Cold-sensing regulates Drosophila growth through insulin-producing cells

Li, Qiaoran; Gong, Zhefeng

doi:10.1038/ncomms10083

Cited by 64 publications

(51 citation statements)

References 45 publications

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“…; Puig and Tijan ; Giannakou et al. ; Teleman ; Puig and Mattila ; Li and Gong ; Zhang et al. ), we phenotyped replicated population cage cultures of the alternative alleles at two temperatures (18°C, 25°C) and on two commonly used diets that differ mainly in their sugar source (sucrose vs. molasses) and content.…”

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confidence: 99%

A clinal polymorphism in the insulin signaling transcription factorfoxocontributes to life‐history adaptation inDrosophila*

et al. 2019

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A fundamental aim of adaptation genomics is to identify polymorphisms that underpin variation in fitness traits. In Drosophila melanogaster, latitudinal life‐history clines exist on multiple continents and make an excellent system for dissecting the genetics of adaptation. We have previously identified numerous clinal single‐nucleotide polymorphism in insulin/insulin‐like growth factor signaling (IIS), a pathway known from mutant studies to affect life history. However, the effects of natural variants in this pathway remain poorly understood. Here we investigate how two clinal alternative alleles at foxo, a transcriptional effector of IIS, affect fitness components (viability, size, starvation resistance, fat content). We assessed this polymorphism from the North American cline by reconstituting outbred populations, fixed for either the low‐ or high‐latitude allele, from inbred DGRP lines. Because diet and temperature modulate IIS, we phenotyped alleles across two temperatures (18°C, 25°C) and two diets differing in sugar source and content. Consistent with clinal expectations, the high‐latitude allele conferred larger body size and reduced wing loading. Alleles also differed in starvation resistance and expression of insulin‐like receptor, a transcriptional target of FOXO. Allelic reaction norms were mostly parallel, with few GxE interactions. Together, our results suggest that variation in IIS makes a major contribution to clinal life‐history adaptation.

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confidence: 99%

A clinal polymorphism in the insulin signaling transcription factorfoxocontributes to life‐history adaptation inDrosophila*

et al. 2019

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“…Here we characterize the effects of these clinal foxo genotypes on several fitness-related traits (egg-to-adult survival, proxies of size, starvation resistance, and fat content) by measuring phenotypes on replicate populations of the two alternative alleles under different environmental assay conditions in the laboratory. Because temperature gradients are thought to underpin-at least partly-latitudinal clines (e.g., de Jong and Bochdanovits 2003;Kapun et al 2016b; and references therein), and because both diet and temperature modulate IIS (e.g., Britton et al 2002;Kramer et al 2003;Puig and Tijan 2005;Giannakou et al 2008;Teleman 2010;Puig and Mattila 2011;Li and Gong 2015;Zhang et al 2015), we phenotyped replicated population cage cultures of the alternative alleles at two temperatures (18°C, 25°C) and on two commonly used diets that differ mainly in their sugar source (sucrose vs. molasses) and content.…”

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confidence: 99%

A clinal polymorphism in the insulin signaling transcription factorfoxocontributes to life-history adaptation inDrosophila

Durmaz

Rajpurohit

Betancourt

et al. 2018

Preprint

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A fundamental aim of adaptation genomics is to identify polymorphisms that underpin variation in fitness traits. In Drosophila melanogaster, latitudinal life-history clines exist on multiple continents and make an excellent system for dissecting the genetics of adaptation. We have previously identified numerous clinal single-nucleotide polymorphism in insulin/insulin-like growth factor signaling (IIS), a pathway known from mutant studies to affect life history. However, the effects of natural variants in this pathway remain poorly understood. Here we investigate how two clinal alternative alleles at foxo, a transcriptional effector of IIS, affect fitness components (viability, size, starvation resistance, fat content). We assessed this polymorphism from the North American cline by reconstituting outbred populations, fixed for either the low-or high-latitude allele, from inbred DGRP lines. Because diet and temperature modulate IIS, we phenotyped alleles across two temperatures (18°C, 25°C) and two diets differing in sugar source and content. Consistent with clinal expectations, the high-latitude allele conferred larger body size and reduced wing loading. Alleles also differed in starvation resistance and expression of insulin-like receptor, a transcriptional target of FOXO. Allelic reaction norms were mostly parallel, with few GxE interactions. Together, our results suggest that variation in IIS makes a major contribution to clinal life-history adaptation. K E Y W O R D S : Adaptation, cline, insulin signaling, life history, plasticity, pleiotropy.

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“…However, whether the effect is caused by a mere altered chemical reaction norm or whether temperature is actively sensed and developmental programs are adjusted accordingly, is still under debate. There are evidences for both scenarios 13,14 and they might not exclude each other.…”

Section: Introductionmentioning

confidence: 91%

Bacteria- and temperature-regulated peptides modulate beta-catenin signaling inHydra

Taubenheim

Willoweit-Ohl

Knop

et al. 2019

Preprint

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Animal development has traditionally been viewed as an autonomous process directed by the host genome. But in many animals biotic and abiotic cues, like temperature and bacterial colonizers, provide signals for multiple developmental steps. Hydra offers unique features to encode these complex interactions of developmental processes with biotic and abiotic factors. Here, we used the model animal Hydra to investigate the impact of bacterial colonizers and temperature on the pattern formation process. In Hydra, formation of the head organizer involves the canonical Wnt pathway. Treatment with alsterpaullone (ALP) results in acquiring characteristics of the head organizer in the body column. Intriguingly, germ-free Hydra polyps are significantly more sensitive to ALP compared to control polyps. In addition to microbes, β -catenin dependent pattern formation is also affected by temperature. Gene expression analyses led to the identification of two small secreted peptides, named Eco1 and Eco2, being upregulated in the response to both, Curvibacter sp, the main bacterial colonizer of Hydra, and low temperatures. Loss-of function experiments revealed that Eco peptides are involved in the regulation of pattern formation and have an antagonistic function to Wnt signaling in Hydra. Our results in an early branching metazoan suggest that the Wnt pathway evolved as a signaling hub integrating environmental signals, like bacterial signals and temperature, into developmental processes and stem cell behavior.

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Cold-sensing regulates Drosophila growth through insulin-producing cells

Cited by 64 publications

References 45 publications

A clinal polymorphism in the insulin signaling transcription factorfoxocontributes to life‐history adaptation inDrosophila*

A clinal polymorphism in the insulin signaling transcription factorfoxocontributes to life‐history adaptation inDrosophila*

A clinal polymorphism in the insulin signaling transcription factorfoxocontributes to life-history adaptation inDrosophila

Bacteria- and temperature-regulated peptides modulate beta-catenin signaling inHydra

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