Cold stress modulates osmolytes and antioxidant system in Calendula officinalis

Jan, Nelofer; Majeed, Umer; Andrabi, Khurshid Iqbal; John, Riffat

doi:10.1007/s11738-018-2649-0

Cited by 52 publications

(27 citation statements)

References 84 publications

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“…To prevent oxidative damage caused by low-temperature stress, plants eliminate or reduce excess reactive oxygen species (ROS) or ROS-induced toxic substances through the antioxidant enzyme system [54][55]. Many proteomic researchers showed that a low-temperature causes a differential expression of antioxidant defense-related proteins [56][57].…”

Section: Response Mechanism Of Lysine Crotonylation and Chrysanthemummentioning

confidence: 99%

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Lin

Bai

et al. 2020

Preprint

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Background: Low-temperature severely affects the growth and development of chrysanthemum which is one kind of ornamental plant well-known and widely used in the world. Lysine crotonylation is a recently identified post-translational modification (PTM) with multiple cellular functions. However, lysine crotonylation under low-temperature stress has not been studied. Results: Proteome-wide and lysine crotonylation of chrysanthemum at low temperature was analyzed using TMT labeling, sensitive immuno-precipitation, and high-resolution LC-MS/MS. The results showed that 2017 crotonylation sites were identified in 1199 proteins. Treatment at 4 °C for 24 h and −4 °C for 4 h resulted in 393 upregulated proteins and 500 downregulated proteins (1.2-fold threshold and P < 0.05). Analysis of biological information showed that lysine crotonylation was involved in photosynthesis, ribosomes, and antioxidant systems. The crotonylated proteins and motifs in chrysanthemum were compared with other plants to obtain orthologous proteins and conserved motifs. To further understand how lysine crotonylation at K136 affected APX (ascorbate eroxidase), we performed a site-directed mutation at K136 in APX. Site-directed crotonylation showed that lysine decrotonylation at K136 reduced APX activity, and lysine complete crotonylation at K136 increased APX activity. Conclusion: In summary, our study comparatively analyzed proteome-wide and crotonylation in chrysanthemum under low-temperature stress and provided insights into the mechanisms of crotonylation in positively regulated APX activity to reduce the oxidative damage caused by low-temperature stress. These data provided an important basis for studying crotonylation to regulate antioxidant enzyme activity in response to low-temperature stress and a new research ideas for chilling-tolerance and freezing-tolerance chrysanthemum molecular breeding.

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Section: Response Mechanism Of Lysine Crotonylation and Chrysanthemummentioning

confidence: 99%

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Lin

Bai

et al. 2020

Preprint

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“…However, activity measurements usually cannot easily discriminate among the isoforms of Mn-, CuZn- and Fe-SOD. The transcript amounts and activity of SOD increase during low-temperature treatment in Capsella bursa pastoris L. [ 9 ] and Calendula officinalis [ 10 ]. The SOD activity in Calendula officinalis rises less than the transcript amount pointing to posttranscriptional regulation of synthesis or higher protein degradation rates [ 10 , 29 ].…”

Section: Variability Of Cold Response Between Speciesmentioning

confidence: 99%

“…The transcript amounts and activity of SOD increase during low-temperature treatment in Capsella bursa pastoris L. [ 9 ] and Calendula officinalis [ 10 ]. The SOD activity in Calendula officinalis rises less than the transcript amount pointing to posttranscriptional regulation of synthesis or higher protein degradation rates [ 10 , 29 ]. Likewise, the SOD activities of Phaseolus vulgaris [ 30 ], Cynodon dactylon [ 25 ], and Withania somnifera [ 31 ] only increase slightly upon exposure to low temperature.…”

Section: Variability Of Cold Response Between Speciesmentioning

confidence: 99%

“…A similar increase of SOD and CAT activity was observed C. bursa-pastoris [ 9 ], Citrullus lanatus [ 34 ], and C. dactylon [ 25 ]. Therefore, SOD and CAT activity could play an important part during chilling stress, although some species like C. officinalis [ 10 ] and P. vulgaris [ 30 ] fail to increase the activity of these enzymes. The increased CAT is likely important to avoid accumulation of H 2 O 2 if its production increases under cold due to imbalances and changes in metabolism.…”

Section: Variability Of Cold Response Between Speciesmentioning

confidence: 99%

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Reactive Oxygen Species and the Redox-Regulatory Network in Cold Stress Acclimation

2018

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Cold temperatures restrict plant growth, geographical extension of plant species, and agricultural practices. This review deals with cold stress above freezing temperatures often defined as chilling stress. It focuses on the redox regulatory network of the cell under cold temperature conditions. Reactive oxygen species (ROS) function as the final electron sink in this network which consists of redox input elements, transmitters, targets, and sensors. Following an introduction to the critical network components which include nicotinamide adenine dinucleotide phosphate (NADPH)-dependent thioredoxin reductases, thioredoxins, and peroxiredoxins, typical laboratory experiments for cold stress investigations will be described. Short term transcriptome and metabolome analyses allow for dissecting the early responses of network components and complement the vast data sets dealing with changes in the antioxidant system and ROS. This review gives examples of how such information may be integrated to advance our knowledge on the response and function of the redox regulatory network in cold stress acclimation. It will be exemplarily shown that targeting the redox network might be beneficial and supportive to improve cold stress acclimation and plant yield in cold climate.

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“…SOD being the first line of defense in the multifaceted enzymatic antioxidant defense systems, plays a significant role in converting O 2 * to O 2 and H 2 O 2 . Afterwards H 2 O 2 is broken down by POD, and CAT to H 2 O [70,75,76]. Several environmental perturbations in plants lead to higher antioxidant enzyme levels [72] correspondingly a substantial surge in CAT, POD, and SOD, activities were perceived in P. dulce plants after exposure to fly ash stress (Figure 3b).…”

Section: Resultsmentioning

confidence: 99%

Fly-Ash Pollution Modulates Growth, Biochemical Attributes, Antioxidant Activity and Gene Expression in Pithecellobium Dulce (Roxb) Benth

Qadir

Raja

Siddiqui

et al. 2019

Plants

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This study investigates the effect of fly ash (FA) on the Pithecellobium dulce (Roxb) Benth. trees growing at three different locations. FA stress caused significant changes in different leaf attributes like sugar, protein contents, photosynthetic pigments, nitrate content and nitrate reductase activity in foliar tissues of plants growing at a highly contaminated site, as compared to a low-pollution site. Lower rates of stomatal conductance (SC) were observed in P. dulce leaves under fly ash stress conditions that drastically reduced net photosynthetic rate (PN); however, intercellular carbon dioxide concentration and stomatal index (SI) showed an increase under the same stress conditions. On the other hand, significant increase was also observed in the proline, sulphur and nitrogen contents. A significant increase in oxidative stress and, consequently, in antioxidant enzymes such as ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxidase dismutase (SOD) and Air pollution tolerance index were discovered at three different sites. The transcriptional expression of antioxidant and stress responsive genes was higher at HPS as compared to two other two sites of the study. Taken together the results demonstrated that the P. dulce is best suited as a fly ash stress tolerant plant species with the potential to provide an alternative for the reclamation of fly ash affected soils.

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Cold stress modulates osmolytes and antioxidant system in Calendula officinalis

Cited by 52 publications

References 84 publications

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature

Reactive Oxygen Species and the Redox-Regulatory Network in Cold Stress Acclimation

Fly-Ash Pollution Modulates Growth, Biochemical Attributes, Antioxidant Activity and Gene Expression in Pithecellobium Dulce (Roxb) Benth

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