, Qingwei

doi:10.1016/j.plaphy.2018.01.003

Cited by 48 publications

(32 citation statements)

References 58 publications

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“…Based on these findings, the present paper reveals a linear regressive relationship between freezing sensitivity index (H) and Fv/Fm ratio of frozen group to control group (H Fv/Fm ): H = 60.31 − 50.09 R Fv/Fm ( p < 0.01) (Table S1). Chloroplast is extremely sensitive to low temperature stress and also one of the first organelles affected by suboptimal growing temperature [11]. Low temperature stress induced thylakoid super-complex rearrangements, resulting in differentiation of chloroplast structure between cold sensitive and cold tolerant plants [15].…”

Section: Discussionmentioning

confidence: 99%

“…Various ecotypes of crops showed distinct differences in their potential to acclimate to cold and hot stress [9]. Chloroplast, a photosynthesis site in a plant, is one of the first organelles affected by cold stress and it extremely sensitive to cold stress [10,11]. Chloroplast proteolytic machinery is involved in repairing damage incurred to the photosynthetic machinery upon exposure to cold stress [12].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Freezing on Photosystem II and Assessment of Freezing Tolerance of Tea Cultivar

Shi

Cai

et al. 2019

Plants

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Freezing tolerant tea cultivars are urgently needed. The tea cultivars with highly freezing tolerance showed resistance to freezing stress induced photoinhibition. Freezing sensitivity index (H) of 47 tea clonal cultivars was investigated after severe freezing winter in 2016. To develop instrumental methods for freezing tolerance selection, the maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm) and leaf color indicator a on the Hunter color scale were determined on control group (non-frozen) and frozen group (being frozen at −15 °C for 2 h and then stood at 20 °C for 5 h) of the cultivars. When the two indicators were expressed as the ratios (RFv/Fm and Ra) of frozen group to control group, linear regression of the freezing sensitivity index (H) upon the RFv/Fm and Ra produced significant relationship respectively, i.e., H = 60.31 − 50.09 RFv/Fm (p < 0.01) and H = 30.03 − 10.82 Ra (p < 0.01). Expression of gene psbA encoding D1 protein and gene psbD encoding D2 protein in PSII showed that the frezzing tolerant tea cultivars maintained a high expression level of psbA after freezing stress, which is considered to be beneficial to de novo synthesis of D1 protein and sustaining PSII activity. These findings can provide instrumental tools for assessing freezing tolerance of tea cultivars in tea breeding program.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Freezing on Photosystem II and Assessment of Freezing Tolerance of Tea Cultivar

Shi

Cai

et al. 2019

Plants

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“…Twenty-four hours after heat treatment, leaves from WT and slmapk3 mutants were used for trypan blue staining analysis [43]. Detached leaves were soaked in 0.4% trypan blue solution at room temperature for 8 h. The photo was taken after decolorizing in boiling fixing liquid (lactic acid: glycerol: ethanol = 1:1:4).…”

Section: Methodsmentioning

confidence: 99%

Knockout of SlMAPK3 enhances tolerance to heat stress involving ROS homeostasis in tomato plants

et al. 2019

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Background High temperature is a major environmental stress that limits plant growth and agriculture productivity. Mitogen-activated protein kinases (MAPKs) are highly conserved serine and threonine protein kinases that participate in response to diverse environmental stresses in plants. A total of 16 putative SlMAPK genes are identified in tomato, and SlMAPK3 is one of the most extensively studied SlMAPKs . However, the role of SlMAPK3 in response to heat stress is not clearly understood in tomato plants. In this study, we performed functional analysis of SlMAPK3 for its possible role in response to heat stress. Results qRT-PCR analyses revealed that SlMAPK3 relative expression was depressed by heat stress. Here, wild-type (WT) tomato plants and CRISPR/Cas9-mediated slmapk3 mutant lines (L8 and L13) were used to investigate the function of SlMAPK3 in response to heat stress. Compared with WT plants, slmapk3 mutants exhibited less severe wilting and less membrane damage, showed lower reactive oxygen species (ROS) contents, and presented higher both activities and transcript levels of antioxidant enzymes, as well as elevated expressions of genes encoding heat stress transcription factors ( HSFs ) and heat shock proteins ( HSPs ). Conclusions CRISPR/Cas9-mediated slmapk3 mutants exhibited more tolerance to heat stress than WT plants, suggesting that SlMAPK3 was a negative regulator of thermotolerance. Moreover, antioxidant enzymes and HSPs / HSFs genes expression were involved in SlMAPK3 -mediated heat stress response in tomato plants. Electronic supplementary material The online version of this article (10.1186/s12870-019-1939-z) contains supplementary material, which is available to authorized users.

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“…Low temperatures are one of the major environmental stresses that limit growth, development, productivity and geographical distribution of plants (Ahanger et al 2017;Zhao et al 2018). Based on the intensity of low temperatures, induced stress can be classi ed as cold (temperatures within the range 0-20°C) or freezing stress (temperatures below 0°C) (Ma et al 2018). Low temperatures trigger numerous processes in plants at molecular, biochemical and morphological level, which may be represented as alert, acclimation, tolerance, exhaustion (under prolonged and intense stresses) and/or recovery phase (after stress factor removal; may lead to a new homeostasis maintenance) (Kosová et al 2015).…”

Section: Introductionmentioning

confidence: 99%

A Study of Arabidopsis Cold Stress Tolerance Improvement via AthHOS1-Targeting HOS1-AmiRNA Approach

Karimi

Shiran

Rabei

et al. 2021

Preprint

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In this study the artificial microRNAs (amiRNAs) technology targeting HOS1 gene was tested for its applicability for the improvement of cold stress tolerance in Landsberg-0 (Ler-0) ecotype of Arabidopsis thaliana. The chosen approach was designed to suppress AtHOS1 gene expression through the overexpression of amiRNA-HOS1. The effect of AtHOS1-amiRNA overexpression to transgenic plants’ response to cold stress was determined by Real Time PCR. The expression levels of amiRNA and its target, AtHOS1 gene, were observed in 3-week old seedlings of T3 generation and in wild-type plants after 6h, 12h, 24h, 48h and 96h of their exposure to cold stress (4ºC). Comparative analysis revealed that AtHOS1-amiRNA negatively regulated AtHOS1 in transgenic plants upon plants lengthen exposure (for 48h and 96h) to low temperature (Pearson’s correlation coefficient of -0.407; P < 0.05). Even though prolonged cold stress caused extended up regulation of AtHOS1 in wild type plants, in transgenic plants AtHOS1-amiRNA suppression disturbed expected AtHOS1 circadian rhythm by preventing further AtHOS1 up regulation. Moreover, transgenic plants showed AtHOS1 down regulation 96h after the cold stress onset, due to sufficient overexpression of AtHOS1-amiRNA, which allowed cold signaling amplification in transgenic plants. As a result of that, cold-acclimated transformed plants displayed 17% higher freezing tolerance (-1°C to -8°C) in comparison to wild type plants, demonstrating the success of chosen approach in improving Arabidopsis tolerance to low temperatures, at least in Ler-0 ecotype.

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Cold-regulated protein (SlCOR413IM1) confers chilling stress tolerance in tomato plants

Cited by 48 publications

References 58 publications

Effect of Freezing on Photosystem II and Assessment of Freezing Tolerance of Tea Cultivar

Effect of Freezing on Photosystem II and Assessment of Freezing Tolerance of Tea Cultivar

Knockout of SlMAPK3 enhances tolerance to heat stress involving ROS homeostasis in tomato plants

A Study of Arabidopsis Cold Stress Tolerance Improvement via AthHOS1-Targeting HOS1-AmiRNA Approach

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