Cold storage of in vitro cultures of hybrid poplar shoots (Populus alba L. x P. grandidentata Michx.)

Abstract

Shoot cultures of P. alba x P. grandidentata 'Crandon' were maintained for more than 5 years at 4°C with minimal growth. The highest survival after 2 years and 5 years of cold storage were 70% and 25% respectively using 1-month of pre-storage culture on MS medium containing 1.33/zMBA. When 5-year-old cold-stored shoot cultures were transferred to the greenhouse, color variations were observed. The frequencies of albino and red pigmented plants were 0.25% and 12.8%, respectively. A rosette type growth pattern was also observed on 0.3% of the long-term cold-stored plants. During long-term cold storage there were local disruption of cambium connections and the accumulations of chemicals in some cells, as observed by light microscopy.

Insertional mutagenesis in Populus: relevance and feasibility

“…Cold storage under low temperature and nutrient deficiency. Although this method does not require field maintenance and is relatively more efficient than continued subculture in normal in vitro programs (Son et al 1991), it is suitable mainly for short-term storage (e.g., 1 to 3 years). .…”

Somatic Embryogenesis and Synthetic Seed I

“…Mass propagation via a tissue culture system has been extensively studied in Populus species (Ahuja 1987;Son and Hall 1990a,b). To obtain rapid true-to-type micropropagules, the axillary branching method has been most commonly used (Whitehead and Giles 1977;Chun et al 1986;Son et al 1991). Even though a large-scale propagation system was successfully demonstrated in some genotypes for commercial purposes there are still limitations, such as lack of reliable systems for increasing the rate of multiplication, for simplifying procedures, and for easy handling.…”

Efficient Technique for Long-Term <i>in Vitro</i> Storage of Transgenic Aspen Genotypes

“…It is the simplest way to limit the growth of plant material in vitro. Culture growth can be slowed down in several ways, including by keeping the culture at low temperatures [6] [7] in the presence of growth regulators such as abscisic acid (ABA), by reducing sucrose levels or adding osmotically active substances (e.g., 3% mannitol) to the culture medium [8] [9]. Exposure to low temperatures results in increased accumulation of unsaturated lipids on cell membranes and hence their thickening and slowing down of cell division and elongation.…”