Cold-Responsive Regulation of a Flower-Preferential Class III Peroxidase Gene, OsPOX1, in Rice (Oryza sativa L.)

Kim, Sung-Hyun; Choi, Hyung-Sub; Cho, Young‐Chan; Kim, Seong-Ryong

doi:10.1007/s12374-011-9194-3

Cited by 24 publications

(16 citation statements)

References 35 publications

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“…The genes related to POD in rice, such as OsAPX1 and OsAPX2, were induced after rice plants were exposed to low temperatures (Caverzan et al 2012). The transgenic rice plants overexpressing a cytosolic Ascorbate peroxidase1 (APX1) gene (OsAPXa) which exhibited higher APX activity in spikelets than in WT plants, sustained higher levels of APX activity under cold stress, resulting in enhanced cold tolerance at the booting stage (Sato et al 2011;Kim et al 2012). The mutations in catalase3 (OsCAT3), in rice, resulted in an increase of H 2 O 2 in the leaves, which consequently promoted Nitric oxide (NO) production via the activation of nitrate reductase.…”

Section: Discussionmentioning

confidence: 99%

Knockout of OsPRP1, a gene encoding proline-rich protein, confers enhanced cold sensitivity in rice (Oryza sativa L.) at the seedling stage

et al. 2019

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Proline-rich proteins (PRPs) play multiple physiological and biochemical roles in plant growth and stress response. In this study, we reported that the knockout of OsPRP1 induced cold sensitivity in rice. Mutant plants were generated by CRISPR/ Cas9 technology to investigate the role of OsPRP1 in cold stress and 26 mutant plants were obtained in T 0 generation with the mutation rate of 85% including 15% bi-allelic, 53.3% homozygous, and 16.7% heterozygous and 16 T-DNA-free lines in T 1 generation. The conserved amino acid sequence was changed and the expression level of OsPRP1 was reduced in mutant plants. The OsPRP1 mutant plants displayed more sensitivity to cold stress and showed low survival rate with decreased root biomass than wild-type (WT) and homozygous mutant line with large fragment deletion was more sensitive to low temperature. Mutant lines accumulated less antioxidant enzyme activity and lower levels of proline, chlorophyll, abscisic acid (ABA), and ascorbic acid (AsA) content relative to WT under low-temperature stress. The changes of antioxidant enzymes were examined in the leaves and roots with exogenous salicylic acid (SA) treatment which resulted in increased activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) under cold stress, while enzyme antioxidant activity was lower in untreated seedlings which showed that exogenous SA pretreatment could alleviate the low-temperature stress in rice. Furthermore, the expression of three genes encoding antioxidant enzyme activities (SOD4, POX1, and OsCAT3) was significantly down-regulated in the mutant lines as compared to WT. These results suggested that OsPRP1 enhances cold tolerance by modulating antioxidants and maintaining cross talk through signaling pathways. Therefore, OsPRP1 gene could be exploited for improving cold tolerance in rice and CRISPR/Cas9 technology is helpful to study the function of a gene by analyzing the phenotypes of knockout mutants generated.

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Section: Discussionmentioning

confidence: 99%

Knockout of OsPRP1, a gene encoding proline-rich protein, confers enhanced cold sensitivity in rice (Oryza sativa L.) at the seedling stage

et al. 2019

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“…Of note, a set of the DEGs associated with cell wall and senescence were significantly altered in del1 plants, such as the PME gene (OsPME1), b-expansin gene (OsEXPB2), peroxidase precursor gene (OsPOX1), protochlorophyllide oxidoreductase B gene (OsPORA), etc. (Kim et al, 2012;Sakuraba et al, 2013;Zou et al, 2015;Fang et al, 2016;Supplemental Table S4). These results suggested that the mutation of DEL1 might affect the expression of cell wall-and senescence-related genes in an indirect manner.…”

Section: Activation Of Cell Wall-and Senescence-related Genes Revealementioning

confidence: 99%

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

et al. 2017

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To better understand the molecular mechanisms behind plant growth and leaf senescence in monocot plants, we identified a mutant exhibiting dwarfism and an early-senescence leaf phenotype, termed dwarf and early-senescence leaf1 (del1). Histological analysis showed that the abnormal growth was caused by a reduction in cell number. Further investigation revealed that the decline in cell number in del1 was affected by the cell cycle. Physiological analysis, transmission electron microscopy, and TUNEL assays showed that leaf senescence was triggered by the accumulation of reactive oxygen species. The DEL1 gene was cloned using a map-based approach. It was shown to encode a pectate lyase (PEL) precursor that contains a PelC domain. DEL1 contains all the conserved residues of PEL and has strong similarity with plant PelC. DEL1 is expressed in all tissues but predominantly in elongating tissues. Functional analysis revealed that mutation of DEL1 decreased the total PEL enzymatic activity, increased the degree of methylesterified homogalacturonan, and altered the cell wall composition and structure. In addition, transcriptome assay revealed that a set of cell wall function-and senescence-related gene expression was altered in del1 plants. Our research indicates that DEL1 is involved in both the maintenance of normal cell division and the induction of leaf senescence. These findings reveal a new molecular mechanism for plant growth and leaf senescence mediated by PECTATE LYASE-LIKE genes.

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“…The higher activities of antioxidant enzymes often expressed in transgenic rice lines can indicate their relatively improved cold tolerance compared to wild-type plants (Bonnecarrère et al [ 2011 ]; Huang et al [ 2009 ]). Increased activities of antioxidant enzyme, including OsPOX1, APXa, and related kinase OsTrx23 (Kim et al [ 2011 ]; Sato et al [ 2011 ]; Xie et al [ 2012 ]) may serve to maintain the functions of many other genes during cold stress. Therefore, rather than only physical evaluation of whole-plant cold tolerance, evaluation of antioxidant enzyme activities could both reveal cold-tolerant plants and reveal the mechanisms of cold tolerance.…”

Section: Reviewmentioning

confidence: 99%

Rice and cold stress: methods for its evaluation and summary of cold tolerance-related quantitative trait loci

Zhāng

Chen

Wang

et al. 2014

Rice

228

196

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Cold stress adversely affects rice (Oryza sativa L.) growth and productivity, and has so far determined its geographical distribution. Dissecting cold stress-mediated physiological changes and understanding their genetic causes will facilitate the breeding of rice for cold tolerance. Here, we review recent progress in research on cold stress-mediated physiological traits and metabolites, and indicate their roles in the cold-response network and cold-tolerance evaluation. We also discuss criteria for evaluating cold tolerance and evaluate the scope and shortcomings of each application. Moreover, we summarize research on quantitative trait loci (QTL) related to cold stress at the germination, seedling, and reproductive stages that should provide useful information to accelerate progress in breeding cold-tolerant rice.

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Cold-Responsive Regulation of a Flower-Preferential Class III Peroxidase Gene, OsPOX1, in Rice (Oryza sativa L.)

Cited by 24 publications

References 35 publications

Knockout of OsPRP1, a gene encoding proline-rich protein, confers enhanced cold sensitivity in rice (Oryza sativa L.) at the seedling stage

Knockout of OsPRP1, a gene encoding proline-rich protein, confers enhanced cold sensitivity in rice (Oryza sativa L.) at the seedling stage

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

Rice and cold stress: methods for its evaluation and summary of cold tolerance-related quantitative trait loci

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