Coliform Bacteria

Parr, Leland W.

doi:10.1128/br.3.1.1-48.1939

Cited by 52 publications

(3 citation statements)

References 122 publications

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“…The principle of using LPBR in this MPN assay is based on the typical ability of coliform bacteria to ferment lactose (Brackett, 1993; Caplenas & Kanarek, 1984; Parr, 1939). LPBR only provides lactose and peptone as potential energy sources, and the inclusion of rifampicin limits the growth of rifampicin‐susceptible bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…Once confirmed, the LPBR MPN assay can be used to accurately measure other rifampicin‐resistant E. coli strains or lactose fermenters, provided the rifampicin resistance is conserved. If rifampicin is removed from the assay, LPB MPN assay can be modified for assessment of lactose fermenting bacteria, in which coliforms are a representative group (Brackett, 1993; Caplenas & Kanarek, 1984; Parr, 1939). The typical coliform identification method also recommends including verification through gas production by adding inverted Durham tubes to each well/test tube (Brackett, 1993; Parr, 1939).…”

Section: Discussionmentioning

confidence: 99%

“…If rifampicin is removed from the assay, LPB MPN assay can be modified for assessment of lactose fermenting bacteria, in which coliforms are a representative group (Brackett, 1993; Caplenas & Kanarek, 1984; Parr, 1939). The typical coliform identification method also recommends including verification through gas production by adding inverted Durham tubes to each well/test tube (Brackett, 1993; Parr, 1939). Thus, the 3‐tube MPN assay in glass tubes with LPB and inverted Durham tubes is a potential application for total coliform estimates.…”

Section: Discussionmentioning

confidence: 99%

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Development and evaluation of a modified most probable number (MPN) method for enumerating rifampicin‐resistant Escherichia coli in agricultural, food, and environmental samples

Gao,

Jha,

Hudson

et al. 2024

Journal of Food Safety

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Low level of rifampicin‐resistant E. coli is frequently used in food safety research as a model microorganism and is typically enumerated using traditional Most Probable Number (MPN) method. To simplify the process, we developed and validated a modified MPN method based on lactose peptone broth (LPB) containing rifampicin and bromocresol purple, the broth changes to a yellow color due to pH shifts induced by lactose fermentation from E. coli. E. coli TVS353 were prepared in following suspensions including healthy cells, injured cells, competitive bacteria with or without rifampicin resistance, and unknown natural microflora. E. coli TVS353 was quantified by traditional MPN method and our improved method. At a predicted level of 1 log CFU/mL, there was no significant difference in the MPN index of LPBR compared with TSB and TSBR in these suspensions (p > 0.05). Confirmation results generated from this new color change MPN method were analogous to traditional MPN methods.

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Section: Resultsmentioning

confidence: 99%