Cold Stress Evaluation among Maize (<i>Zea mays</i> L.) Inbred Lines in Different Temperature Conditions

Farooqi, Muhammad Qudrat Ullah; Lee, Ju Kyong

doi:10.9787/pbb.2016.4.3.352

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Much research has been done on phenotypic evaluation of cold tolerance, but few studies have used a genetic approach to identify cold tolerant maize (Hoffman et al, 2015). In a previous study by Farooqi and Lee (2016), maize inbred lines were separated into cold tolerant or cold susceptible lines. In the present study, among eight inbred lines, five were selected for high cold tolerance and three were considered as poorly tolerant (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Bulk segregant analysis (BSA) for improving cold stress resistance in maize using SSR markers

Farooqi¹,

J²,

Hong

et al. 2016

Genet. Mol. Res.

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ABSTRACT. In this study, eight inbred maize lines with high or low tolerance to cold temperature were investigated using bulk segregant analysis (BSA). Genetic diversity and genetic relationships were investigated using 100 simple sequence repeat (SSR) markers linked to cold tolerance in maize. A total of 319 alleles were identified. Among these, 128 were high coldtolerant-specific alleles and 61 were poorly coldtolerant-specific alleles, while the remaining 130 were shared between the two types. The number of alleles per locus ranged from 2 to 5, with an average of 3.19. The major allele frequency varied from 0.39 to 0.53 with an average of 0.47. The average gene diversity and polymorphic information content among all lines were 0.63 and 0.58, respectively. A dendrogram analysis identified three main clusters and most of the high tolerant inbred lines were clearly distinguished from the poorly tolerant inbred lines. In addition to the BSA, a total of 84 SSR markers were identified as high cold tolerance-specific alleles and 52 SSRs were detected as poorly cold tolerance-specific alleles. Of these, bnlg1273, umc1124, dupssr21, mmc0251, mmc0181, and phi041 have great potential for being molecular markers for cold tolerance in maize. Our results were in agreement with results previously reported for SSR markers linked with cold tolerance in maize. The identification and characterization of high and poorly cold tolerant maize lines based on SSR markers will be useful for future maize breeding studies.

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Section: Discussionmentioning

confidence: 99%

Bulk segregant analysis (BSA) for improving cold stress resistance in maize using SSR markers

Farooqi¹,

J²,

Hong

et al. 2016

Genet. Mol. Res.

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“…Several authors delineated have been conducted to determine the relative amounts of genetic variability for cold tolerance in corn in controlled growth chambers, predominantly targeting on germination and early growth stages [11,12,5,13].…”

Section: Genetic Component Of Variationmentioning

confidence: 99%

Cold Stress Tolerance Evaluation in Maize Double Haploid Genotypes

Shikha

Shahi

Singh

et al. 2022

IJPSS

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The experimental materials consisting 218 maize double haploids and two commercial checks were screened at CIMMYT-BISA-Ludhiana-Punjab under natural cold stress (winter sowing) during rabi 2019-20. Data was recorded on leaf necrosis (seedling stage), ear leaf length, ear leaf width, ear leaf area, number of plants per plant, ears per plot, bareness and grain yield. These traits were observed to be highly correlated with cold tolerance and also exhibited moderate to high heritability except leaf width, bareness. DH_1_24, DH_1_67, DH_1_216, DH_1_17 and DH_1_180 are suitable for sowing in the winter season. Leaf necrosis observed as important criteria for selection of cold tolerant genotypes at vegetative stage and bareness at reproductive stage.

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“…Imbibition is the uptake of water by the dry seed, a physical process dependent on differences in water potential (BEWLEY, 1977;HADAS, 2005), and not much is known about the effect of low temperatures on this phase. Since maize varieties respond differently to low temperatures, there is substantial variability in regards to low temperature tolerance in different maize genotypes (FAROOQI and LEE, 2016;MILIVOJEVIĆ et al, 2017). Understanding the different ways in which they respond to this abiotic factor during the earlier stages of development, including imbibition, is crucial.…”

Section: Introductionmentioning

confidence: 99%

Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions

et al. 2021

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Maize is one of the most important crop species worldwide, but also extremely susceptible to the effects of increasingly higher temperatures and drought during the summer and its flowering and grain filling stage. Different strategies are being utilized to ensure a satisfying yield potential and quality even in the extremely unfavourable environmental conditions, which are the result of climate change. Some of them are cropping pattern changes and sowing alterations, including earlier sowing. Since this implies the exposure to suboptimal temperatures during early developmental stages, it leads to a demand for developing maize lines tolerant to low temperatures during these stages. This research focuses on the first phase of germination, imbibition. Maize tolerance to low temperatures is a complex trait that includes different mechanisms and strategies that all work together to ensure adaptation and survival, such as cell membrane changes, antioxidative system activation, etc. Additionally, the low temperature response of different maize inbreds varies substantially, so recognizing the different ways in which they respond to low temperatures during imbibition and other earlier stages of development is crucial. To accomplish this, seeds of two maize inbred lines of contrasting susceptibility to low temperatures were selected and exposed to control (20? C) and low temperature (8?C) conditions during the first 24h of imbition and then further analyzed to assess their response. This included germination and tetrazolium assays; ascertaining membrane integrity by evaluating cell leakage and lipid peroxidation; determining the antioxidative capacity by assessing superoxide dismutase (SOD) and catalase (CAT) activity; and expression analysis of four genes included in the low temperature response (gibberellin insensitive dwarf 1 gibberellin receptor, gid1; fatty acid desaturases 2 and 6, fad2 and fad6; plastid-lipid-associated 2 protein, pap2). The results showed that, while there is not a significant difference in their germination rate, they differ in their survival rate, with more seeds of the tolerant genotype surviving the low temperature period. Significant differences between them were found in cell leakage (p<0,01), as well as gid1(p<0,05) and fad6 (p<0,05) gene expression assays. The present research brings light to our understanding of the effect of low temperatures on the first germination stage, - imbibition. It highlights the importance of choosing the right inbreds for earlier sowing and points to certain routes that could be taken for improving and accelerating the breeding process for low temperature tolerance.

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Cold Stress Evaluation among Maize (Zea mays L.) Inbred Lines in Different Temperature Conditions

Cited by 6 publications

References 21 publications

Bulk segregant analysis (BSA) for improving cold stress resistance in maize using SSR markers

Bulk segregant analysis (BSA) for improving cold stress resistance in maize using SSR markers

Cold Stress Tolerance Evaluation in Maize Double Haploid Genotypes

Physiological and gene expression changes during imbibition in maize seeds under low temperature conditions

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