Colicin Concentrations Inhibit Growth of Escherichia coli O157:H7 In Vitro

Callaway, Todd R.; Stahl, Chad H.; Edrington, Thomas S.; Genovese, Kenneth J.; Lincoln, Leslie M.; Anderson, Robin C.; Lonergan, Steven M.; Poole, Toni L.; Harvey, Roger B.; Nisbet, David J.

doi:10.4315/0362-028x-67.11.2603

Cited by 22 publications

(17 citation statements)

References 24 publications

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“…Thus, directly inoculated pathogens in wastewater declined rapidly, with a decimal reduction time of 3.5 days, while they persisted inside 300-kDa dialysis tubes (decimal reduction time of 18.5 days). Yet the significant decline in pathogen numbers in 1-kDa tubes compared to that in the high-molecular-weight-cutoff tube is unexpected, although the pathogen may also be protected from colicins (8) and antibiotics produced from native organisms in wastewater. Exclusion of nutrients and growth factors of Ͼ1 kDa is hypothesized for the pathogen decline inside the low-molecular-weight-cutoff dialysis tube.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, feeding cattle with a straw-rich diet (12), silage made with bacteriocin-producing bacteria (3), and competitive exclusion products using lactic acid bacteria (7), colicinogenic E. coli (8,33) and O157-specific bacteriophages (35) has the potential to inhibit pathogen replication inside the animals. Nevertheless, releasing even a few cells into environments that favor pathogen regrowth (10) may pose an epidemiological risk.…”

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confidence: 99%

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Extractable Organic Components and Nutrients in Wastewater from Dairy Lagoons Influence the Growth and Survival of Escherichia coli O157:H7

Ravva

Korn

2007

Appl Environ Microbiol

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The influence of nutrients in wastewater from dairy lagoons on the survival of Escherichia coli O157:H7 was monitored. Initially, the survival of E. coli O157:H7 in wastewater from which the competing native organisms had been removed by filter sterilization or autoclaving was compared with that in wastewater from which competing organisms had not been removed. Numbers of E. coli O157:H7 or E. coli ONT (O-nontypeable):H32 cells declined rapidly in filter-sterilized water and exhibited a slower decline in nonsterile water, while the organisms proliferated in autoclaved water. Subsequently, the growth of E. coli O157:H7 strains was monitored in 300 l of Luria-Bertani (LB) broth supplemented with incremental proportions of filter-sterilized wastewater. E. coli O157:H7 and E. coli ONT:H32 strains failed to grow in filter-sterilized wastewater, and their growth was reduced incrementally with wastewater supplementation of LB broth. Consequently, the influence of organic extracts of wastewater on the growth of E. coli O157:H7 and E. coli ONT:H32 in reduced-strength LB was monitored, followed by scale-up tests in wastewater. Acidic and basic extracts inhibited growth of both strains, while the neutral aqueous extract improved growth. However, a scale-up with a threefold increase in the acidic components supplementing the wastewater did not result in any additional decline in numbers of E. coli O157:H7 cells. When protected inside a 300-kDa dialysis tube and exposed to diffusible components, E. coli O157:H7 survived longer, with a decimal reduction time of 18.1 days, compared to 3.5 days when inoculated directly into wastewater. Although wastewater can potentially provide nutrients to naturally occurring human pathogens, the chemical components, protozoa, and coliphages in wastewater can inhibit the growth of freshly introduced pathogens from manure.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Extractable Organic Components and Nutrients in Wastewater from Dairy Lagoons Influence the Growth and Survival of Escherichia coli O157:H7

Ravva

Korn

2007

Appl Environ Microbiol

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“…Supershedders are cattle that excrete the pathogen at concentrations of Ն10 4 CFU · g Ϫ1 of feces (5); they are thought to account for a small proportion of the cattle population and yet may contribute 80 to 96% of the total load of E. coli O157:H7 in the herd (5)(6)(7). Multiple factors contribute to shedding of E. coli O157:H7 in cattle, including diet (8), housing (9), season (10), stress (11), and age (12). However, knowledge of the microbial ecology of the gastrointestinal tract of cattle is limited, and relationships among microbiota may impact the ability of E. coli O157:H7 to persist in some cattle (13).…”

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confidence: 99%

Differing Populations of Endemic Bacteriophages in Cattle Shedding High and Low Numbers of Escherichia coli O157:H7 Bacteria in Feces

Hallewell

Niu

Munns

et al. 2014

Appl Environ Microbiol

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The objectives of this study were to identify endemic bacteriophages (phages) in the feedlot environment and determine relationships of these phages to Escherichia coli O157:H7 from cattle shedding high and low numbers of naturally occurring E. coli O157:H7. Angus crossbred steers were purchased from a southern Alberta (Canada) feedlot where cattle excreting >10 4 CFU · g ؊1 of E. coli O157:H7 in feces at a single time point were identified as supershedders (SS; n ‫؍‬ 6), and cattle excreting <10 4 CFU · g ؊1 of feces were identified as low shedders (LS; n ‫؍‬ 5). Fecal pats or fecal grabs were collected daily from individual cattle for 5 weeks. E. coli O157:H7 in feces was detected by immunomagnetic separation and enumerated by direct plating, and phages were isolated using short-and overnight-enrichment methods. The total prevalence of E. coli O157:H7 isolated from feces was 14.4% and did not differ between LS and SS (P ‫؍‬ 0.972). The total prevalence of phages was higher in the LS group (20.9%) than in the SS group (8.3%; P ‫؍‬ 0.01). Based on genome size estimated by pulsed-field gel electrophoresis and morphology determined by transmission electron microscopy, T4-and O1-like phages of Myoviridae and T1-like phage of Siphoviridae were isolated. Compared to T1-and O1-like phages, T4-like phages exhibited a broad host range and strong lytic capability when targeting E. coli O157:H7. Moreover, the T4-like phages were more frequently isolated from feces of LS than SS, suggesting that endemic phages may impact the shedding dynamics of E. coli O157:H7 in cattle.

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“…has allowed colicins to be produced in greater amounts from different recombinant host systems so that proof-of-concept studies could be performed (17)(18)(19). These studies demonstrated that colicins could be used to reduce populations of several species of foodborne pathogenic bacteria on food products, and in live animals (20).…”

Section: Colicins a Smart Arrowmentioning

confidence: 99%