Cold-restraint stress reduces [3H]etorphine binding to rat brain membranes: Influence of acute and chronic morphine and naloxone

Hnatowich, Mark; Labella, F. S.; Kiernan, Kathleen M.; Glavin, Gary B.

doi:10.1016/0006-8993(86)91434-4

Cited by 21 publications

(4 citation statements)

References 26 publications

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“…Rats were tested for both mechanical and thermal sensitivities over time during the course of the studies. To minimize the stress of handling during the testing procedures, rats were handled daily for 20 minutes during the week prior to testing [15,16]. On the first day of behavioral testing, the rats were acclimated to their surroundings for 1 hour.…”

Section: Methodsmentioning

confidence: 99%

Insulin Is Essential for the Recovery from Allodynia Induced by Complete Freund's Adjuvant

Casey¹,

Paúl²,

Gould³

2010

Pain Med

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Section: Methodsmentioning

confidence: 99%

Insulin Is Essential for the Recovery from Allodynia Induced by Complete Freund's Adjuvant

Casey¹,

Paúl²,

Gould³

2010

Pain Med

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“…Stukey et al (23) showed lower density of the l opioid receptor subtype in brain homogenates of rats submitted to inescapable shock compared to those submitted to escapable shock, without changes in the affinity (Kd). A reduction in the number of opioid receptors after exposure to a 3-h restraint period in the cold was also observed (34). In these early studies, the alterations in opioid binding were observed shortly after exposure to acute stress.…”

Section: Discussionmentioning

confidence: 90%

Repeated Restraint Stress Reduces Opioid Receptor Binding in Different Rat CNS Structures

et al. 2005

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Different effects of exposure to acute or to repeated stress have been observed upon the nociceptive response in rats. In the present study, we repeatedly submitted Wistar rats to restraint for 40 days, a treatment known to induce an increase in the nociceptive response in the tail-flick test. Afterwards, the effect of repeated restraint stress on the density of opioid receptors in rat spinal cord, frontal cortex, and hippocampus was investigated. Results showed that repeatedly stressed rats displayed a significant decrease in opioid receptors density in all structures studied; cortex (141.3 +/- 5.7 for control and 103.3 +/- 15.9 for stressed rats), hippocampus (92.4 +/- 7.2 for control and 64.8 +/- 7.7 for stressed rats), and spinal cord (122.2 +/- 12.8 for control and 79.7 +/- 9.7 for stressed rats). These findings suggest opioid mediation of the altered responses observed in these repeatedly-stressed animals, although the participation of non-opioid mechanisms in this phenomenon cannot be ruled out.

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“…A role of increased β‐endorphin in µ opioid receptor downregulation is supported by other studies in which β‐endorphin levels were increased in vivo due to exposure to a stressor. For example, decreases in binding to µ opioid receptors have been reported following inescapable foot shock (43), cold‐restraint stress (44) and mild handling stress (45). These stressors result in elevated β‐endorphin levels and the observed changes in opioid receptor binding have been attributed to the increase in β‐endorphin.…”

Section: Discussionmentioning

confidence: 99%

Ablation of Pituitary Pro‐Opiomelanocortin (POMC) Cells Produces Alterations in Hypothalamic POMC mRNA Levels and Midbrain µ Opioid Receptor Binding in a Conditional Transgenic Mouse Model

Zhou

Unterwald

et al. 2001

J Neuroendocrinology

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The hypothalamic-pituitary-adrenal (HPA) axis is regulated by stress-related excitatory inputs, and various inhibitory and negative-feedback controls by glucocorticoids and opioids, including pro-opiomelanocortin (POMC)-derived peptides. The role of POMC-derived peptides of pituitary origin in the modulation of brain POMC mRNA expression and opioid receptor binding was investigated using a line of transgenic mice that express a fusion gene composed of the pituitary expression-specific promoter region of the POMC gene driving the herpes simplex viral-1 thymidine kinase (TK). Male adult mice were treated with the antiherpes agent ganciclovir that selectively ablates cells expressing TK. Following treatment, POMC mRNA levels, measured by quantitative solution hybridization/RNase protection assays, were decreased by 48% in the pituitary of the TK+/+ mice, reflecting an expected loss of the pituitary corticotrope POMC cells. This treatment also significantly lowered pituitary beta-endorphin immunoreactivity content and plasma concentrations of corticosterone. In contrast, POMC mRNA levels were increased by 79% in the hypothalamus of the TK+/+ mice with pituitary POMC cell ablation. Binding of [(3)H]DAMGO to mu opioid receptors, as measured by quantitative autoradiography, was significantly reduced in several brain regions including the central grey, median raphe and superficial grey layer of the superior colliculus. These regions are innervated by hypothalamic POMC neurones. No significant differences in binding to either kappa or delta opioid receptors were found in the brain regions studied. These results suggest that POMC-derived peptides of pituitary origin may exert a tonic negative-feedback effect on hypothalamic POMC neurones. In turn, the downregulation of central mu opioid receptors in this model may be mediated through a mechanism related to hypothalamic POMC overexpression.

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Cold-restraint stress reduces [3H]etorphine binding to rat brain membranes: Influence of acute and chronic morphine and naloxone

Cited by 21 publications

References 26 publications

Insulin Is Essential for the Recovery from Allodynia Induced by Complete Freund's Adjuvant

Insulin Is Essential for the Recovery from Allodynia Induced by Complete Freund's Adjuvant

Repeated Restraint Stress Reduces Opioid Receptor Binding in Different Rat CNS Structures

Ablation of Pituitary Pro‐Opiomelanocortin (POMC) Cells Produces Alterations in Hypothalamic POMC mRNA Levels and Midbrain µ Opioid Receptor Binding in a Conditional Transgenic Mouse Model

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