Cold storage of the sperm of the endemic trout Oncorhynchus mykiss nelsoni: a strategy for short-term germplasm conservation of endemic species

Aguilar-Juárez, Marisela; Ruíz-Campos, Gorgonio; Paniagua-Chávez, Carmen G.

doi:10.7550/rmb.36352

Cited by 16 publications

(10 citation statements)

References 50 publications

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“…Our results demonstrated that sperm functions are affected significantly by the age of the broodstock males and the time of cold storage, decreasing the sperm motility, viability, MMP, and increasing DNA frag and

O_{2}^{-}

level. These results are consistent with those obtained in previous investigations showing that short‐term storage of fish semen at low temperatures induces a significantly decrease of sperm functions, impairing motility, viability (Aguilar‐Juárez et al., ; Aramli et al., ; Şahin et al., ; Shaliutina, , ; Trigo et al., ), MMP (Trigo et al., ), increasing DNA frag (Pérez‐Cerezales et al., ; Shaliutina, , ;) and OS indices (Aramli et al., ; Shaliutina, , ). In the present study, our results of

O_{2}^{-}

level during cold storage provide new information in agreement with those reported by Shaliutina et al.…”

Section: Discussionsupporting

confidence: 91%

“…Short‐term storage is the most common technique utilised in the Chilean salmon industry, and previous researches have shown that it induces a significant decrease in sperm function (Aguilar‐Juárez, Ruiz‐Campos, & Paniagua‐Chávez, ; Aramli, Kalbassi, Nazari, & Aramli, ; Hajirezaee et al., ; Pérez‐Cerezales et al., ; Şahin, Zeki, & Balta, ; Shaliutina, , ; Trigo et al., ). It is consensually recognised that only spermatozoa of good quality with concentration ≥12 × 10 9 spermatozoa/ml, spermatocrit between 10% and 20%, and motility, viability, MMP >80% and DNA frag < 10% (Figueroa et al., , ; Merino et al., ; Pérez‐Cerezales, Gutiérrez‐Adán, Martínez‐Páramo, Beirãoa, & Herráez, ; Trigo et al., ) can be used for assisted reproductive techniques (ART).…”

Section: Introductionmentioning

confidence: 99%

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Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)

et al. 2017

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The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus mykiss) in different reproductive ages (2, 3 and 4 years old). Sperm samples of each reproductive age were stored in Storfish during 10 days at 4°C, and then, motility, viability, mitochondrial function (MMP), superoxide anion (O2-) level and DNA fragmentation (DNA ) were assessed. The results demonstrated that sperm function parameters were affected significantly by the age of the males and the time of storage. Motility, viability and MMP significantly decreased, and DNA and O2- level increased with the age increment and the time of storage. In conclusion, sperm quality of 2 and 3 years old were superior to those of 4 years old, based on higher quality of various sperm functions such as motility, viability, MMP, DNA integrity and level O2- during short-term storage. This information must be considered for optimum utilization of broodstock males in aquaculture.

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O_{2}^{-}

O_{2}^{-}

level during cold storage provide new information in agreement with those reported by Shaliutina et al.…”

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)

et al. 2017

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“…Free amino acids like methionine improve spermatozoa viability in rainbow trout (Lahnsteiner, ). Moreover, dilution ratios of 1:2, 1:3, 1:6, 1:9 and 1:50 have been usually used in previously studies, and the higher dilution rates have been found preferable for rainbow trout spermatozoa in parallel with the current study (Aguilar‐Juárez, Ruiz‐Campos, & Paniagua‐Chávez, ; He & Woods, ; Trigo et al, ).…”

Section: Discussionmentioning

confidence: 75%

Fertilization rate, motility, lipid peroxidation and pH changes after chilled storage of rainbow trout ( Oncorhynchus mykiss ) eggs and spermatozoa by a RMPI medium

İnanan

2019

Aquac Res

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The aim of this study was to evaluate the effects of Roswell Park Memorial Institute (RPMI) 1,640 medium on chilled storage of eggs and spermatozoa of rainbow trout (Oncorhynchus mykiss). After 3 days of storage, eggs in RPMI 1,640 media (pH 8.2, 9 and 10), Cortland medium and coelomic fluid were inseminated with fresh spermatozoa (Experiment 1). Eggs in RPMI 1,640 medium at pH 8.2 shown the lowest thiobarbituric acid‐reactive substances (TBARS, 0.053 ± 0.003 nmol/ml) and pH changes (from 8.20 ± 0.01 to 8.18 ± 0.01), the highest fertilization rate (82 ± 3%). Undiluted and diluted spermatozoa at ratios of 1:2 and 1:9 with RPMI 1,640 media (pH 8.2, 9 and 10) and Cortland medium were inseminated with fresh eggs (Experiment 2). Spermatozoa in RPMI 1,640 medium at pH 9 (1:9) caused the lowest TBARS content (0.037 ± 0.002 nmol/ml) and pH changes (from 9.00 ± 0.01 to 8.98 ± 0.01), the highest fertilization rate (77 ± 2%) and motility parameters. Based on Experiments 1 and 2, eggs and spermatozoa were stored for another 3 days in RPMI 1,640 medium at pH 8.2 and 9 (1:9) respectively (Experiment 3). Fertilization rate of storage eggs and spermatozoa in Experiment 3 was 79 ± 5%, showing successfully storage of rainbow trout gametes with the same medium.

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“…Storage of refrigerated semen is a simple and inexpensive procedure (Shaliutina et al., ) that can facilitate the management and reproduction programmes in aquaculture and also represent a useful strategy for preservation of threatened or endangered fish (Aguilar‐Juárez et al., ). However, there is a significantly decrease on sperm function after short‐term storage of fish semen (Lahnsteiner et al, ; Vuthiphandchai et al, ; Aramli et al., ; Shaliutina et al., ; Aguilar‐Juárez et al., ; Trigo et al., ) mainly due to oxidative stress (Lahnsteiner et al., ; Aramli et al., ; Shaliutina et al., ). The imbalance between the production of reactive oxygen species (ROS) and a biological systems ability to readily detoxify the reactive intermediates or easily repair the resulting damage is known as oxidative stress (Agarwal et al., ).…”

Section: Introductionmentioning

confidence: 99%

Short-term storage of salmonids semen in a sodium alginate-based extender

et al. 2016

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Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our study was to analyse the effect of a sodium alginate-based extender on sperm function in the short-term storage of salmonids semen. Samples of Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss were stored in Storfish (Ext-C) and Storfish supplemented with sodium alginate (Ext-A) during 10 days at 4°C. After storage, motility, viability, mitochondrial membrane potential (ΔΨmit), superoxide anion (O ) level and DNA fragmentation (DNA Frag) were assessed. Ext-A had positive effect in preservation of sperm motility, viability, ΔΨmit, O level and DNA integrity in the three species analysed compared to control samples. In Ext-A, the spermatozoa of S. salar and O. mykiss showed significantly higher motility, viability and ΔΨmit than O. kisutch. However, O. kisutch and O. mykiss had significantly lower O level than S. salar, and DNA fragmentation in O. kisutch and S. salar was significantly lower than in samples of O. mykiss (p < 0.05). Dilution of salmonids semen in a sodium alginate-based extender is effective for protecting sperm quality during 10 days of short-term storage.

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Cold storage of the sperm of the endemic trout Oncorhynchus mykiss nelsoni: a strategy for short-term germplasm conservation of endemic species

Cited by 16 publications

References 50 publications

Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)

Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)

Fertilization rate, motility, lipid peroxidation and pH changes after chilled storage of rainbow trout ( Oncorhynchus mykiss ) eggs and spermatozoa by a RMPI medium

Short-term storage of salmonids semen in a sodium alginate-based extender

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