Cold-stress-induced de novo expression of <i>infC</i> and role of IF3 in cold-shock translational bias

Giuliodori, Anna Maria; Brandi, Anna; Giangrossi, Mara; Gualerzi, Claudio O.; Pon, Cynthia L.

doi:10.1261/rna.455607

Cited by 54 publications

(54 citation statements)

References 39 publications

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“…In E. coli, cold shock leads to an increase in IF3 abundance relative to ribosome abundance, leading to the preferential translation of cold-shock mRNAs (37). At low temperature, IF3 increases the binding rate of fMet-tRNA to ribosomes associated with cold-shock mRNA, increases the pool size of 30S subunits, and lowers the fidelity of translation of non-cold-shock mRNAs (38). In Myxococcus xanthus, the IF3 family member D Fig.…”

Section: Discussionmentioning

confidence: 99%

Unique role for translation initiation factor 3 in the light color regulation of photosynthetic gene expression

Gutu

Nesbit²,

Alverson

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Significance The regulation of photosynthesis is important, yet poorly understood. Our work reveals a previously undescribed form of photosynthesis gene regulation in cyanobacteria that apparently also controls gene expression in plants, including commercially important crops. This finding may provide a unique approach to modifying the environmental responses and developmental programs of agriculturally important species. In addition, translation is a key biological process, and many of its important features and regulation remain to be described. Initiation is the rate-limiting step of translation, making it a critical point for regulation. Our finding that translation initiation factor 3 families exist with common roles in initiation and individual roles in regulating gene expression provides additional insights into the translation mechanism and its regulation.

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Section: Discussionmentioning

confidence: 99%

Unique role for translation initiation factor 3 in the light color regulation of photosynthetic gene expression

Gutu

Nesbit²,

Alverson

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…Moreover, it has been described for E. coli that preferential translation of the csp genes occurs at low temperature (18,19,20,24). The 5=-UTRs of cspA and cspB mRNAs from C. crescentus share a region of sequence similarity of 13 nt (called upstream box), whose relevance for regulation was evaluated in expression assays using translational fusions.…”

Section: Discussionmentioning

confidence: 99%

Cold Shock Genes cspA and cspB from Caulobacter crescentus Are Posttranscriptionally Regulated and Important for Cold Adaptation

et al. 2012

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Cold shock proteins (CSPs) are nucleic acid binding chaperones, first described as being induced to solve the problem of mRNA stabilization after temperature downshift. Caulobacter crescentus has four CSPs: CspA and CspB, which are cold induced, and CspC and CspD, which are induced only in stationary phase. In this work we have determined that the synthesis of both CspA and CspB reaches the maximum levels early in the acclimation phase. The deletion of cspA causes a decrease in growth at low temperature, whereas the strain with a deletion of cspB has a very subtle and transient cold-related growth phenotype. The cspA cspB double mutant has a slightly more severe phenotype than that of the cspA mutant, suggesting that although CspA may be more important to cold adaptation than CspB, both proteins have a role in this process. Gene expression analyses were carried out using cspA and cspB regulatory fusions to the lacZ reporter gene and showed that both genes are regulated at the transcriptional and posttranscriptional levels. Deletion mapping of the long 5=-untranslated region (5=-UTR) of each gene identified a common region important for cold induction, probably via translation enhancement. In contrast to what was reported for other bacteria, these cold shock genes have no regulatory regions downstream from ATG that are important for cold induction. This work shows that the importance of CspA and CspB to C. crescentus cold adaptation, mechanisms of regulation, and pattern of expression during the acclimation phase apparently differs in many aspects from what has been described so far for other bacteria.

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“…Cold shock causes the single E. coli IF3 to increase in abundance relative to ribosome abundance, causing the preferential translation of cold-shock mRNAs (Giuliodori et al 2004). At low temperature, IF3 increases the binding rate of fMet-tRNA to ribosomes that are associated with cold-shock mRNAs, increases the pool size of 30S subunits, and also lowers the translation fidelity of non-cold-shock mRNAs (Giuliodori et al 2007). In Myxococcus xanthus, the IF3 family member D signal (Dsg) is involved in both translation initiation and developmental processes, including the control of sporulation and the development of fruiting bodies Kalman et al 1994).…”

Section: Use Of Multiple If3s In F Diplosiphon and Perhaps Other Bacmentioning

confidence: 99%

Translation initiation factor 3 families: what are their roles in regulating cyanobacterial and chloroplast gene expression?

et al. 2015

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Initiation is a key control point for the regulation of translation in prokaryotes and prokaryotic-like translation systems such as those in plant chloroplasts. Genome sequencing and biochemical studies are increasingly demonstrating differences in many aspects of translation between well-studied microbes such as Escherichia coli and lesser studied groups such as cyanobacteria. Analyses of chloroplast translation have revealed its prokaryotic origin but also uncovered many unique aspects that do not exist in E. coli. Recently, a novel form of posttranscriptional regulation by light color was discovered in the filamentous cyanobacterium Fremyella diplosiphon that requires a putative stem-loop and involves the use of two different prokaryotic translation initiation factor 3s (IF3s). Multiple (up to five) putative IF3s have now been found to be encoded in 22 % of sequenced cyanobacterial genomes and 26 % of plant nuclear genomes. The lack of similar light-color regulation of gene expression in most of these species suggests that IF3s play roles in regulating gene expression in response to other environmental and developmental cues. In the plant Arabidopsis, two nuclear-encoded IF3s have been shown to localize to the chloroplasts, and the mRNA levels encoding these vary significantly in certain organ and tissue types and during several phases of development. Collectively, the accumulated data suggest that in about one quarter of photosynthetic prokaryotes and eukaryotes, IF3 gene families are used to regulate gene expression in addition to their traditional roles in translation initiation. Models for how this might be accomplished in prokaryotes versus eukaryotic plastids are presented.

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Cold-stress-induced de novo expression of infC and role of IF3 in cold-shock translational bias

Cited by 54 publications

References 39 publications

Unique role for translation initiation factor 3 in the light color regulation of photosynthetic gene expression

Unique role for translation initiation factor 3 in the light color regulation of photosynthetic gene expression

Cold Shock Genes cspA and cspB from Caulobacter crescentus Are Posttranscriptionally Regulated and Important for Cold Adaptation

Translation initiation factor 3 families: what are their roles in regulating cyanobacterial and chloroplast gene expression?

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