Coliform organisms associated with milk of cows with mastitis and their sensitivity to commonly available antibiotics in Kaduna State, Nigeria

Abstract

An examination of raw milk for the isolation of coliforms as members of mastitis-causing organisms was conducted on 300 cows from four Local Government Areas in Kaduna State, northern Nigeria. A 10.3% prevalence was recorded for coliform organisms using the Microgen GN-ID A+B Kit (MedicaTec TM ), these were; Enterobacter spp., Citrobacter spp., Klebsiella spp., Serratia marcescens, Proteus spp. and Pantoea agglomerans (similar to Enterobacter aerogenes). Antibiotic sensitivity test using commonly available antibiotics showed that all isolates were sensitive to amoxicillin and ciprofloxacin, but resistant to tetracycline and erythromycin. Age, parity number, stage of lactation, management system, hygiene of milking process, and presence of lesion on udder/teat were found to be significantly associated (p<0.05) with the prevalence of mastitis in cows. The lowest prevalence (24%; 48 of 200) was recorded in cows within 3 to 4 years of age while, the highest (60.6%; 20 of 33) was in cows aged above 5 years. Stage of lactation was significant with the prevalence of mastitis being the highest (45.5%; 30 of 66) during the initial stage of lactation (0 to 5 month). It was concluded that, the relatively high prevalence of coliforms in bovine mastitis in dairy herds could significantly reduce milk production and cause economic losses. Good hygiene in milking process, milking clinically infected cows last, culling chronic mastitis cases, treating clinically infected cows and dry period therapy could reduce the prevalence of coliform mastitis in Kaduna State, Nigeria.

Untitled

“…In this study, a prevalence of 19.7% has been established for subclinical mastitis at cow level. This is lower than 29.7% reported in Kaduna State, Nigeria [12], 30.9% in Plateau State, Nigeria [13], 85.33% in Sokoto State, Nigeria [2], 64.1% in Egypt [11], and 52.3% in China [23]. However, it is higher than 10.5% reported in Iran [24].…”

“…All suspected coliform bacterial isolates that stained red with Gram reaction were subjected to Conventional biochemical tests. The tests conducted were: Indole, Methyl Red, Voges-Proskauer and Citrate Utilization (IMViC) according to standard microbiological techniques described by Cheesborough [22] and Mbuk, et al [12]. The suspected coliform bacterial isolates from the tests were identified up to species level using Microgen A+B Kit (UK) in accordance with the manufacturer's instructions.…”

“…It affects every country, including developed ones and causes huge financial losses [9]. There is agreement among authors that mastitis is the most widespread infectious diseases in dairy cattle, and, from an economic aspect, the most damaging [2,[10][11][12][13][14].…”

Molecular Studies on E.Coli Isolate from Milk of Mastatic Cattle with Special Reference to Associated Biochemical Changes in Kaliobea Governorate

“…Virulence factors of mammary pathogenic E. coli (MPEC) involved pathogenesis of mastitis as well as those differentiating strains causing acute or persistent mastitis [4]. The infection occur after bacteria entrance mammary gland via teat canal ,overcoming anatomical battier so they must evade the cellular and humeral defence mechanism of mammary gland to establish disease Radostits, et al [5] and Mbuk, et al [6]. Limited number of E.coli strains has ability to adhere and invade bovine mammary epithelial cell[s and cause persistant infection ,have severa l fimberia and fimberial adhesion that mediat adhesion to host epithelial cell through cell surface Milanov, et al [7] and Döpfer, et al [8].…”

Molecular Studies on E.coli Isolate from Milk of Mastatic Cattle with Special Reference to Associated Biochemical Changes in Kaliobea Governorate

“…Virulence factors of mammary pathogenic E. coli (MPEC) involved pathogenesis of mastitis as well as those differentiating strains causing acute or persistent mastitis [4]. The infection occur after bacteria entrance mammary gland via teat canal, overcoming anatomical battier so they must evade the cellular and humeral defence mechanism of mammary gland to establish disease [5,6]. Limited number of E.coli strains has ability to adhere and invade bovine mammary epithelial cell[s and cause persistant infection, have severa l fimberia and fimberial adhesion that mediat adhesion to host epithelial cell through cell surface [7,8].…”